Current affiliation: Rush UniversitySubject: Life SciencesPrimary location: United States
Published in:Journal of The Royal Society InterfacePublication date:2013-12-18
This review discusses selected unique properties of HV1 from a teleological perspective. Why does it need to be so selective? Why bother to dimer...
Published in:NaturePublication date:2006-01-01
Published in:NaturePublication date:2006-02-15
Published in:NaturePublication date:2012-09-26
Published in:NaturePublication date:2013-05-01
Published in:The Journal of PhysiologyPublication date:2001-09-01
Human eosinophils are shown to have thrice larger electron currents than neutrophils, consistent with their higher rate of superoxide production. ...
Published in:NaturePublication date:2003-04-03
The voltage dependence of NADPH oxidase activity (electron current) is determined. Enzyme activity is abolished by extreme depolarization of the c...
Published in:The Journal of PhysiologyPublication date:2002-11-01
More than 700 papers had been published on ion channels in lymphocytes before we finally showed that they have proton channels too!
Published in:The Journal of PhysiologyPublication date:2008-11-15
Voltage-gated proton channels
I list the major questions that remain; there seem to be more questions than answers. This means proton channels will continue to entertain us fo...
Published in:Current Pharmaceutical DesignPublication date:2007-08-01
We review the pharmacology of proton channels; in a word, Zinc!
Published in:Progress in NeurobiologyPublication date:2001-06-01
We review the properties of proton channels in all cells, but focus on special roles in microglia.
Published in:Neutrophil Methods and ProtocolsPublication date:2014-01-01
Published in:Neutrophil Methods and ProtocolsPublication date:2007-01-01
Published in:The Journal of PhysiologyPublication date:2003-07-01
The temperature dependence of NADPH oxidase activity is determined. The intrinsic Ea of electron flux is low (only 14 kcal/mol), but that of the s...
Published in:Cellular Physiology and BiochemistryPublication date:1997-01-01
Published in:Israel Journal of ChemistryPublication date:1999-01-01
Published in:Biophysical JournalPublication date:2013-01-01
Published in:ChannelsPublication date:2010-08-15
In an Addendum to the Zn2+ paper below, we propose that the main function of the C terminus is to tether the two protomers together, because the ta...
Published in:Biochimica et Biophysica Acta (BBA) - BioenergeticsPublication date:2000-05-01
Published in:Trends in Cell BiologyPublication date:2011-01-01
Published in:NaturePublication date:2011-10-23
By swapping key residues from C15orf27, a molecule that is NOT a proton channel, into the human proton channel hHv1, we identify Asp112 in the midd...
Published in:FEBS LettersPublication date:2003-10-08
The physiological interaction between NADPH oxidase and voltage-gated proton channels in phagocytes is reviewed. A putative electron pathway throu...
Published in:Biophysical JournalPublication date:2010-01-01
Published in:The Journal of PhysiologyPublication date:2005-11-24
pH dependence of NADPH oxidase
NADPH oxidase is strongly inhibited above or below pHi 7.5, but is not affected by pHo. Surprisingly, electron current in inside-out patches (refl...
Arachidonic acid enhances proton channel gating similarly to PMA. The data are mostly consistent with the proposal by Henderson et al (1993) that ...
Published in:FEBS LettersPublication date:2008-12-10
The title says it all! We describe the inexplicable interactions between proton channels and NADPH oxidase, and discuss several kinds of mechanism...
Published in:NaturePublication date:1984-02-02
This was published in the early days of patch-clamping, when it was novel to record from a new cell type. This study illustrated the power of whol...
Published in:Wiley Interdisciplinary Reviews Membrane Transport and SignallingPublication date:2012-05-11
Published in:Biochimica et Biophysica Acta (BBA) - BioenergeticsPublication date:2006-08-01
After saying for years "charge compensation by proton channels is necessary to keep the electrogenic NADPH oxidase working," we realize that in add...
Published in:Proceedings of the National Academy of SciencesPublication date:2000-05-23
We show that human neutrophils studied using perforated-patch conformation respond dramatically to stimulation with PMA. Electron currents turn on...
Published in:The Journal of PhysiologyPublication date:2007-03-01
PKC not cPLA2α regulates proton channels
We test and discard the prevailing hypothesis that proton channels are activated by arachidonic acid (AA) produced by cPLA2a. Specific blockers hav...
Published in:The Journal of PhysiologyPublication date:2010-04-30
Cooperative gating of proton channels
We compare the properties of monomeric vs. dimeric proton channels. Zn2+ was thought to inhibit proton currents by binding competitively with prot...
