What is it about?
A direct HPTLC assay was developed for the determination of total curcuminoids and three individual curcuminoids, curcumin, demethoxycurcumin and bisdemethoxycurcumin. In addition, a new procedure was developed to separate and quantitative the free radical-scavenging activity of individual compounds from the rhizome of Curcuma longa L. (Zingiberaceae) based on the combination of HPTLC with a diode array detector (DAD) and post chromatographic DPPH• radical derivatisation. It was established that both individual curcuminoids and the extract of C. longa were capable of scavenging DPPH• radicals. From the estimated ID50 values, it can be seen that the order of activity was curcumin > demethoxycurcumin > bisdemethoxycurcumin >> ascorbic acid. However, the ID50 values of curcuminoids were not significantly different.
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Why is it important?
The data indicates the presence of a synergistic mechanism of antiradical activity of curcuminoids.
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This page is a summary of: Separation and free radical‐scavenging activity of major curcuminoids of Curcuma longa using HPTLC‐DPPH method, Phytochemical Analysis, October 2007, Wiley,
DOI: 10.1002/pca.1035.
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