What is it about?
Analytical instruments with the ability to provide extremely high sensitivities and separation efficiencies are important for both applied sciences and basic research. In capillary electrophoresis with a toroidal platform the analytes perform continuous round trips inside a fused silica capillary with a torus like shape. In the present work the equations of the number of theoretical plates, number of theoretical plates delivered per unit time and peak capacity are deduced when on-column cyclic stacking is used. They are expressed as a function of the number of turns performed by the analyte, number of reservoirs, axial length of the toroid, applied voltage, number of on-column stacking events performed per turn and stacking efficiency.
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Why is it important?
It was found that the variances reach a steady state. The number of theoretical plates grows indefinitely as the square of time, as a consequence. The number of theoretical plates delivered per unit time is also significantly higher if compared to any other separation technique. The expression of peak capacity shows a well-defined limiting value.
Perspectives
it is predicted that a well defined number of peaks can run indefinitely, as peak capacity reaches a constant value when n>>1. This property, together with the predicted unlimited parabolic growth of the number of theoretical plates, has the potential for interesting applications in both applied sciences (to run hard to separate mixtures) and basic research (to study the structure of water itself).
tarso kist
Federal University of Rio Grande do Sul
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This page is a summary of: Cyclic band compression in toroidal capillary electrophoresis delivers an unlimited number of theoretical plates with a quadratic growth in time and a constant peak capacity, Journal of Separation Science, May 2018, Wiley,
DOI: 10.1002/jssc.201800099.
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