Cereal Genomics Methods and Protocols

What is it about?

This volume of Methods in Molecular Biology is the result of the collaboration of leading scientists from around the world (including Argentina, Australia, Canada, China, France, Germany, India, Japan, Mexico, the United Kingdom, and the United States). Here, I have compiled some of the latest molecular techniques for the analysis and manipulation of grass genomes. Cutting-edge technologies and advancements in cereal genomics are covered as follows: high-throughput DNA extraction protocols, crop genetic resources, meta-quantitative trait loci (QTL) analysis, association mapping, next-generation genome sequencing, transposable element-associated variation, transcriptomics analysis, epigenetic variation (i.e., both DNA methylation and histone modifications), identification of imprinted genes, noncoding RNAs (circular RNAs), genome-editing technologies, and posttranslational protein phosphorylation. Each chapter has carefully been selected so that the reader can explore the hottest topics and apply current protocols in cereal genomics.

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Why is it important?

Major cereal crops, which include wheat, maize, rice, barley, oat, sorghum, and millet, represent primary food sources for populations around the world. Nowadays, the emergence of high-throughput sequencing technologies, along with bioinformatic tools to analyze big volumes of data and genome-editing platforms to dissect gene pathways, has modified the mode of working with genetic information and complex traits.

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