Published in:Proceedings of the National Academy of SciencesPublication date:2008-07-29
Proton channels in human basophils exhibit enhanced gating when stimulated with secretagogues, PMA or anti-IgE. Inhibition of histamine release by...
Published in:Nature ImmunologyPublication date:2010-02-07
A cast of thousands was needed to describe the multiple functions of proton channels in human B lymphocytes!
Published in:SciencePublication date:2009-01-09
Published in:Biophysical JournalPublication date:1991-11-01
This is the first identification of proton currents in mammalian cells. Previously they were seen in snail neurons (Thomas & Meech, 1982) and newt...
Published in:Comprehensive PhysiologyPublication date:2012-04-01
Published in:NaturePublication date:2015-04-29
Published in:NaturePublication date:2011-05-05
Published in:Biophysical JournalPublication date:2009-02-01
Published in:Scientific ReportsPublication date:2015-05-08
Mutations and MD (molecular dynamics) identified the requirements for proton selectivity of HV1, but could not answer the 2-decade-old question: D...
Published in:Mechanisms of Lymphocyte Activation and Immune RegulationPublication date:1987-01-01
Published in:Progress in Molecular Biology and Translational SciencePublication date:2013-01-01
We describe the properties of dimeric and monomeric forms of HV1 and discuss the mystery of why the mammalian channel exists as a dimer
Published in:Biophysical JournalPublication date:2012-01-01
Published in:BiochemistryPublication date:2015-06-02
This review updates unique properties of HV1 with a target audience of non-electrophysiologists.
Published in:Biophysical JournalPublication date:2015-01-01
Published in:The Journal of PhysiologyPublication date:1995-12-01
This is the second excised-patch study of proton currents. We find that pHi strongly affects channel opening kinetics.
Published in:Physiological ReviewsPublication date:2003-04-01
Voltage-gated proton channels are reviewed, along with other proton-conducting molecules for comparison. Valuable prizes will be awarded to those w...
Published in:Proceedings of the National Academy of SciencesPublication date:2014-11-25
We find that human B lymphocytes express two isoforms of the voltage-gated proton channel, HVCN1 - the full-length 273 amino acid Long form, and a ...
Published in:Biophysical JournalPublication date:1996-07-01
Buffer deprotonation is not the rate-limiting step on proton permeation. We show that external buffer concentration has little effect between 1-10...
Published in:The Journal of PhysiologyPublication date:1988-01-01
Published in:The Journal of PhysiologyPublication date:1984-04-01
Published in:Biophysical JournalPublication date:1993-10-01
We confirm the prediction of Henderson, Chappell & Jones (1987) that human neutrophil do have voltage-gated proton channels. These channels respon...
Published in:Experimental NeurologyPublication date:1978-09-01
Published in:Muscle & NervePublication date:1982-10-01
Published in:AJP Cell PhysiologyPublication date:2011-10-19
Human monocytes have proton channels and NADPH oxidase like other phagocytes. PMA stimulation produces enhanced gating of HV1 and electron current...
Published in:Physiological ReviewsPublication date:2013-04-01
Ten Years After! Let's see - now the proton channel has genes! The protein was a huge surprise - a self-standing VSD with strong similarities to,...
Published in:Science SignalingPublication date:2004-05-11
This Perspective critiques a recent paper by the Segal group (Ahluwalia et al, 2004. Nature 427:853-858) which reports that maxi-K channels are the...
Published in:Journal of Clinical ImmunologyPublication date:1985-01-01
Published in:Cellular and Molecular Life SciencesPublication date:2008-05-09
Published in:Cellular and Molecular Life SciencesPublication date:2005-08-29
Published in:The Journal of Membrane BiologyPublication date:1994-09-01
Published in:The Journal of General PhysiologyPublication date:1987-03-01
We show that there are two distinct types of K+ channels in mouse lymphocytes. These were later identified as Kv1.3 and Kv3.1.
Published in:PhysiologyPublication date:2010-02-01
The latest proton channel lore is updated, with a focus on the role in the phagocyte respiratory burst. New cartoons! There is a remarkable symbi...
Published in:The Journal of General PhysiologyPublication date:2002-11-25
We summarize the arguments against the hypothesis that one component of NADPH oxidase functions as a proton channel.
Published in:Journal of NeuroimmunologyPublication date:1985-11-01
Published in:The Journal of Membrane BiologyPublication date:1996-07-01
Published in:The Journal of General PhysiologyPublication date:1995-10-01
Published in:The Journal of PhysiologyPublication date:1985-01-01
Published in:The Journal of General PhysiologyPublication date:1994-05-01
We show that despite perfusing cells with 100 mM buffer, pHi changes when Na+/H+ antiport is active.
