Identifying 15N-13C connectivity networks using covariance processing techniques of NMR data

What is it about?

We report on the utilization of unsymmetrical indirect covariance NMR methods for the combination of 1H-13C GHSQC and 1H-15N long-range heteronuclear chemical shift correlation spectra to determine 15N-13C correlation pathways.

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Why is it important?

It is almost impossible to measure C13-N15 2D NMR spectra directly using NMR experimental approaches. Rather it is possible to measure specific types of 2D NMR spectra (1H-13C GHSQC and 1H-15N long-range heteronuclear chemical shift 2D) and then combine them to generate this type of data using mathematical processing techniques.