What is it about?
Although STED super-resolution provides new level of insight to biology, it is limited to 2-D due to the scattering of the specimen. By modulating the STED beam to a hollow Bessel beam, we demonstrate 3D deep imaging with Gaussian-Bessel STED (GB-STED), reaching 150 um depth with constant super-resolution.
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Why is it important?
Life takes the form of 3D. Yet, despite of previous super-resolution microscopy providing us more indepth understanding to the subcellular activities, it is often limited in 2D, with shallow imaging depth. Our GB-STED can yield deep 3D super-resolution for specimen with large scattering such as brain, enabling the super-resolution in tissue level.
Perspectives
By using the self-healing capability of Bessel beam in STED, we have realized deep 3-D STED super-resolution, on specimen with large refractive index mismatch, and high scattering coefficient. Comparing with the adjustment of correction collar for deep 3D imaging, GB-STED is much faster, without human intervene, and itmaintains its super-resolution for much deeper imaging.
Dr Peng Xi
Peking University
Read the Original
This page is a summary of: Super-resolution deep imaging with hollow Bessel beam STED microscopy, Laser & Photonics Review, November 2015, Wiley,
DOI: 10.1002/lpor.201500151.
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