Matrix-assisted laser desorption/ionization imaging protocol forin situcharacterization of tryptic peptide identity and distribution in formalin-fixed tissue

What is it about?

This is a comprehensive guide to imaging mass spectrometry on formalin-fixed sections of tissue. The protocol paper includes all the reagents, equipment and processes required to replicate an imaging experiment on peptides produced by spray deposition of a protein digesting enzyme (trypsin).

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Why is it important?

It is important because it is a comprehensive, step-by-step guide, with detailed considerations of each experimental step, including the identification strategy, which matches the imaging data with classical proteomics (shot-gun liquid chromatography coupled to mass spectrometry).