Dimerization of Proline Dehydrogenase from Thermus thermophilus Is Crucial for Its Thermostability

Mieke M. E. Huijbers, Jenny W. Wu, Adrie H. Westphal, Willem J. H. Berkel
  • Biotechnology Journal, April 2019, Wiley
  • DOI: 10.1002/biot.201800540

Dimerisation increases the thermal stability of proline dehydrogenase.

What is it about?

Site-directed mutagenesis shows that salt bridges in the dimer interface of proline dehydrogenase from Thermus thermophilus improve the enzyme thermal stability.

Why is it important?

This work learns us more about the molecular determinants of enzyme stability.

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http://dx.doi.org/10.1002/biot.201800540

The following have contributed to this page: Professor Willem J.H. van Berkel