All Stories

  1. “Specificity Determinants” Improve Therapeutic Indices of Two Antimicrobial Peptides Piscidin 1 and Dermaseptin S4 Against the Gram-negative Pathogens Acinetobacter baumannii and Pseudomonas aeruginosa

    Article • Pharmaceuticals, March 2014, MDPI AG

    Michael Vasil

  2. The complex interplay of iron, biofilm formation, and mucoidy affecting antimicrobial resistance of Pseudomonas aeruginosa

    Article • Pathogens and Disease, February 2014, Oxford University Press (OUP)

    Michael Vasil, Amanda Oglesby-Sherrouse

  3. Iron-Regulated Expression of Alginate Production, Mucoid Phenotype, and Biofilm Formation by Pseudomonas aeruginosa

    Article • mBio, February 2014, ASM Journals

    Michael Vasil, Dr Michael J Schurr

  4. High-level over-expression, purification, and crystallization of a novel phospholipase C/sphingomyelinase from Pseudomonas aeruginosa

    Article • Protein Expression and Purification, July 2013, Elsevier

    Michael Vasil

  5. Recruitment of a phospholipase C/sphingomyelinase into non-lamellar lipid droplets during hydrolysis of lipid bilayers

    Article • Chemistry and Physics of Lipids, January 2013, Elsevier

    Michael Vasil

  6. Identification and Evaluation of Twin-Arginine Translocase Inhibitors

    Article • Antimicrobial Agents and Chemotherapy, September 2012, ASM Journals

    Michael Vasil

  7. Neutrophil Extracellular Trap (NET)-Mediated Killing of Pseudomonas aeruginosa: Evidence of Acquired Resistance within the CF Airway, Independent of CFTR

    Article • PLoS ONE, September 2011, PLOS

    Michael Vasil, Ken Malcolm

  8. The Lipase LipA (PA2862) but Not LipC (PA4813) from Pseudomonas aeruginosa Influences Regulation of Pyoverdine Production and Expression of the Sigma Factor PvdS

    Article • Journal of Bacteriology, August 2011, ASM Journals

    Michael Vasil

  9. Hemolytic Phospholipase C Inhibition Protects Lung Function duringPseudomonas aeruginosaInfection

    Article • American Journal of Respiratory and Critical Care Medicine, August 2011, American Thoracic Society

    Michael Vasil, Deborah A Hogan

  10. Specificity determinants dramatically reduce hemolytic activity in amphipathic α-helical antimicrobial peptides: antimicrobial activity against Gram-negative pathogens,Acinetobacter baumanniiandPseudomonas aeruginosa

    Article • July 2011, World Scientific Pub Co Pte Lt

    Michael Vasil

  11. Rational Design of α‐Helical Antimicrobial Peptides to Target Gram‐negative Pathogens, Acinetobacter baumannii and Pseudomonas aeruginosa: Utilization of Charge, ‘Specificity Determinants,’ Total Hydrophobicity, Hydrophobe Type and Locati...

    Article • Chemical Biology & Drug Design, February 2011, Wiley

    Michael Vasil

  12. Imaging the early stages of phospholipase C/sphingomyelinase activity on vesicles containing coexisting ordered-disordered and gel-fluid domains

    Article • Journal of Lipid Research, January 2011, American Society for Biochemistry & Molecular Biology (ASBMB)

    Michael Vasil

  13. Multiple phospholipid substrates of phospholipase C/sphingomyelinase HR2 from Pseudomonas aeruginosa

    Article • Chemistry and Physics of Lipids, January 2011, Elsevier

    Michael Vasil

  14. Molecular Investigations of PenA-mediated β-lactam Resistance in Burkholderia pseudomallei

    Article • Frontiers in Microbiology, January 2011, Frontiers

    Michael Vasil

  15. Characterization of a Heme-Regulated Non-Coding RNA Encoded by the prrF Locus of Pseudomonas aeruginosa

    Article • PLoS ONE, April 2010, PLOS

    Michael Vasil, Amanda Oglesby-Sherrouse

  16. End-products diacylglycerol and ceramide modulate membrane fusion induced by a phospholipase C/sphingomyelinase from Pseudomonas aeruginosa

    Article • Biochimica et Biophysica Acta (BBA) - Biomembranes, January 2010, Elsevier