Published in:The Journal of General PhysiologyPublication date:1990-04-01
I show evidence that suggests that inactivation of Kv1.3 channels (in rat alveolar epithelial cells) occurs exclusively from the open state. In ot...
Published in:Journal of Allergy and Clinical ImmunologyPublication date:1985-01-01
Published in:SciencePublication date:1986-09-12
Published in:The Journal of General PhysiologyPublication date:1998-10-01
We show that both the conductance and gating kinetics of proton channels are more strongly temperature dependent than almost any other ion channel ...
Published in:The Journal of General PhysiologyPublication date:1997-04-01
Voltage-gated proton currents are studied in heavy water. Deuterium can permeate, but does so only half as well as protons, suggesting a more comp...
Published in:Proceedings of the National Academy of SciencesPublication date:2011-10-17
In 1972, Woody Hastings became the first human being to propose the existence of voltage-gated proton channels. He postulated that they trigger th...
Published in:The Journal of General PhysiologyPublication date:2013-11-11
We move the selectivity filter Asp112 of hHV1 up and down the S1 transmembrane segment. Three positions allow current, 109, 112 (WT) and 116; all ...
Published in:Proceedings of the National Academy of SciencesPublication date:2009-10-05
We examine cytoplasmic pH of human neutrophils during phagocytosis using confocal imaging, SNARF-1, and SEER. Convention says Na/H antiport is the...
Published in:AJP Cell PhysiologyPublication date:2007-03-14
This provides the context for the study: Essin, K., B. Salanova, R. Kettritz, M. Sausbier, F.C. Luft, D. Kraus, E. Bohn, I.B. Autenrieth, A. Pesch...
Published in:The Journal of General PhysiologyPublication date:2013-03-25
We evaluate two open-state models of hHV1, using extensive molecular dynamics simulations and experiment. Both approaches lead to a model with an ...
Published in:Journal of Biological ChemistryPublication date:2009-12-26
We identify Thr29 as the main phosphorylation site on the proton channel. Phosphorylating this site on the intracellular N terminus appears to con...
Published in:Journal of Biological ChemistryPublication date:2012-01-30
NOX5 is the main form of NADPH oxidase in human sperm, proton channels help regulate ROS production, and ROS production contributes to sperm motility.
Published in:The Journal of PhysiologyPublication date:2008-05-15
Voltage-dependent proton channel gating
We express the recently-identified human and mouse proton channel genes in HEK-293 or COS-7 cells. Although in nearly every respect, the currents ...
Published in:Journal of Biological ChemistryPublication date:2001-07-26
Identical proton currents are found in control PLB-985 cells, gp91phox knock-outs (and re-transfectants) and in human CGD phagocytes that lack gp91...
Published in:The Journal of General PhysiologyPublication date:2015-10-12
Part of the “signature sequence” that defines voltage gated proton channels (HV1) is a tryptophan residue adjacent to the second Arg in the S4 tran...
Published in:Biophysical JournalPublication date:2014-01-01
Published in:Biophysical JournalPublication date:2011-02-01
Published in:The Journal of General PhysiologyPublication date:1998-06-01
Published in:Proceedings of the National Academy of SciencesPublication date:2015-10-14
I discuss an EPR study of the human proton channel by Li et al (2015) PNAS. They propose that hHV1 opens by the S4 helix moving up "one-click." Mo...
Published in:Biophysical JournalPublication date:2016-02-01
Published in:The Journal of General PhysiologyPublication date:2003-05-27
We determine the conductance of a single proton channel. We claim the world's smallest directly-measured single-channel currents (7-16 fA). Noise...
Published in:The Journal of General PhysiologyPublication date:1999-11-29
We show that low pH strongly inhibits the ability of Zn2+ to inhibit proton currents.
Published in:The Journal of General PhysiologyPublication date:2002-05-28
This paper counters arguments raised against the paper listed next. We show that cells that lack endogenous proton channels still lack them when t...
Published in:The Journal of General PhysiologyPublication date:2006-05-15
Published in:Immunological ReviewsPublication date:2016-08-25
This review gives a historical account of the elucidation of the relationship between voltage-gated proton channels and the NADPH oxidase (NOX2) of...
Published in:The Journal of General PhysiologyPublication date:2016-08-01
Published in:The Journal of General PhysiologyPublication date:2004-06-28
Published in:AJP Lung Cellular and Molecular PhysiologyPublication date:2004-10-15
Published in:Physiological ReviewsPublication date:2004-10-01
Published in:The Journal of General PhysiologyPublication date:1995-06-01
Published in:The Journal of PhysiologyPublication date:2017-10-11