Phenotypic assays for analyses of pluripotent stem cell-derived cardiomyocytes

  • Martin Pesl, Jan Pribyl, Guido Caluori, Vratislav Cmiel, Ivana Acimovic, Sarka Jelinkova, Petr Dvorak, Zdenek Starek, Petr Skladal, Vladimir Rotrekl
  • Journal of Molecular Recognition, December 2016, Wiley
  • DOI: 10.1002/jmr.2602

Phenotype study of hPSC-derived CMs

What is it about?

Understanding of the heart disfunctions at the cellular level is the paramount step to achieve regeneration of cardiac functionality. Cardiac muscle can be obtained by healthy or diseased pluripotent stem cells, although they require precise functional characterization to be elected for animal and human use. In this work we review methods and propose cutting-edge micro-nanotechnologies to achieve robust characterization

Why is it important?

Evaluation of the genetics and morphology is not sufficient to guarantee cardiac functionality. Phenotypic assays require precise stimuli response, yet most powerful techniques are low-throughput or destructive. Introduction and validation of cell/tissue-level methods of investigation could increase throughput and validity of assays.

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http://dx.doi.org/10.1002/jmr.2602

The following have contributed to this page: Dr. Guido Caluori

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