    Michael Vasil

  17. A Complex Extracellular Sphingomyelinase of Pseudomonas aeruginosa Inhibits Angiogenesis by Selective Cytotoxicity to Endothelial Cells

    Article • PLoS Pathogens, May 2009, PLOS

    Michael Vasil

  18. Neutrophil enhancement of Pseudomonas aeruginosa biofilm development: human F-actin and DNA as targets for therapy

    Article • Journal of Medical Microbiology, April 2009, Society for General Microbiology

    Michael Vasil

  19. Ceramide-Enriched Membrane Domains in Red Blood Cells and the Mechanism of Sphingomyelinase-Induced Hot-Cold Hemolysis

    Article • Biophysical Journal, February 2009, Elsevier

    Michael Vasil

  20. Mycobacterium tuberculosis Rv3802c Encodes a Phospholipase/Thioesterase and Is Inhibited by the Antimycobacterial Agent Tetrahydrolipstatin

    Article • PLoS ONE, January 2009, PLOS

    Michael Vasil

  21. Effects of net charge and the number of positively charged residues on the biological activity of amphipathic α-helical cationic antimicrobial peptides

    Article • January 2009, Springer Science + Business Media

    Michael Vasil, Dr Robert E Hancock

  22. Effects of Hydrophobicity on the Antifungal Activity of α‐Helical Antimicrobial Peptides

    Article • Chemical Biology & Drug Design, November 2008, Wiley

    Michael Vasil, Dr Robert E Hancock

  23. Ceramide-Enriched Membrane Domains in Red Blood Cells and the Mechanism of Sphingomyelinase-Induced Hot−Cold Hemolysis

    Article • Biochemistry, October 2008, American Chemical Society (ACS)

    Michael Vasil

  24. The Metal-Dependent Regulators FurA and FurB from Mycobacterium Tuberculosis

    Article • International Journal of Molecular Sciences, August 2008, MDPI AG

    Michael Vasil

  25. The Influence of Iron on Pseudomonas aeruginosa Physiology: A REGULATORY LINK BETWEEN IRON AND QUORUM SENSING

    Article • Journal of Biological Chemistry, April 2008, American Society for Biochemistry & Molecular Biology (ASBMB)

    Michael Vasil, Amanda Oglesby-Sherrouse

  26. Pseudomonas aeruginosa Twitching Motility-Mediated Chemotaxis towards Phospholipids and Fatty Acids: Specificity and Metabolic Requirements

    Article • Journal of Bacteriology, April 2008, ASM Journals

    Michael Vasil

  27. Effects of net charge and the number of positively charged residues on the biological activity of amphipathic α‐helical cationic antimicrobial peptides

    Article • Biopolymers, January 2008, Wiley

    Michael Vasil, Dr Robert E Hancock

  28. Leakage-free membrane fusion induced by the hydrolytic activity of PlcHR2, a novel phospholipase C/sphingomyelinase from Pseudomonas aeruginosa

    Article • Biochimica et Biophysica Acta (BBA) - Biomembranes, October 2007, Elsevier

    Michael Vasil

  29. Characterization of two distinct phospholipase C enzymes from Burkholderia pseudomallei

    Article • Microbiology, June 2007, Society for General Microbiology

    Michael Vasil

  30. Purification and Characterization of Mycobacterial Phospholipase A: an Activity Associated with Mycobacterial Cutinase

    Article • Journal of Bacteriology, April 2007, ASM Journals

    Michael Vasil

  31. How we learnt about iron acquisition in Pseudomonas aeruginosa: a series of very fortunate events

    Article • BioMetals, December 2006, Springer Science + Business Media

    Michael Vasil

  32. Role of Peptide Hydrophobicity in the Mechanism of Action of α-Helical Antimicrobial Peptides

    Article • Antimicrobial Agents and Chemotherapy, December 2006, ASM Journals

    Michael Vasil

  33. Role of the Pseudomonas aeruginosa PlcH Tat Signal Peptide in Protein Secretion, Transcription, and Cross-Species Tat Secretion System Compatibility

    Article • Journal of Bacteriology, March 2006, ASM Journals

    Michael Vasil

  34. Comparison of Biophysical and Biologic Properties of α‐Helical Enantiomeric Antimicrobial Peptides

    Article • Chemical Biology & Drug Design, February 2006, Wiley

    Michael Vasil

  35. Pseudomonas aeruginosa Phospholipases and Phospholipids

    Article • January 2006, Springer Science + Business Media

    Michael Vasil

  36. Iron and Pseudomonas aeruginosa biofilm formation

    Article • Proceedings of the National Academy of Sciences, July 2005, Proceedings of the National Academy of Sciences

    Michael Vasil

  37. Enhanced Pseudomonas aeruginosa Biofilm Development Mediated by Human Neutrophils

    Article • Infection and Immunity, June 2005, ASM Journals

    Michael Vasil

  38. The Pseudomonas aeruginosa pirA gene encodes a second receptor for ferrienterobactin and synthetic catecholate analogues

    Article • FEMS Microbiology Letters, May 2005, Oxford University Press (OUP)

    Michael Vasil, Pierre Cornelis

  39. Rational Design of  -Helical Antimicrobial Peptides with Enhanced Activities and Specificity/Therapeutic Index

    Article • Journal of Biological Chemistry, January 2005, American Society for Biochemistry & Molecular Biology (ASBMB)

    Michael Vasil

  40. A novel extracellular phospholipase C of Pseudomonas aeruginosa is required for phospholipid chemotaxis

    Article • Molecular Microbiology, July 2004, Wiley

    Michael Vasil

  41. Identification of tandem duplicate regulatory small RNAs in Pseudomonas aeruginosa involved in iron homeostasis

    Article • Proceedings of the National Academy of Sciences, June 2004, Proceedings of the National Academy of Sciences

    Michael Vasil

  42. Purification, Characterization, and Identification of a Sphingomyelin Synthase from Pseudomonas aeruginosa: PlcH IS A MULTIFUNCTIONAL ENZYME

    Article • Journal of Biological Chemistry, June 2003, American Society for Biochemistry & Molecular Biology (ASBMB)

    Michael Vasil

  43. DNA Microarrays in Analysis of Quorum Sensing: Strengths and Limitations

    Article • Journal of Bacteriology, April 2003, ASM Journals

    Michael Vasil

  44. Architecture of a protein central to iron homeostasis: crystal structure and spectroscopic analysis of the ferric uptake regulator

    Article • Molecular Microbiology, February 2003, Wiley

    Michael Vasil

  45. A novel class of microbial phosphocholine‐specific phospholipases C

    Article • Molecular Microbiology, October 2002, Wiley

    Michael Vasil

  46. GeneChip® expression analysis of the iron starvation response in Pseudomonas aeruginosa: identification of novel pyoverdine biosynthesis genes

    Article • Molecular Microbiology, September 2002, Wiley

    Michael Vasil

  47. Pseudomonas aeruginosa Synthesizes Phosphatidylcholine by Use of the Phosphatidylcholine Synthase Pathway

    Article • Journal of Bacteriology, September 2002, ASM Journals

    Michael Vasil

  48. Crystal structure and X-ray absorption spectroscopic analysis of the ferric uptake regulator

    Article • Acta Crystallographica Section A Foundations of Crystallography, August 2002, International Union of Crystallography

    Michael Vasil

  49. Effects of the twin-arginine translocase on secretion of virulence factors, stress response, and pathogenesis

    Article • Proceedings of the National Academy of Sciences, May 2002, Proceedings of the National Academy of Sciences

    Michael Vasil

  50. Siderophore-mediated signaling regulates virulence factor production in Pseudomonas aeruginosa

    Article • Proceedings of the National Academy of Sciences, May 2002, Proceedings of the National Academy of Sciences

    Michael Vasil

  51. Characterization of an Endoprotease (PrpL) Encoded by a PvdS-Regulated Gene in Pseudomonas aeruginosa

    Article • Infection and Immunity, September 2001, ASM Journals

    Michael Vasil

  52. Genetic and Physiological Characterization ofohr, Encoding a Protein Involved in Organic Hydroperoxide Resistance in Pseudomonas aeruginosa

    Article • Journal of Bacteriology, January 2001, ASM Journals

    Michael Vasil

  53. Genetic and biochemical analyses of a eukaryotic‐like phospholipase D of Pseudomonas aeruginosa suggest horizontal acquisition and a role for persistence in a chronic pulmonary infection model

    Article • Molecular Microbiology, January 2001, Wiley

    Michael Vasil

  54. Disruption of the β-Sheet Structure of Cyclic Peptides by Single Amino Acid Substitution: Influence on Prokaryotic and Eukaryotic Cell Viability

    Article • January 2001, Springer Science + Business Media

    Michael Vasil, Dr Robert E Hancock

  55. Role of the Pseudomonas aeruginosa oxyR-recG Operon in Oxidative Stress Defense and DNA Repair: OxyR-Dependent Regulation of katB-ankB, ahpB, andahpC-ahpF

    Article • Journal of Bacteriology, August 2000, ASM Journals

    Michael Vasil

  56. Characterization of the 2‐ketogluconate utilization operon in Pseudomonas aeruginosa PAO1

    Article • Molecular Microbiology, August 2000, Wiley

    Michael Vasil

  57. The Oxygen‐ and Iron‐Dependent Sigma Factor pvdS of Pseudomonas aeruginosa Is an Important Virulence Factor in Experimental Infective Endocarditis

    Article • The Journal of Infectious Diseases, March 2000, Oxford University Press (OUP)

    Michael Vasil

  58. The response of Pseudomonas aeruginosa to iron: genetics, biochemistry and virulence

    Article • Molecular Microbiology, November 1999, Wiley

    Michael Vasil

  59. Role of Haemolytic and Non-Haemolytic Phospholipase C from Pseudomonas Aeruginosa in Interleukin-8 release from Human Monocytes

    Article • Journal of Medical Microbiology, June 1997, Society for General Microbiology

    Michael Vasil

  60. Molecular characterization of mutants affected in the osmoprotectant‐dependent induction of phospholipase C in Pseudomonas aeruginosa PAO1

    Article • Molecular Microbiology, January 1997, Wiley

    Michael Vasil

  61. Role of Hemolytic and Nonhemolytic Phospholipase C from <i>Pseudomonas aeruginos</i><i>a</i> for Inflammatory Mediator Release from Human Granulocytes

    Article • International Archives of Allergy and Immunology, January 1997, Karger Publishers

    Michael Vasil

  62. Linker insertion scanning of regA, an activator of exotoxin A production in Pseudomonas aeruginosa

    Article • Molecular Microbiology, October 1996, Wiley

    Michael Vasil

  63. Ferric uptake regulator mutants of Pseudomonas aeruginosa with distinct alterations in the iron‐dependent repression of exotoxin A and siderophores in aerobic and microaerobic environments

    Article • Molecular Microbiology, September 1996, Wiley

    Michael Vasil

  64. Exotoxin A production in Pseudomonas aeruginosa requires the iron‐regulated pvdS gene encoding an alternative sigma factor

    Article • Molecular Microbiology, September 1996, Wiley

    Michael Vasil

  65. Isolation and characterization of a Pseudomonas aeruginosa gene, ptxR, which positively regulates exotoxin A production

    Article • Molecular Microbiology, July 1996, Wiley

    Michael Vasil

  66. Gene repression by the ferric uptake regulator in Pseudomonas aeruginosa: cycle selection of iron-regulated genes.

    Article • Proceedings of the National Academy of Sciences, April 1996, Proceedings of the National Academy of Sciences

    Michael Vasil

  67. Comparative usefulness of Ribotyping, exotoxin A genotyping, and SalI restriction fragment length polymorphism analysis for Pseudomonas aeruginosa lineage assessment

    Article • Diagnostic Microbiology and Infectious Disease, April 1996, Elsevier

    Michael Vasil

  68. A new extracellular protein of Pseudomonas aeruginosa PA103 regulated by regA

    Article • Microbiology, July 1994, Society for General Microbiology

    Michael Vasil

  69. Osmoprotectants and phosphate regulate expression of phospholipase C in Pseudomonas aeruginosa

    Article • Molecular Microbiology, April 1992, Wiley

    Michael Vasil

  70. Regulation of toxA and regA by the Escherichia coli fur gene and identification of a Fur homologue in Pseudomonas aeruginosa PA103 and PA01

    Article • Molecular Microbiology, November 1991, Wiley

    Michael Vasil

  71. Pseudomonas aeruginosa LasB mutant constructed by insertional mutagenesis reveais elastolytic activity due to alkaline proteinase and the LasA fragment

    Article • Molecular Microbiology, September 1991, Wiley

    Michael Vasil

  72. Decreased Pulmonary Vasoreactivity in an Animal Model of ChronicPseudomonasPneumonia

    Article • American Review of Respiratory Disease, July 1990, American Thoracic Society

    Michael Vasil

  73. Pseudomonas aeruginosacross-colonization and persistence in patients with cystic fibrosis. Use of a DNA probe

    Article • Epidemiology and Infection, April 1989, Cambridge University Press

    Christiane Wolz, Michael Vasil

  74. Regulation of exotoxin A synthesis in Pseudomonas aeruginosa: characterization of toxA‐lacZ fusions in wild‐type and mutant strains

    Article • Molecular Microbiology, March 1989, Wiley

    Michael Vasil

  75. Development of Resistance in Pseudomonas aeruginosa to Imipenem, Norfloxacin, and Ciprofloxacin During Therapy: Proof Provided by Typing with a DNA Probe

    Article • The Journal of Infectious Diseases, April 1988, Oxford University Press (OUP)

    Michael Vasil

  76. Characterization and Use of a DNA Probe as an Epidemiological Marker for Pseudomonas aeruginosa

    Article • The Journal of Infectious Diseases, January 1987, Oxford University Press (OUP)

    Michael Vasil

  77. Pseudomonas aeruginosa: Biology, mechanisms of virulence, epidemiology

    Article • The Journal of Pediatrics, May 1986, Elsevier

    Michael Vasil

  78. Relation between antibody response to pseudomonas aeruginosa exoproteins and colonization/infection in patients with cystic fibrosis. by granström m, ericsson a, strandvik b, wretlind b, pavlovskis or, berka r, and vasil ml. acta paediatr scand ...

    Article • Pediatric Pulmonology, July 1985, Wiley

    Michael Vasil

  79. Enzyme-linked immunosorbent assay for detection of antibodies toPseudomonas aeruginosa exoproteins

    Article • European Journal of Clinical Microbiology & Infectious Diseases, April 1985, Springer Science + Business Media

    Michael Vasil

  80. Relation between Antibody Response to Pseudomonas aeruginosa Exoproteins and Colonization/Infection in Patients with Cystic Fibrosis

    Article • Acta Paediatrica, November 1984, Wiley

    Michael Vasil, Birgitta Strandvik

  81. Cloning, nucleotide sequence, and expression in Escherichia coli of the exotoxin A structural gene of Pseudomonas aeruginosa.

    Article • Proceedings of the National Academy of Sciences, May 1984, Proceedings of the National Academy of Sciences

    Michael Vasil

  82. Mapping of a gene controlling the production of phospholipase C and alkaline phosphatase in Pseudomonas aeruginosa

    Article • October 1981

    Michael Vasil

  83. Regulation of granulopoiesis and distribution of granulocytes in early phase of bacterial infection

    Article • Journal of Cellular Physiology, October 1981, Wiley

    Michael Vasil

  84. The Effect of Bacterial Infection on Granulopoiesis

    Article • Experimental Biology and Medicine, May 1981, Frontiers

    Michael Vasil

  85. Comparative Toxicities of Diphtherial Toxin and Pseudomonas aeruginosa Exotoxin A: Evidence for Different Cell Receptors

    Article • Journal of General Microbiology, October 1978, Society for General Microbiology

    Michael Vasil

  86. Isolation and Properties of Heat-Labile Enterotoxin(s) from Enterotoxigenic Escherichia coli

    Article • The Journal of Infectious Diseases, March 1976, Oxford University Press (OUP)

    Michael Vasil

  87. Conjugal transfer of a chromosomal gene determining production of enterotoxin in vibrio cholerae

    Article • Science, March 1975, American Association for the Advancement of Science

    Michael Vasil

  88. Studies on toxinogenesis in Vibrio cholerae. III. Characterization of nontoxinogenic mutants in vitro and in experimental animals.

    Article • Journal of Clinical Investigation, March 1975, American Society for Clinical Investigation

    Michael Vasil

  89. Studies on Toxinogenesis in Vibrio cholerae. I. Isolation of Mutants with Altered Toxinogenicity

    Article • The Journal of Infectious Diseases, February 1974, Oxford University Press (OUP)

    Michael Vasil

  90. Rational Design of α-Helical Antimicrobial Peptides

    Article • Springer Science + Business Media

    Michael Vasil