Localization of ?-d-glucosyl and ?-d-mannosyl groups of mucosubstances with concanavalin A and horseradish peroxidase

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R. W. Stoddart (Bob) and I were research fellows at Sidney Sussex College, Cambridge, UK in the late 1960s and early 1970s. I had an interest in histochemistry, and Bob was a real carbohydrate biochemist. In discussions at a pub near the college, Bob suggested using labelled lectins as stain for localizing mucosubstances with greater selectivity than the dye-based methods of the 1950s. Bob did the protein-fluorochrome conjugations and I applied the labelled conjugates to sections of several animal tissues. My HRP-based method for detecting bound Con A by light microscopy did not collect many ciations.

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