What is it about?

By adopting the polarization phase shifting and space division multiplexing in digital holographic microscopy, the authors report how they have developed a reflectance quantitative phase microscopy system capable of dynamic quantitative phase imaging and depth-resolved imaging. In addition, the compact design of on-axis near common-path Fizeau polarization interferometry with a wire grid polarizer and the polarization camera offers a single-shot microscopy system with robust phase stability in comparison to other techniques.

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Why is it important?

The integration of a Fizeau-based phase-shifting scheme to the optical microscopy enables a simple and robust tool for the investigations of engineered and biological specimen with real-time quantitative analysis. The high-speed imaging capability with instantaneous recording of spatially phase shifted holograms offers the flexible utilization of the approach in dynamic quantitative phase imaging with robust phase stability. Moreover, the proposed system with high-speed label-free and depth-selective imaging features will be a prospective tool in the quantified investigations in medical diagnosis and in vivo imaging in optical phase microscopy.


We introduce an on-axis phase-shifting digital holography based quantitative phase microscopy system with single-shot imaging capability. The compact design of the system with reflection scheme facilitates the biological imaging of the specimen that would be challenging in the transmission mode, and thereby provides a depth-resolved reconstruction of the phase map of the specimen.

Dr. Vinu R.V
Huaqiao University

Read the Original

This page is a summary of: Label‐free single‐shot imaging with on‐axis phase‐shifting holographic reflectance quantitative phase microscopy, Journal of Biophotonics, March 2022, Wiley, DOI: 10.1002/jbio.202100400.
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