What is it about?
The detection of CD326 positive, CD45 negative, CD33 negative cells is strongly indicative of the presence of carcinoma cells and can be detected in different specimens, by immunophenotyping using flow cytometry.
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Why is it important?
We demonstrated that the detection of positive expression of a single marker, CD326, raises strong suspicion of malignancy and therefore it was not necessary to use a larger panel, as it would increase the cost of the test and would be more time consuming.
Perspectives
This method has extremely high sensitivity and specificity for the evaluation of carcinoma metastasis in all types of specimens that usually lack epithelial cells. The detection of CD326 by flow cytometry represents a complementary test and as such all specimens should also be carefully evaluated morphologically to prevent misdiagnosing; neverthless we have demonstrated that it increases the overall diagnostic accuracy.
Maria João Abrantes Vaz Pais de Amaral Acosta
Hospital S. Francisco Xaviaer
Read the Original
This page is a summary of: Screening of carcinoma metastasis by flow cytometry: A study of 238 cases, Cytometry Part B Clinical Cytometry, July 2015, Wiley,
DOI: 10.1002/cyto.b.21258.
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