What is it about?
Proanthocyanidin (PAC) is an anti-cancer drug that effectively treats various cancers, including colon cancer. Whereas, the chemotherapeutic potential has restricted to low bioavailability and more defects. To overcome these complications, PAC produces a selective intend of AgNPs as a medicine for cancer cells and growing modern antineoplastic promoter, an innovative nano-capped drug has enhanced the bioavailability of tumor cells. The synthesized PAC-AgNPs was described by spectroscopy analysis. The phenolic compound has hydroxyl groups which are able to bind to metals and reduce the metal salt and provide stability against agglomeration was confirmed by FTIR and spherical or polygonal shape with an average size of 72.35 nm found through FE-SEM analysis, applicable for targeted delivery to specific tissues. The potential of this nano incorporates drug had been caused apoptosis in colon cancer cells was measured. Significantly, it was found in decreasing cell growth by MTT assay, morphological changes such as membrane blebbing and chromatin condensation exhibited in AO/EB staining. In the end, the mitochondrial pathway of apoptotic cell death confirmed by cell cycle progression as well as annexin V/PI. These results strongly suggest that PAC-AgNPs could be used as a major nano-composite therapeutic drug for effectively treated with colon cancer.
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Why is it important?
PAC Capped Silver Nanoparticles Promote Apoptosis in HT-29
Perspectives
In view of the above and as a part of our ongoing research work on the synthesis of AgNPs testing the substantial process and the nanoparticles were identified through UV– Vis spectrophotometry, Fourier transform infrared spectroscopy (FTIR), X-ray powder diffraction (XRPD), Field Emission Scanning Electron Microscopy (FE-SEM), and Energy-Dispersive Spectrometric analysis (EDAX). The incorporated nanoparticles obtained and test for their antibacterial actions against Pseudomonas aeruginosa, Klebsiella pneumoniae, and Salmonella typhi adopting the well, diffusion method. Moreover, the cytotoxic effects of synthesized PAC-AgNPs and their in vitro anti-cancerous applications were studied with a mechanistic pathway on the the highest invasive cell line of human colon cancer (HT-29) cells.
Dr Paranthaman Vijayan
University of KwaZulu-Natal
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This page is a summary of: Antineoplastic Effect of PAC Capped Silver Nanoparticles Promote Apoptosis in HT-29 Human Colon Cancer Cells, Journal of Cluster Science, February 2019, Springer Science + Business Media,
DOI: 10.1007/s10876-019-01510-1.
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