What is it about?
Helper-dependent adenoviral vectors (HD-Ad), referred also as gutless vector, are devoid of all viral coding sequences and represent valuable tool for safe and prolonged gene expression tool for gene therapy. The current approach for generating these vectors is based on ligation to produce the viral plasmid, a procedure which is generally very inefficient. Authors describe a method for fast and efficient generation of HD-Ad vector plasmids that can accommodate expression cassettes of any size up to 35 kb. The development of this technology that makes incremental reductions in the effort and cost of preparing HD-Ad vectors is critical for clinical translation of gene therapy procedures.
Featured Image
Photo by National Cancer Institute on Unsplash
Read the Original
This page is a summary of: Generation of Helper-Dependent Adenoviral Vectors by Homologous Recombination, Molecular Therapy, February 2002, Elsevier,
DOI: 10.1006/mthe.2002.0532.
You can read the full text:
Resources
Generation of Helper-Dependent Adenoviral Vectors by Homologous Recombination
Open Access Version
Gene Therapy
Modulation of TNFalpha, a determinant of acute toxicity associated with systemic delivery of first-generation and helper-dependent adenoviral vectors
Proc Natl Acad Sci USA
Lifelong elimination of hyperbilirubinemia in the Gunn rat with a single injection of helper-dependent adenoviral vector
Contributors
The following have contributed to this page
