All Stories

  1. Using disruptive insertional mutagenesis to identify the in situ structure-function landscape of the Shigella translocator protein IpaB

    Article • Protein Science, May 2018, Wiley

    Professor William D Picking, Meenakumari Muthuramalingam

  2. Biophysical Characterization of the Type III Secretion System Translocator Proteins and the Translocator Proteins Attached to Bacterium-Like Particles

    Article • Journal of Pharmaceutical Sciences, December 2015, Elsevier

    Professor William D Picking

  3. Biophysical Characterization of the Type III Secretion Tip Proteins and the Tip Proteins Attached to Bacterium-Like Particles

    Article • Journal of Pharmaceutical Sciences, February 2015, Elsevier

    Professor William D Picking

  4. Visualization of the type III secretion sorting platform of Shigella flexneri

    Article • Proceedings of the National Academy of Sciences, January 2015, Proceedings of the National Academy of Sciences

    Professor William D Picking

  5. Impact of Detergent on Biophysical Properties and Immune Response of the IpaDB Fusion Protein, a Candidate Subunit Vaccine against Shigella Species

    Article • Infection and Immunity, November 2014, ASM Journals

    Professor William D Picking

  6. Influence of oligomerization state on the structural properties of invasion plasmid antigen B fromShigella flexneriin the presence and absence of phospholipid membranes

    Article • Proteins Structure Function and Bioinformatics, August 2014, Wiley

    Professor William D Picking

  7. The Effect of Fiber Truncations on the Stability of Adenovirus Type 5

    Article • Molecular Biotechnology, July 2014, Springer Science + Business Media

    Professor William D Picking

  8. Experimental salmonellosis challenge model in older calves

    Article • Veterinary Microbiology, May 2014, Elsevier

    Professor William D Picking

  9. N-Terminus of IpaB Provides a Potential Anchor to the Shigella Type III Secretion System Tip Complex Protein IpaD

    Article • Biochemistry, December 2013, American Chemical Society (ACS)

    Professor William D Picking

  10. Characterization of a Novel Fusion Protein from IpaB and IpaD of Shigella spp. and Its Potential as a Pan-Shigella Vaccine

    Article • Infection and Immunity, September 2013, ASM Journals

    Professor William D Picking

  11. A Systematic Approach Toward Stabilization of CagL, a Protein Antigen from Helicobacter Pylori That is a Candidate Subunit Vaccine

    Article • Journal of Pharmaceutical Sciences, August 2013, Elsevier

    Professor William D Picking

  12. Studies of the conformational stability of invasion plasmid antigen B from Shigella

    Article • Protein Science, March 2013, Wiley

    Professor William D Picking

  13. Oligomeric states of the Shigella translocator protein IpaB provide structural insights into formation of the type III secretion translocon

    Article • Protein Science, March 2013, Wiley

    Professor William D Picking

  14. Förster Resonance Energy Transfer (FRET) as a Tool for Dissecting the Molecular Mechanisms for Maturation of the Shigella Type III Secretion Needle Tip Complex

    Article • International Journal of Molecular Sciences, November 2012, MDPI AG

    Professor William D Picking

  15. Binding Affects the Tertiary and Quaternary Structures of the Shigella Translocator Protein IpaB and Its Chaperone IpgC

    Article • Biochemistry, May 2012, American Chemical Society (ACS)

    Professor William D Picking

  16. The Structures of Coiled-Coil Domains from Type III Secretion System Translocators Reveal Homology to Pore-Forming Toxins

    Article • Journal of Molecular Biology, April 2012, Elsevier

    Professor William D Picking

  17. Identification of the bile salt binding site on ipad from Shigella flexneri and the influence of ligand binding on IpaD structure

    Article • Proteins Structure Function and Bioinformatics, December 2011, Wiley

    Professor William D Picking

  18. Conformational Changes in IpaD from Shigella flexneri upon Binding Bile Salts Provide Insight into the Second Step of Type III Secretion

    Article • Biochemistry, January 2011, American Chemical Society (ACS)

    Professor William D Picking

  19. Formulation and Immunogenicity Studies of Type III Secretion System Needle Antigens as Vaccine Candidates

    Article • Journal of Pharmaceutical Sciences, November 2010, Elsevier

    Professor William D Picking

  20. Formulation and Immunogenicity of a Potential Multivalent Type III Secretion System-Based Protein Vaccine

    Article • Journal of Pharmaceutical Sciences, November 2010, Elsevier

    Professor William D Picking

  21. Liposomes Recruit IpaC to the Shigella flexneri Type III Secretion Apparatus Needle as a Final Step in Secretion Induction

    Article • Infection and Immunity, May 2009, ASM Journals

    Professor William D Picking

  22. The C-terminus of IpaC is required for effector activities related to Shigella invasion of host cells

    Article • Microbial Pathogenesis, October 2008, Elsevier

    Professor William D Picking

  23. The response of type three secretion system needle proteins MxiHΔ5 , BsaLΔ5 , and PrgIΔ5 to temperature and pH

    Article • Proteins Structure Function and Bioinformatics, May 2008, Wiley

    Professor William D Picking

  24. Deoxycholate Interacts with IpaD of Shigella flexneri in Inducing the Recruitment of IpaB to the Type III Secretion Apparatus Needle Tip

