All Stories

  1. Characterisation of Modular Polyketide Synthases Designed to Make Pentaene Analogues of Amphotericin B

    Article • Molecules, March 2024, MDPI AG

    Dr Patrick Caffrey

  2. Generation of New Glycoanalogues of Polyene Antibiotics by Synthetic Biology—Testing Current Technical Boundaries

    Article • SynBio, January 2024, MDPI AG

    Dr Patrick Caffrey

  3. Biosynthesis of a new skyllamycin in Streptomyces nodosus: a cytochrome P450 forms an epoxide in the cinnamoyl chain

    Article • Organic & Biomolecular Chemistry, January 2024, Royal Society of Chemistry

    Dr Patrick Caffrey

  4. A Biomimetic Polymer for the Extraction and Purification of Superior Analogues of Amphotericin B

    Article • Biomimetics, June 2023, MDPI AG

    Dr Patrick Caffrey

  5. Structural analysis of P450 AmphL from Streptomyces nodosus provides insights into substrate selectivity of polyene macrolide antibiotic biosynthetic P450s

    Article • Journal of Biological Chemistry, April 2022, Elsevier

    Dr Patrick Caffrey

  6. New Glycosylated Polyene Macrolides: Refining the Ore from Genome Mining

    Article • Antibiotics, March 2022, MDPI AG

    Dr Patrick Caffrey

  7. New insights into polyene macrolide biosynthesis in Couchioplanes caeruleus

    Article • Molecular BioSystems, January 2017, Royal Society of Chemistry

    Dr Patrick Caffrey

  8. Engineered biosynthesis and characterisation of disaccharide-modified 8-deoxyamphoteronolides

    Article • Applied Microbiology and Biotechnology, November 2016, Springer Science + Business Media

    Dr Patrick Caffrey

  9. Polyene macrolide biosynthesis in streptomycetes and related bacteria: recent advances from genome sequencing and experimental studies

    Article • Applied Microbiology and Biotechnology, March 2016, Springer Science + Business Media

    Dr Patrick Caffrey

  10. Corrigendum to “Dissecting complex polyketide biosynthesis” [Comput Struct Biotechnol J 3(4) (2012) 1–8]

    Article • Computational and Structural Biotechnology Journal, January 2016, Elsevier

    Dr Patrick Caffrey

  11. Exploiting the genome sequence of Streptomyces nodosus for enhanced antibiotic production

    Article • Applied Microbiology and Biotechnology, October 2015, Springer Science + Business Media

    Dr Patrick Caffrey

  12. Engineered Biosynthesis of Disaccharide-Modified Polyene Macrolides

    Article • Applied and Environmental Microbiology, August 2013, ASM Journals

    Dr Patrick Caffrey

  13. Versatility of Enzymes Catalyzing Late Steps in Polyene 67-121C Biosynthesis

    Article • Bioscience Biotechnology and Biochemistry, April 2013, Taylor & Francis

    Dr Patrick Caffrey

  14. DISSECTING COMPLEX POLYKETIDE BIOSYNTHESIS

    Article • Computational and Structural Biotechnology Journal, October 2012, Elsevier

    Dr Patrick Caffrey

  15. Streptomyces nodosusHost Strains Optimized for Polyene Glycosylation Engineering

    Article • Bioscience Biotechnology and Biochemistry, February 2012, Taylor & Francis

    Dr Patrick Caffrey

  16. A labile point in mutant amphotericin polyketide synthases

    Article • Biotechnology Letters, January 2011, Springer Science + Business Media

    Dr Patrick Caffrey

  17. Redesign of Polyene Macrolide Glycosylation: Engineered Biosynthesis of 19-(O)-Perosaminyl- Amphoteronolide B

    Article • Chemistry & Biology, February 2010, Elsevier

    Dr Patrick Caffrey

  18. Isolation and characterisation of amphotericin B analogues and truncated polyketide intermediates produced by genetic engineering of Streptomyces nodosus

    Article • Organic & Biomolecular Chemistry, January 2010, Royal Society of Chemistry

