Vitamin E supplementation ameliorates aflatoxin B1-induced nephrotoxicity in rats

Ahmed A.M. Abdel-Hamid, Alaa El-Din L. Firgany
  • Acta Histochemica, October 2015, Elsevier
  • DOI: 10.1016/j.acthis.2015.08.002

What is it about?

• Aflatoxin B1 (AFB1) induces renal tubular and glomerular injury. • AFB1 provokes oxidative stress in the renal tissue. • Vitamin E alleviates AFB1-induced renal damage.

Why is it important?

Fungal toxins in nutrition can cause organ dysfunction or even failure. Aflatoxin B1 (AFB1)-induced renal impairment is not sufficiently studied regarding its extent and prevention. The aim of this experiment was to study the effect of AFB1 on renal cortical tissue and whether its possible harmful effect could be prevented by the conventional economical antioxidant, vitamin E. Forty rats were divided into four groups; I–IV. Group I represented the control while the others received vitamin E (Vit E), AFB1 and AFB1 + Vit E, respectively. Renal cortex specimens were taken from each group after 25 days. Then, specimens were prepared for histological study by hematoxlyin and eosin (H&E), Masson's trichrome, caspase-3 as well as for ultrastructural examination and oxidative stress parameters evaluation. Data were morphometrically and statistically analyzed. In AFB1-treated group, focal tubulo-interstitial affection in the form of tubular cytoplasmic vacuolation, mitochondrial disruption, numerous lysosomes, marked increase in collagen deposition and in caspase-3 expression were observed. Glomerular impairment in the form of fusion of podocytes enlarged foot processes and thickening of the glomerular basement membrane (GBM) with loss of its trilaminar appearance were detected. In the group treated by AFB1 + Vit E, there were minimal affection of the histological structure of the renal cortex as well as significant increase in the anti-oxidative parameters which were significantly decreased in the AFB1-treated group. Therefore, Vit E could be considered in wide experimental studies to be a first choice antioxidant of high cost-effectiveness in prevention of fungal toxins pro-oxidant-induced renal impairment.

Perspectives

Dr. Ahmed A.M. Abdel-Hamid (Author)
Mansoura University

Keywords Aflatoxin B1; Nephrotoxicity; Vitamin E; Caspase-3; Ultrastructure

The following have contributed to this page: Dr. Ahmed A.M. Abdel-Hamid