What is it about?
Gene targeting in embryonic stem (ES) cells remains best practice for generating knockout and knock-in mice. The traditional method involves generating a chimeric mouse through gene targeting in ES cells. When mated, a percentage of the chimeric offspring are from the modified ES cells that carry the genetic modification. However, this also inevitably produces mice that are not ES cell derived and therefore unnecessary. goGermline technology addresses this issue by generating only the ES cell derived offspring, with no unnecessary mice being produced.
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Why is it important?
goGermline has a major positive impact on animal welfare within knockout and knock-in mouse model production. It improves the 3R’s (Replace, Reduce, Refine) of humane experimental technique with animals. goGermline also makes the knockout and knock-in mouse generation more efficient, thereby reducing project timelines.
Perspectives
Essentially the goGermline technology is a very simple way to make a significant difference to animal welfare. Long live Arthur Schopenhauer, German philosopher (1788 – 1860): "All truth passes through three stages. First, it is ridiculed. Second, it is violently opposed. Third, it is accepted as being self-evident."
Dr Frank Koentgen
Ozgene Pty Ltd
Read the Original
This page is a summary of: Exclusive transmission of the embryonic stem cell‐derived genome through the mouse germline, genesis, May 2016, Wiley,
DOI: 10.1002/dvg.22938.
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