What is it about?

Orbitally shaken flasks in polysaccharide-producing bacterial cultures usually present oxygen limitations because of non-Newtonian rheological characteristics of the resulting culture broth and bacterial respiration.

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Why is it important?

by resonant acoustic mixing can maintain elevated oxygen transfer rates, even in non-Newtonian high-viscosity cultures, as in the case of alginate production by A. vinelandii.


RAM showed its oxygen transfer potential for cultures that become increasingly viscous during cultivation, allowing A. vinelandii to have a high accumulation of alginate, without any observable oxygen limitation.

Mauricio A. Trujillo-Roldan
Universidad Nacional Autonoma de Mexico

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This page is a summary of: Shaken flasks by resonant acoustic mixing in the biosynthesis of alginate by Azotobacter vinelandii with non-Newtonian rheological characteristics, Journal of Chemical Technology & Biotechnology, November 2017, Wiley,
DOI: 10.1002/jctb.5475.
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