What is it about?
This work is about MS analysis of protein O-glycosylation. Here, deglycosylated/alkylaminylated tryptic peptides were submitted to Q-TOF MS/MS using CID, which allowed the identification of modified AA residues.
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Why is it important?
O-glycosylation represents an important posttranslational modification (PTM) of proteins, and is particularly relevant for glycoscience.
Perspectives
This method represents a simple and elegant tool for identification of O-glycosylation sites in glycoproteins. However, the deglycosylation/alkylaminylation reaction is problematic when densely glycosylated regions of glycoproteins are analyzed.
Dr Marko Haramija
University of Rijeka
Read the Original
This page is a summary of: Glycoprotein Identification and Localization of O-Glycosylation Sites by Mass Spectrometric Analysis of Deglycosylated/Alkylaminylated Peptide Fragments, Analytical Biochemistry, March 2001, Elsevier,
DOI: 10.1006/abio.2000.4955.
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