What is it about?
This paper shows that the popular parameter, ratio of fluorescence intensities at two different wavelengths, used in analysis of conformational transitions in proteins should be used with caution. We show that its use may lead to determination of incorrect values of thermodynamic parameters, which characterize the studied transitions, and thus to false conclusions regarding stability of studied proteins (biomacromolecules).
Featured Image
Why is it important?
Using of this parameter may lead to a false hit identification during high-throughput drug screening campaigns, which identify a hit based on the shift of the melting temperature of protein target.
Perspectives
One should be aware of this flaw of using this parameter in protein stability studies and make proper additional experiments and/or corrections in accordance with simple simulations shown in the paper.
Erik Sedlak
Univerzita Pavla Jozefa Stafarika v Kosiciach
Read the Original
This page is a summary of: The fluorescence intensities ratio is not a reliable parameter for evaluation of protein unfolding transitions, Protein Science, April 2017, Wiley,
DOI: 10.1002/pro.3170.
You can read the full text:
Contributors
The following have contributed to this page
