What is it about?
This paper describes methyl green fluorescent properties, and identifies this far-red fluorescent stain as a promising alternative to other DNA stains.
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Why is it important?
We identified methyl green as a highly photostable DNA stain for fluorescent labeling in cells and tissues. Moreover, we describe its use as a safer alternative to ethidium bromide for staining DNA in electrophoresis gels, and its relevance for in vitro studies using fluorometry. Finally we found that methyl green behaves as a vital stain that can be used reliably for flow cytometry.
Perspectives
Methyl green fluorescence is a promising alternative to other well-established stains for confocal microscopy and cytometry, it is highly photostable and has very interesting spectral properties. Its application for DNA staining in gels has already begun spreading.
Dr Daniel Prieto
Instituto de Investigaciones Biologicas Clemente Estable
Read the Original
This page is a summary of: A fast, low cost, and highly efficient fluorescent DNA labeling method using methyl green, Histochemistry and Cell Biology, March 2014, Springer Science + Business Media,
DOI: 10.1007/s00418-014-1215-0.
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