What is it about?
Senile plaque has a unique blue autofluroscence. This property was discovered in 1981. However, the exact nature of this blue autofluorescence is not clear. This publication showed that senile plaque blue autofluoresence is likely dervied from the amyloid beta self-oligomers and hetro-oligomers.
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Why is it important?
This research explains that amyloid beta peptide homo- or hetro- aggregation intrinsically carries the blue autofluoresence. The Aβ amyloid blue autofluorescence not only labels senile plaques but also illustrates red cell aggregation, hemolysis, cerebral amyloid angiopathy, vascular plaques, vascular adhesions, and microaneurysms.
Perspectives
Our results showed that Alzheimer's disease is a multi-amyloidosis disease with amyloid deposits not only in senile plaques but also in the blood lysates and damaged vascular wall. The unique amyloid beta aggregate blue autofluoresence can be used as a endogeneous tracer of amyloidosis cascades in the Alzheimer's disease
Dr. Hualin Fu
Shanghai Jiao Tong University
Read the Original
This page is a summary of: Aβ-Aggregation-Generated Blue Autofluorescence Illuminates Senile Plaques as well as Complex Blood and Vascular Pathologies in Alzheimer’s Disease, Neuroscience Bulletin, February 2024, Springer Science + Business Media,
DOI: 10.1007/s12264-023-01175-x.
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Resources
Aβ-aggregation-generated blue autofluorescence illuminates senile plaques, complex blood and vascular pathologies in the Alzheimer′s disease
Description and figures Senile plaque blue autofluorescence in the Alzheimer′s disease (AD) was discovered around 40 years ago, however, its impact on AD pathology is not fully examined. We analyzed senile plaques with immunohistochemistry and fluorescence imaging on AD brain pathological sections and also the Aβ aggregation process in vitro in test tubes. In DAPI or Hoechst staining experiments, the data showed that the nuclear blue fluorescence could only be correctly assigned after subtracting the blue autofluorescence background.
Re-evaluating the nuclear staining on pathological sections in Alzheimer's disease
A paper recently published on Nature Neuroscience, asserting that lysosome leakage induces neuronal cell death and the formation of senile plaques in Alzheimer's disease. The study believed that flower-like perikaryal rosettes formed by packed autophagic vacuoles around central nuclei, termed "PANTHOS" neurons, are responsible for the senile plaque formation. However, all of the nuclei staining in this paper based on images in the blue fluorescence channel. We did detailed studies on the senile plaque blue autofluorescence and found that senile plaque blue autofluorescence could be easily mistaken as nuclear staining. The blue "nuclear" staining in this paper is probably inaccurate since it was not verified in red or green fluorescence channels and their "nuclei"-related data might be mis-interpreted.
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