    Article • Journal of Biological Chemistry, May 2008, American Society for Biochemistry & Molecular Biology (ASBMB)

    Professor William D Picking, Dr Gerald H Lushington

  25. pH sensitivity of type III secretion system tip proteins

    Article • Proteins Structure Function and Bioinformatics, January 2008, Wiley

    Professor William D Picking

  26. Structural dissection of the extracellular moieties of the type III secretion apparatus

    Article • Molecular BioSystems, January 2008, Royal Society of Chemistry

    Professor William D Picking

  27. Evaluating Burkholderia pseudomallei Bip proteins as vaccines and Bip antibodies as detection agents

    Article • FEMS Immunology & Medical Microbiology, January 2008, Oxford University Press (OUP)

    Professor William D Picking

  28. Preparation and Characterization of Translocator/Chaperone Complexes and Their Component Proteins from Shigella flexneri †

    Article • Biochemistry, July 2007, American Chemical Society (ACS)

    Professor William D Picking

  29. Bile Salts Stimulate Recruitment of IpaB to the Shigella flexneri Surface, Where It Colocalizes with IpaD at the Tip of the Type III Secretion Needle

    Article • Infection and Immunity, February 2007, ASM Journals

    Professor William D Picking

  30. SeMet substituted Shigella Flexneri Ipad

    Article • November 2006, Protein Data Bank, Rutgers University

    Professor William D Picking

  31. Expression, limited proteolysis and preliminary crystallographic analysis of IpaD, a component of theShigella flexneritype III secretion system

    Article • Acta Crystallographica Section F Structural Biology and Crystallization Communications, August 2006, International Union of Crystallography

    Susan M Lea, Professor William D Picking

  32. IpaD Localizes to the Tip of the Type III Secretion System Needle of Shigella flexneri

    Article • Infection and Immunity, August 2006, ASM Journals

    Professor William D Picking

  33. Spectroscopic and Calorimetric Analyses of Invasion Plasmid Antigen D (IpaD) from Shigella flexneri Reveal the Presence of Two Structural Domains †

    Article • Biochemistry, August 2006, American Chemical Society (ACS)

    Professor William D Picking

  34. Solution Structure of Monomeric BsaL, the Type III Secretion Needle Protein of Burkholderia pseudomallei

    Article • Journal of Molecular Biology, June 2006, Elsevier

    Professor William D Picking

  35. Expression, purification, crystallization and preliminary crystallographic analysis of MxiH, a subunit of theShigella flexneritype III secretion system needle

    Article • Acta Crystallographica Section F Structural Biology and Crystallization Communications, February 2006, International Union of Crystallography

    Susan M Lea, Professor William D Picking

  36. Characterization of the Interaction of Single Tryptophan Containing Mutants of IpaC from Shigella flexneri with Phospholipid Membranes †

    Article • Biochemistry, January 2006, American Chemical Society (ACS)

    Professor William D Picking

  37. Helical Packing of Needles from Functionally Altered Shigella Type III Secretion Systems

    Article • Journal of Molecular Biology, November 2005, Elsevier

    Susan M Lea, Professor William D Picking

  38. The Needle Component of the Type III Secreton of Shigella Regulates the Activity of the Secretion Apparatus

    Article • Journal of Biological Chemistry, October 2005, American Society for Biochemistry & Molecular Biology (ASBMB)

    Professor William D Picking

  39. IpaD of Shigella flexneri Is Independently Required for Regulation of Ipa Protein Secretion and Efficient Insertion of IpaB and IpaC into Host Membranes

    Article • Infection and Immunity, March 2005, ASM Journals

    Professor William D Picking

  40. Deletion in the Shigella Enterotoxin Genes Further Attenuates Shigella flexneri 2a Bearing Guanine Auxotrophy in a Phase 1 Trial of CVD 1204 and CVD 1208

    Article • The Journal of Infectious Diseases, November 2004, Oxford University Press (OUP)

    Professor William D Picking

  41. Destabilization of phospholipid model membranes by YplA, a phospholipase A2 secreted by Yersinia enterocolitica

    Article • Chemistry and Physics of Lipids, September 2004, Elsevier

    Professor William D Picking

  42. Structural Characterization of the N Terminus of IpaC from Shigella flexneri

    Article • Infection and Immunity, March 2003, ASM Journals

    Professor William D Picking

  43. Structure-Function Analysis of Invasion Plasmid Antigen C (IpaC) from Shigella flexneri

    Article • Journal of Biological Chemistry, November 2002, American Society for Biochemistry & Molecular Biology (ASBMB)

    Professor William D Picking

  44. Purification and Characterization of Two Active Derivatives of Recombinant YplA, a Secreted Phospholipase from Yersinia entercolitica

    Article • Biochemical and Biophysical Research Communications, March 2002, Elsevier

