What is it about?
Tyrosinase is broadly distributed enzyme. In this study, I engineered a bacterial tyrosinase with circular permutation method. And quantitative production of 3'-hydroxy-genistein was achieved with a tyrosinase variant.
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Why is it important?
This is the first report to apply circular permutation to engineering tyrosinase. A tyrosinase variant enabled efficient preparation of orobol.
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This page is a summary of: Circular permutation of a bacterial tyrosinase enables efficient polyphenol-specific oxidation and quantitative preparation of orobol, July 2018, Wiley,
DOI: 10.1002/bit.26795.
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