pDNA capture using grafted adsorbents

Naveen K Singh, Roy N Dsouza, Vikas Yelemane, Nina Nentwig, Mariano Grasselli, Marcelo Fernández-Lahore
  • Journal of Chemical Technology & Biotechnology, June 2018, Wiley
  • DOI: 10.1002/jctb.5671

pDNA Capture Using Grafted Adsorbents

What is it about?

Composite adsorbents were prepared via either chemical (CG‐DEAE‐NW) or gamma‐irradiated graft‐polymerization (GIR‐DEAE‐MP), and subsequently modified to have diethylamino ethanol (DEAE) functionality. Capture experiments showed that pDNA can actually reversibly bind to the two mentioned adsorbents, with capacities values of 2.4 mg per mL and 1.3 mg per mL, respectively.

Why is it important?

“Expanded” composite materials are of interest as an alternative, or as a supplement, to packed‐bed chromatography during bioproduct recovery and purification. Functionalized non‐woven fabrics and mega‐porous bodies are examples of systems that showed promise. However, there is scarce information on their suitability to capture and release plasmid DNA (pDNA), an important type of product employed in gene therapy.

Perspectives

Dr Naveen Kumar Singh
University of Notre Dame

The values reported for expanded composite adsorbents in this research paper are similar to the values expected from commercial beaded adsorbents but are lower compared to the values expected from monoliths. However, such materials can bind proteins and other contaminants from the bacterial lysate, opening the way for their utilization in the “negative” mode.

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http://dx.doi.org/10.1002/jctb.5671

The following have contributed to this page: Dr Naveen Kumar Singh

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