What is it about?
This assay allows to rapidly screen many samples for lipoxygenase activity. Can be used for to analyse mutant proteins obtained by site-directed mutagenesis or directed evolution.
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Why is it important?
Allows to rapidly screen the substrate specificity of lipoxygenase variants.
Perspectives
Will stimulate the use of microbial lipoxygenases for the production of industrially relevant chemicals.
Professor Willem J.H. van Berkel
Wageningen University
Read the Original
This page is a summary of: Versatile ferrous oxidation–xylenol orange assay for high-throughput screening of lipoxygenase activity, Applied Microbiology and Biotechnology, March 2024, Springer Science + Business Media,
DOI: 10.1007/s00253-024-13095-5.
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