What is it about?
unn pest, Eurygaster integriceps, is a serious pest of cereals in the wide area of the globe from Near and Middle East to East and South Europe and North Africa. This study described for the first time, identification of E. integriceps trypsin serine protease and cathepsin-L cysteine, transcripts involved in digestion, which might serve as targets for pest control management. A total of 478 and 500 base pair long putative trypsin and cysteine gene sequences were characterized and named Tryp and Cys, respectively. In addition, the tissue-specific relative gene expression levels of these genes as well as gluten hydrolase (Gl) were determined under different host kernels feeding conditions. Also possibility of Sunn pest gene silencing was studied by topical application of cysteine double-stranded RNA (dsRNA).
Featured Image
Why is it important?
The results indicated that topically applied dsRNA on fifth nymphal stage can penetrate the cuticle of the insect and induce RNA interference. The Cys gene mRNA transcript in the gut was reduced to 83.8% 2 days posttreatment. Also, it was found that dsRNA of Cys gene affected fifth nymphal stage development suggesting the involvement of this protease in the insect growth, development, and molting.
Perspectives
The objectives of this research were as follows: (1) molecular identification of serine and cysteine protease genes in the Sunn pest genome; (2) study of alterations in expression of salivary glands and gut protease genes in response to different host plants; and (3) silencing cysteine protease gene by using RNAi
Dr Azam Amiri
University of Tehran
Read the Original
This page is a summary of: MOLECULAR IDENTIFICATION OF CYSTEINE AND TRYPSIN PROTEASE, EFFECT OF DIFFERENT HOSTS ON PROTEASE EXPRESSION, AND RNAI MEDIATED SILENCING OF CYSTEINE PROTEASE GENE IN THE SUNN PEST, Archives of Insect Biochemistry and Physiology, November 2015, Wiley,
DOI: 10.1002/arch.21311.
You can read the full text:
Contributors
The following have contributed to this page