    Professor William D Picking

  45. Cholera Toxin

    Article • January 2002, Wiley

    Professor William D Picking

  46. IpaC from Shigella and SipC from Salmonella possess similar biochemical properties but are functionally distinct

    Article • Molecular Microbiology, October 2001, Wiley

    Professor William D Picking

  47. In Vitro Assembly of Novel Cholera Toxin-like Complexes

    Article • Analytical Biochemistry, May 2001, Elsevier

    Professor William D Picking

  48. Identification of functional regions within invasion plasmid antigen C (IpaC) of Shigella flexneri

    Article • Molecular Microbiology, January 2001, Wiley

    Professor William D Picking

  49. Interaction of Shigella flexneri IpaC with Model Membranes Correlates with Effects on Cultured Cells

    Article • Infection and Immunity, June 2000, ASM Journals

    Professor William D Picking

  50. Cholera Toxin Conformational Changes Associated with Changes in Membrane Structure

    Article • May 2000, Taylor & Francis

    Professor William D Picking

  51. Shigella flexneri 2a Strain CVD 1207, with Specific Deletions in virG, sen, set, andguaBA, Is Highly Attenuated in Humans

    Article • Infection and Immunity, March 2000, ASM Journals

    Professor William D Picking

  52. Production of IFN-  and IL-10 to Shigella Invasins by Mononuclear Cells from Volunteers Orally Inoculated with a Shiga Toxin-Deleted Shigella dysenteriae Type 1 Strain

    Article • The Journal of Immunology, February 2000, The American Association of Immunologists

    Professor William D Picking

  53. Localization of the Binding Site for Modified Gb 3 on Verotoxin 1 Using Fluorescence Analysis †

    Article • Biochemistry, June 1999, American Chemical Society (ACS)

    Professor William D Picking

  54. Protein-protein interactions in the assembly of Shigella flexneri invasion plasmid antigens IpaB and IpaC into protein complexes

    Article • Biochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology, December 1998, Elsevier

    Professor William D Picking

  55. Conformational Changes in Cholera Toxin B Subunit−Ganglioside GM1 Complexes Are Elicited by Environmental pH and Evoke Changes in Membrane Structure †

    Article • Biochemistry, July 1997, American Chemical Society (ACS)

    Professor William D Picking

  56. Cloning, Expression, and Affinity Purification of RecombinantShigella flexneriInvasion Plasmid Antigens IpaB and IpaC

    Article • Protein Expression and Purification, December 1996, Elsevier

    Professor William D Picking

  57. Fluorescence Analysis of Galactose, Lactose, and Fucose Interaction with the Cholera Toxin B Subunit

    Article • Biochemical and Biophysical Research Communications, September 1996, Elsevier

    Professor William D Picking

  58. Structural Analysis of Invasion Plasmid Antigen D (Ipad) from Shigella flexneri

    Article • Biochemical and Biophysical Research Communications, September 1995, Elsevier

    Professor William D Picking

  59. Fluorescence analysis of the interaction between ganglioside GM1-containing phospholipid vesicles and the B subunit of cholera toxin

    Article • Biochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology, February 1995, Elsevier

    Professor William D Picking

  60. Evidence for RNA in the peptidyl transferase center of Escherichia coli ribosomes as indicated by fluorescence

    Article • Biochemistry, December 1992, American Chemical Society (ACS)

    Professor William D Picking

  61. Fluorescence characterization of the environment encountered by nascent polyalanine and polyserine as they exit Escherichia coli ribosomes during translation

    Article • Biochemistry, March 1992, American Chemical Society (ACS)

    Professor William D Picking

  62. Fluorescence studies on the interaction of inhibitor 2 and okadaic acid with the catalytic subunit of type 1 phosphoprotein phosphatases

    Article • Biochemistry, October 1991, American Chemical Society (ACS)

    Professor William D Picking

  63. The synthesis of polyphenylalanine on ribosomes to which erythromycin is bound

    Article • European Journal of Biochemistry, June 1991, Wiley

    Professor William D Picking

  64. Eukaryotic protein synthesis initiation factor 2. A target for inactivation by proanthocyanidin

    Article • European Journal of Biochemistry, May 1991, Wiley

    Professor William D Picking

  65. A synthetic alanyl-initiator tRNA with initiator tRNA properties as determined by flourescence measurements: comparison to a synthetic alanyl-elongator tRNA

    Article • Nucleic Acids Research, January 1991, Oxford University Press (OUP)

    Professor William D Picking

  66. Movement of tRNA but not the nascent peptide during peptide bond formation on ribosomes

    Article • Biochemistry, December 1990, American Chemical Society (ACS)

    Professor William D Picking

  67. The extension of polyphenylalanine and polylysine peptides on Escherichia coli ribosomes

    Article • Biochimica et Biophysica Acta (BBA) - Gene Structure and Expression, August 1990, Elsevier

    Professor William D Picking

  68. The Use of Fluorescence Resonance Energy Transfer to Detect Conformational Changes in Protein Toxins

    Article • Springer Science + Business Media

    Professor William D Picking