What is it about?
The way of reading the decrease in absorbance of the reagent greatly improves the sensitivity of the method, as can be deduced from the study of the absorbance spectra. It does not matter that the absorbance [Abs] of the DMMB reagent is greater than the Abs peak of the DMMB-GAG complex at its peak, when measuring the decrease in Abs in the DMMB Abs peak and not the increase in Abs in the maximum of the DMMB‐GAG. The difference of Abs in the maximum of the DMMB and that of the DMMB‐GAG complex at that point is greater than the difference in the maximum of Abs of the DMMB‐GAG complex and that of the DMMB at that other point of the spectrum; with a high concentration of DMMB the latter is unfeasible. Ver: file:///I:/COMMENTSONTHEWORKSRELATEDTOTHEEARLYDETECTIONOFMUCOPOLISACCHARIDOSISINGALICIA--%20(1).pdf
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Why is it important?
The way in which absorbance readings are performed allows the reagent/sample ratio to be multiplied by higher numbers than what was done with this reagent in previous procedures, making it possible to measure concentrations over a wide range.
Perspectives
The method is currently being used in a spectrophotometer that allowed the wavelengths to be optimized and the reagent concentration to be reduced somewhat to avoid excessively high absorbances. The elution time was increased, which can be reduced by using ultrasound. It can be used with liquid urine by making the appropriate adaptations, taking advantage of the way absorbances are read.
Professor José Ramón Alonso-Fernández
Universidade de Santiago de Compostela
Read the Original
This page is a summary of: Neonatal screening for mucopolysaccharidoses by determination of glycosaminoglycans in the eluate of urine-impregnated paper: preliminary results of an improved DMB-based procedure, Journal of Clinical Laboratory Analysis, January 2010, Wiley,
DOI: 10.1002/jcla.20375.
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