Alteration in relative activities of phenylalanine dehydrogenase towards different substrates by site-directed mutagenesis

Stephen Y.K. Seah, K. Linda Britton, Patrick J. Baker, David W. Rice, Yasuhisa Asano, Paul C. Engel
  • FEBS Letters, August 1995, Wiley
  • DOI: 10.1016/0014-5793(95)00804-i

What is it about?

Sequence comparisons established the relatedness of several amino acid dehydrogenases. The existence of a solved structure for GluDH made it possible to interpret key differences by homology modelling. In this paper we used predictions that two residues were primarily responsible for specificity difference between LeuDH and PheDH. Single and double mutations validated the prediction

The following have contributed to this page: Professor Paul C Engel