What is it about?
By introducing thiamine regulation strategy, thiamine-deficient mutants were selected, and the relationship between thiamine content and cell quantitative accumulation was analyzed. Finally, the efficient synthesis of D-lactic acid under orderly control of cell growth was realized.
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Why is it important?
The contradiction between product synthesis and cell proliferation has been effectively solved, which enables highly active cells to exert the catalytic properties of immobilized enzymes in a special way, thus achieving high speed and high conversion of catalytic process.
Perspectives
The application fields of enzymes were greatly expanded, and the application efficiency of enzymes was greatly improved. The rational combination of cell factories and enzyme engineering was realized organically. This strategy will play an important role in the field of bulk chemical synthesis and enzymatic preparation.
Dr Kangming Tian
Tianjin University of Science and Technology
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This page is a summary of: Limitation of thiamine pyrophosphate supply to growingEscherichia coliswitches metabolism to efficientd-lactate formation, Biotechnology and Bioengineering, August 2015, Wiley,
DOI: 10.1002/bit.25699.
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