Pronuclei NPB Migration Affects IVF Embryo Ploidy and Live Birth

What is it about?

This content discusses nucleolus precursor bodies (NPBs) and their role in embryonic development after fertilization. NPBs are membraneless organelles that play a crucial role in gene regulation, particularly during the early stages of zygote development. The study investigated the relationship between NPB migration speed, ploidy status, and clinical outcomes in IVF-derived zygotes to determine if differences in the fertilization process affect NPB migration and the relationship between NPB migration, ploidy, and live birth (LB) in IVF-derived zygotes. The study analyzed 18 euploid and 19 aneuploid zygotes from 24 IVF cycles between February 2020 and October 2021. The mean (±SD) age of the women was 38.5 ± 4.2 years when ovum pickup (OPU) was performed (range 29-43 years). The study used a long, short, or GnRH antagonist protocol for controlled ovarian stimulation, and time-lapse microscopy was used to measure NPB migration speed. The study found that NPB migration speed was significantly lower in aneuploid zygotes than in euploid zygotes. Additionally, NPB migration speed was not influenced by fertilization method, and there was no significant correlation between NPB migration speed and embryo morphology. However, a significant correlation was observed between NPB migration speed and ploidy status. The study suggests that NPB migration speed may be a useful marker for predicting the ploidy status of IVF-derived zygotes and could potentially improve the selection of competent embryos for transfer.

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Why is it important?

This research is important because it investigates the relationship between nucleolus precursor body (NPB) migration speed, ploidy status, and clinical outcomes in in vitro fertilization (IVF)-derived zygotes. Understanding these relationships can help clarify the impact of fertilization procedures on NPB migration and predict the likelihood of live births (LBs) in IVF-derived zygotes. This information can contribute to the optimization of assisted reproductive technology (ART) and improve the success rates of IVF and intracytoplasmic sperm injection (ICSI) procedures. Key Takeaways: 1. Nuclear bodies, called nucleolus precursor bodies (NPBs), play a crucial role in gene regulation and are essential for embryonic development after fertilization. 2. NPBs are distinct from nucleoli in somatic cells and have specific morphological features in mammalian oocytes and zygotes. 3. NPB migration speed in intracytoplasmic sperm injection (ICSI)-derived human zygotes predicts the competence of the embryo to develop to the blastocyst stage and result in a live birth (LB). 4. Differences between in vitro fertilization (IVF) and ICSI during the fertilization process may affect NPB migration speed or the ability of NPB migration speed to predict LB. 5. The study investigated the relationship between NPB migration speed, ploidy status, and clinical outcomes after assisted reproductive technology (ART) in IVF-derived zygotes to determine if differences in the fertilization procedure affect NPB migration and clarify the relationship between NPB migration, ploidy, and LB in IVF-derived zygotes. 6. The research emphasizes the importance of understanding the impact of fertilization procedures on NPB migration and predicting the likelihood of live births (LBs) in IVF-derived zygotes to optimize assisted reproductive technology (ART) and improve the success rates of IVF and ICSI procedures.