pDNA Capture Using Grafted Adsorbents
What is it about?
Composite adsorbents were prepared via either chemical (CG‐DEAE‐NW) or gamma‐irradiated graft‐polymerization (GIR‐DEAE‐MP), and subsequently modified to have diethylamino ethanol (DEAE) functionality. Capture experiments showed that pDNA can actually reversibly bind to the two mentioned adsorbents, with capacities values of 2.4 mg per mL and 1.3 mg per mL, respectively.
Why is it important?
“Expanded” composite materials are of interest as an alternative, or as a supplement, to packed‐bed chromatography during bioproduct recovery and purification. Functionalized non‐woven fabrics and mega‐porous bodies are examples of systems that showed promise. However, there is scarce information on their suitability to capture and release plasmid DNA (pDNA), an important type of product employed in gene therapy.
The following have contributed to this page: Dr Naveen Kumar Singh
In partnership with: