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  1. Artificial Self-Sufficient Cytochrome P450 Containing Multiple Auxiliary Proteins Demonstrates Improved Monooxygenase Activity

    Article • Biotechnology Journal, August 2018, Wiley

    Dr Hidehiko Hirakawa

  2. A proteinous tool to assemble multiple proteins

    Article • Biotechnology Journal, April 2018, Wiley

    Dr Hidehiko Hirakawa

  3. Enzyme Logic Gate with Summation Function Based on Putidaredoxin Reductase/Cytochrome c Enzyme Reaction

    Article • Journal of Nanoscience and Nanotechnology, August 2017, American Scientific Publishers

    Dr Hidehiko Hirakawa

  4. Immobilization of a Bacterial Cytochrome P450 Monooxygenase System on a Solid Support

    Article • Angewandte Chemie, October 2016, Wiley

    Dr Hidehiko Hirakawa

  5. Co-immobilization of a bacterial monooxygenase with its auxiliary proteins on magnetic beads

    Article • Angewandte Chemie International Edition, October 2016, Wiley

    Dr Hidehiko Hirakawa

  6. A heterotrimeric proliferating cell nuclear antigen from Metallosphaera sedula

    Article • Scientific Reports, May 2016, Nature

    Dr Hidehiko Hirakawa

  7. Correlation between Activity and Molecular Structure around the Active Center of Cytochrome P450cam Conjugates

    Article • JOURNAL OF CHEMICAL ENGINEERING OF JAPAN, January 2016, Society of Chemical Engineers, Japan

    Dr Hidehiko Hirakawa

  8. Ca2+-independent sortase-A exhibits high selective protein ligation activity in the cytoplasm ofEscherichia coli

    Article • Biotechnology Journal, April 2015, Wiley

    Dr Hidehiko Hirakawa

  9. Supramolecular protein assembly supports immobilization of a cytochrome P450 monooxygenase system as water-insoluble gel

    Article • Scientific Reports, March 2015, Springer Science + Business Media

    Dr Hidehiko Hirakawa

  10. Fusion protein bilayer fabrication composed of recombinant azurin/cytochrome P450 by the sortase-mediated ligation method

    Article • Colloids and Surfaces B Biointerfaces, August 2014, Elsevier

    Dr Hidehiko Hirakawa

  11. Electron donation to an archaeal cytochrome P450 is enhanced by PCNA-mediated selective complex formation with foreign redox proteins

    Article • Biotechnology Journal, July 2014, Wiley

    Dr Hidehiko Hirakawa

  12. Effect of length of molecular recognition moiety on enzymatic activity switching

    Article • Journal of Bioscience and Bioengineering, October 2013, Elsevier

    Dr Hidehiko Hirakawa

  13. Fine Tuning of Spatial Arrangement of Enzymes in a PCNA-Mediated Multienzyme Complex Using a Rigid Poly-L-Proline Linker

    Article • PLoS ONE, September 2013, PLOS

    Dr Hidehiko Hirakawa

  14. Intracellular protein cyclization catalyzed by exogenously transduced Streptococcus pyogenes sortase A

    Article • Journal of Bioscience and Bioengineering, September 2013, Elsevier

    Dr Hidehiko Hirakawa

  15. Phosphite-driven Self-sufficient Cytochrome P450

    Article • ChemCatChem, August 2013, Wiley

    Dr Hidehiko Hirakawa

  16. Molecular recognition moiety and its target biomolecule interact in switching enzyme activity

    Article • Journal of Bioscience and Bioengineering, June 2013, Elsevier

    Dr Hidehiko Hirakawa

  17. Stabilization of heterotrimeric protein complex by disulfide bonds

    Article • Biotechnology and Bioengineering, February 2013, Wiley

    Dr Hidehiko Hirakawa

  18. Use of Sulfolobus solfataricus PCNA Subunit Proteins to Direct the Assembly of Multimeric Enzyme Complexes

    Article • January 2013, Springer Science + Business Media

    Dr Hidehiko Hirakawa

  19. Artificial Protein Complexes for Biocatalysis

    Article • Topics in Catalysis, September 2012, Springer Science + Business Media

    Dr Hidehiko Hirakawa

  20. Design of Ca2+-independentStaphylococcus aureussortase A mutants

    Article • Biotechnology and Bioengineering, July 2012, Wiley

    Dr Hidehiko Hirakawa

  21. Enhancement of sortase A-mediated protein ligation by inducing a β-hairpin structure around the ligation site

    Article • Chemical Communications, January 2011, Royal Society of Chemistry

    Dr Hidehiko Hirakawa

  22. Nanoscale-Engineered Cytochrome P450 System with a Branch Structure

    Article • January 2011, Springer Science + Business Media

    Dr Hidehiko Hirakawa

  23. Molecular Assembly of P450 with Ferredoxin and Ferredoxin Reductase by Fusion to PCNA

    Article • ChemBioChem, July 2010, Wiley

    Dr Hidehiko Hirakawa

  24. Crystal Structure of Histamine Dehydrogenase from Nocardioides simplex

    Article • Journal of Biological Chemistry, June 2010, American Society for Biochemistry & Molecular Biology (ASBMB)

    Dr Hidehiko Hirakawa, Dr Gerald H Lushington

  25. Artificial Self-Sufficient P450 in Reversed Micelles

    Article • Molecules, April 2010, MDPI AG

    Dr Hidehiko Hirakawa

  26. Expression, purification, crystallization and preliminary X-ray studies of histamine dehydrogenase fromNocardioides simplex

    Article • Acta Crystallographica Section F Structural Biology and Crystallization Communications, August 2008, International Union of Crystallography

    Dr Hidehiko Hirakawa

  27. Intramolecular electron transfer in a cytochrome P450cam system with a site-specific branched structure

    Article • Protein Engineering Design and Selection, August 2007, Oxford University Press (OUP)

    Dr Hidehiko Hirakawa

  28. Activity of a thermophilic alcohol dehydrogenase is improved under hydrophobic condition

    Article • Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics, April 2005, Elsevier

    Dr Hidehiko Hirakawa

  29. Properties of an Alcohol Dehydrogenase from the Hyperthermophilic Archaeon Aeropyrum pernix K1

    Article • Journal of Bioscience and Bioengineering, January 2004, Elsevier

    Dr Hidehiko Hirakawa

  30. Properties of an alcohol dehydrogenase from the hyperthermophilic archaeon Aeropyrum pernix K1

    Article • Journal of Bioscience and Bioengineering, January 2004, Elsevier

    Dr Hidehiko Hirakawa

  31. Coupling reaction by steroid dehydrogenase and alcohol dehydrogenase in reversed micellar system

    Article • Biochemical Engineering Journal, October 2003, Elsevier

    Dr Hidehiko Hirakawa

  32. Site-selective and stepwise complexation of two M(cod)+ (M = Rh, Ir) fragments with calix[4]areneElectronic supplementary information (ESI) available: experimental section. Fig. S1: structure of one of the independent molecules of 4. See http://www.rsc...

    Article • Chemical Communications, April 2002, Royal Society of Chemistry

    Dr Hidehiko Hirakawa