Figure 1. The binding of PEDF to PLXDC1 and PLXDC2 on cell surface.
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Upper panel: Binding of biotinylated PEDF to control HEK293 cells ( A ) or HEK293 cells transfected with PLXDC1 ( B ), PLXDC2 ( C ), intracellular domain deleted PLXDC2 ( D , PLXDC2-dC), extracellular domain (ECD) deleted PLXDC1 ( E ), or ECD deleted PLXDC2 ( F ). Binding was detected by stretavidin-alkaline phosphatase, shown as deep purple color. Lower panel: Live cell staining of an epitope tag of control HEK293 cells ( G ) or HEK293 cells transfected with PLXDC1 ( H ), PLXDC2 ( I ), intracellular domain deleted PLXDC2 ( J , PLXDC2-dC), ECD-deleted PLXDC1 ( K ), or ECD deleted PLXDC2 ( L ). All constructs have the epitope tag engineered after the secretion signal at the N-terminus.