What is it about?
Funalia trogii was used for the first time for the removal of bisphenol A (BPA), a well known endocrine disrupting compound. Biodegradation efficiencies of commercial pure laccase from Trametes versicolor, crude extract of F. trogii and the mixture of pure laccase from T. versicolor, and thermally inactivated crude extract of F. trogii were compared. BPA was completely removed by both the crude extract and with mixture whereas only 30% was removed with pure laccase. The results showed the presence of mediator molecules in the crude extract of F. trogii and also confirm the role of laccase in BPA biodegradation. A mediator molecule, butylhydroxytoluene, was detected by GC-MS analysis of the crude extract of F. trogii. The results also proved unnecessity of enzyme isolation procedures for the enzymatic biodegradation of BPA. The BPA concentration was analyzed by HPLC and metabolites of BPA were characterized by GC-MS. The results of both analyses were correlated and BPA was completely removed after 2 h of incubation time. The reaction maximum velocity and Km values obtained from Lineweaver–Burk plots of Michaelis–Menten equation were 7.43mg L1 min1 and 66.35 mg/L, respectively. The acute toxicity of BPA and its products after 6 h incubation period was 98.91.0 and 7.50.4%, respectively. The results of this study point out the use of an alternative feasible method for the complete removal of BPA from polluted environments.
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Why is it important?
The results of this study point out the use of an alternative feasible method for the complete removal of BPA from polluted environments.
Perspectives
This study offers a natural, easily applicable, and economical alternative solution for BPA removal. The GC-MS data of the crude extract showed the presence of a low molecular weight, reducing agent molecule, butylhydroxytoluene. This led to the result that mediator or activator molecules in the crude extract act jointly with the laccase enzyme to degrade BPA. A method that eliminates many difficulties involved in enzyme isolation and an easy and cost-effective procedure was introduced.
Hatice Erkurt
Uluslararasi Kibris Universitesi
Read the Original
This page is a summary of: Biodegradation and Detoxification of BPA: Involving Laccase and a Mediator, CLEAN - Soil Air Water, February 2015, Wiley,
DOI: 10.1002/clen.201400628.
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