    Dr Patrick Caffrey

  19. Phosphomannose isomerase and phosphomannomutase gene disruptions in Streptomyces nodosus: Impact on amphotericin biosynthesis and implications for glycosylation engineering

    Article • Metabolic Engineering, January 2009, Elsevier

    Dr Patrick Caffrey

  20. Chapter 11 Genetic Analysis of Nystatin and Amphotericin Biosynthesis

    Article • January 2009, Elsevier

    Dr Patrick Caffrey

  21. Effects of new amphotericin analogues on the scrapie isoform of the prion protein

    Article • Biochimica et Biophysica Acta (BBA) - General Subjects, October 2008, Elsevier

    Dr Patrick Caffrey, Dr Hilary E M McMahon

  22. Biosynthetic Engineering of Polyene Macrolides Towards Generation of Improved Antifungal and Antiparasitic Agents

    Article • Current Topics in Medicinal Chemistry, May 2008, Bentham Science Publishers

    Dr Patrick Caffrey

  23. Engineered Synthesis of 7-Oxo- and 15-Deoxy-15-Oxo-Amphotericins: Insights into Structure-Activity Relationships in Polyene Antibiotics

    Article • Chemistry & Biology, January 2008, Elsevier

    Dr Patrick Caffrey

  24. The Stereochemistry of Ketoreduction

    Article • Chemistry & Biology, October 2005, Elsevier

    Dr Patrick Caffrey

  25. Biosynthesis of Amphotericin Derivatives Lacking Exocyclic Carboxyl Groups

    Article • Journal of Biological Chemistry, August 2005, American Society for Biochemistry & Molecular Biology (ASBMB)

    Dr Patrick Caffrey

  26. Analysis and manipulation of amphotericin biosynthetic genes by means of modified phage KC515 transduction techniques

    Article • Gene, December 2004, Elsevier

    Dr Patrick Caffrey

  27. New Start and Finish for Complex Polyketide Biosynthesis

    Article • Chemistry & Biology, February 2004, Elsevier

    Dr Patrick Caffrey

  28. New Start and Finish for Complex Polyketide Biosynthesis

    Article • Chemistry & Biology, February 2004, Elsevier

    Dr Patrick Caffrey

  29. Biosynthesis of Deoxyamphotericins and Deoxyamphoteronolides by Engineered Strains of Streptomyces nodosus

    Article • Chemistry & Biology, December 2003, Elsevier

    Dr Patrick Caffrey

  30. Conserved Amino Acid Residues Correlating With Ketoreductase Stereospecificity in Modular Polyketide Synthases

    Article • ChemBioChem, June 2003, Wiley

    Dr Patrick Caffrey

  31. Amphotericin Biosynthesis in Streptomyces nodosus

    Article • Chemistry & Biology, January 2003, Elsevier

    Dr Patrick Caffrey

  32. Amphotericin biosynthesis in Streptomyces nodosus: deductions from analysis of polyketide synthase and late genes

    Article • Chemistry & Biology, July 2001, Elsevier

    Dr Patrick Caffrey

  33. Evidence for a double-helical structure for modular polyketide synthases

    Article • Nature Structural Biology, February 1996, Springer Science + Business Media

    Dr Patrick Caffrey

  34. The thioesterase of the erythromycin-producing polyketide synthase: mechanistic studies in vitro to investigate its mode of action and substrate specificity

    Article • Journal of the Chemical Society Chemical Communications, January 1995, Royal Society of Chemistry

    Dr Patrick Caffrey

  35. Identification of DEBS 1, DEBS 2 and DEBS 3, the multienzyme polypeptides of the erythromycin-producing polyketide synthase fromSaccharopolyspora erythraea

    Article • FEBS Letters, June 1992, Wiley

    Dr Patrick Caffrey

  36. An acyl-carrier-protein - thioesterase domain from the 6-deoxyerythronolide B synthase of Saccharopolyspora erythraea. High-level production, purification and characterisation in Escherichia coli

    Article • European Journal of Biochemistry, February 1991, Wiley

    Dr Patrick Caffrey

  37. Outer Membrane Proteins: Old and New

    Article • January 1991, Springer Science + Business Media

    Dr Patrick Caffrey