What is it about?
Using calculations we show that an intrinsic molecular property (called "negative hyperconjugation") governs the oxidation chemistry of L-lactate and may help to determine its enzymatic dehydrogenation mechanism.
Why is it important?
Flavoenzymes are a class of enzymes implicated in fundamental reactions of cell metabolism. How do these enzymes work? The question is still open for the oxidation of L-lactate in flavocytochrome-b2. We found that the oxidation mechanism of L-lactate in this enzyme is governed by a specific property of the substrate. We found this property to be also present in the substrates of other flavoenzymes, for which the mechanism was known. This validates and generalizes our findings. Hence, calculations on substrate molecules can give us precious information on the inner workings of an important class of enzymes.
Read the Original
This page is a summary of: Does Negative Hyperconjugation Assist Enzymatic Dehydrogenations?, ChemPhysChem, June 2007, Wiley, DOI: 10.1002/cphc.200700085.
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Our article page at Academia (full text of the paper)
Our article page in ResearchGate (full text of the paper)
The oxidation reaction of L-lactate
This is a follow-up work revealing the oxidation mechanism of the substrate in the flavoenzyme active site. The results further confirm our hypotheses on the key role of the substrate's intrinsic properties.
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