What is it about?
Diffusion ordered spectroscopy (DOSY) is used to determine the translational diffusion coefficients of molecules in solution. However, DOSY is highly susceptible to spurious spectral peaks resulting from thermal convection occurring in the NMR tube. Thermal convection therefore must be suppressed for accurate estimation of translational diffusion coefficients.
Featured Image
Why is it important?
In this study, we developed a new method to effectively suppress thermal convection using glass capillaries. A total of 6 to 18 capillaries (0.8-mm outer diameter) were inserted into a regular 5-mm NMR tube. The capillaries had minimal effect on magnetic field homogeneity and enabled us to obtain clean DOSY spectra of a mixture of small organic compounds. Moreover, the capillaries did not affect chemical shifts or signal intensities in 2-dimensional heteronuclear single quantum coherence spectra. Capillaries are a simple and inexpensive means of suppressing thermal convection and thus can be used in a wide variety of DOSY experiments.
Perspectives
The researcher need not care about thermal convection by using capillary method.
Dr. Takashi Iwashita
Suntory Foundation for Life Sciences
Read the Original
This page is a summary of: Use of glass capillaries to suppress thermal convection in NMR tubes in diffusion measurements, Magnetic Resonance in Chemistry, April 2016, Wiley,
DOI: 10.1002/mrc.4437.
You can read the full text:
Contributors
The following have contributed to this page
