Alaaeldin Hamza
Cisplatin (CIS) provides oxidative stress and inflammations in testicular tissues. Fenugreek seed extract (FSE) is a widely used herbal medicine with potent antioxidant and anti-inflammation properties. The purpose of this study was to investigate the protective effects and the possible mechanisms of FSE against CIS-induced testicular damage in rats. Adult male Wistar rats were given vehicle, single dose of CIS alone (10 mg kg-1), single dose of FSE alone or single dose of CIS followed by FSE (50, 100 or 200 mg kg-1) every day for 5 days. On day 6, oxidative stress and apoptotic testicular toxicity were evaluated.
FSE attenuated both germ cell degenerations and apoptosis in seminiferous tubules in CIS-treated rats. Furthermore, FSE counteracted CIS-induced oxidative stress in rats as assessed by the restoration of superoxide dismutase and catalase activities and reduction in the myeloperoxidase activity and malondialdehyde levels in testes. CIS increased expressions of inducible nitric oxide synthase and nuclear factor-kappa B in testicular tissues. Importantly, treatment with FSE at all doses effectively alleviated all of these inflammatory parameters in testes. Based on these results, we concluded that FSE reduces CIS-induced reproductive toxicity in rats by the suppression of testicular oxidative stress, apoptosis and inflammations.
The aim of this study was to evaluate the protective effects of FSE on CIS-induced alterations in sperm characteristics and biochemical changes related to oxidative stress and inflammations in the testes of rats.our study provides experimental evidence that concomitant treatment of FSE with CIS attenuated CIS-induced reproductive toxicity and testicular histopathological changes by antioxidants effects. We believe that protective effects of FSE against CIS reproductive toxicity is mediated primarily through both the inhibition of apoptosis and the abrogation of oxidative stress and iNOS and NF-kB inflammatory response associated with CIS treatment. These observations are of clinical important as FSE could be a promising target to halt this serious testicular toxicity induced by CIS.
National Organization for Drug Control and Research
our study provides experimental evidence that concomitant treatment of FSE with CIS attenuated CIS-induced reproductive toxicity and testicular histopathologica
