Super-resolution, deep imaging with GB-STED
What is it about?
Although STED super-resolution provides new level of insight to biology, it is limited to 2-D due to the scattering of the specimen. By modulating the STED beam to a hollow Bessel beam, we demonstrate 3D deep imaging with Gaussian-Bessel STED (GB-STED), reaching 150 um depth with constant super-resolution.
Why is it important?
Life takes the form of 3D. Yet, despite of previous super-resolution microscopy providing us more indepth understanding to the subcellular activities, it is often limited in 2D, with shallow imaging depth. Our GB-STED can yield deep 3D super-resolution for specimen with large scattering such as brain, enabling the super-resolution in tissue level.
The following have contributed to this page: Dr Peng Xi and Xusan Yang
In partnership with: