All Stories

  1. Fungal-bacterial gut microbiota interactions in patients withClostridioides difficilecolonisation and infection

    Article • July 2023, Cold Spring Harbor Laboratory Press

    Dr Wiep Klaas Smits

  2. The vanR Cd Mutation 343A>G, Resulting in a Thr115Ala Substitution, Is Associated with an Elevated Minimum Inhibitory Concentration (MIC) of Vancomycin in Clostridio...

    Article • Microbiology Spectrum, June 2023, ASM Journals

    Dr Wiep Klaas Smits

  3. Clostridioides difficile infection with isolates of cryptic clade C-II: a genomic analysis of polymerase chain reaction ribotype 151

    Article • Clinical Microbiology and Infection, April 2023, Elsevier

    Dr Wiep Klaas Smits

  4. Non-Toxigenic Clostridioides difficile Strain E4 (NTCD-E4) Prevents Establishment of Primary C. difficile Infection by Epidemic PCR Ribotype 027 in an In Vitro Human Gut Model

    Article • Antibiotics, February 2023, MDPI AG

    Dr Wiep Klaas Smits

  5. Carriage of three plasmids in a single human clinical isolate of Clostridioides difficile

    Article • Plasmid, January 2023, Elsevier

    Dr Wiep Klaas Smits

  6. In-Depth Characterization of the Clostridioides difficile Phosphoproteome to Identify Ser/Thr Kinase Substrates

    Article • Molecular & Cellular Proteomics, November 2022, Elsevier

    Dr Wiep Klaas Smits

  7. Sequence-Based Identification of Metronidazole-Resistant Clostridioides difficile Isolates

    Article • Emerging Infectious Diseases, November 2022, Centers for Disease Control and Prevention (CDC)

    Dr Wiep Klaas Smits

  8. 2’-Fucosyllactose inhibits proliferation of Clostridioides difficile ATCC 43599 in the CDi-screen, an in vitro model simulating Clostridioides difficile infection

    Article • Frontiers in Cellular and Infection Microbiology, October 2022, Frontiers

    Dr Wiep Klaas Smits

  9. International travel, the gut microbiome, and ESBL-E coli carriage – Authors' reply

    Article • The Lancet Microbe, October 2022, Elsevier

    Dr Wiep Klaas Smits

  10. Clostridioides difficilePCR ribotype 151 is polyphyletic and includes pathogenic isolates from cryptic clade C-II with mono-toxin pathogenicity loci that can escape routine diagnostics

    Article • August 2022, Cold Spring Harbor Laboratory Press

    Dr Wiep Klaas Smits

  11. Carriage of three plasmids in a single human clinical isolate of Clostridioides difficile

    Article • July 2022, Cold Spring Harbor Laboratory Press

    Dr Wiep Klaas Smits

  12. New insights into the type A glycan modification of Clostridioides difficile flagellar protein flagellin C by phosphoproteomics analysis

    Article • Journal of Biological Chemistry, March 2022, Elsevier

    Dr Wiep Klaas Smits

  13. Clostridioides difficile Phosphoproteomics Shows an Expansion of Phosphorylated Proteins in Stationary Growth Phase

    Article • mSphere, February 2022, ASM Journals

    Dr Wiep Klaas Smits

  14. Comparison of Whole-Genome Sequence-Based Methods and PCR Ribotyping for Subtyping of Clostridioides difficile

    Article • Journal of Clinical Microbiology, February 2022, ASM Journals

    Dr Wiep Klaas Smits

  15. Plasmids of Clostridioides difficile

    Article • Current Opinion in Microbiology, February 2022, Elsevier

    Dr Wiep Klaas Smits

  16. Practical observations on the use of fluorescent reporter systems in Clostridioides difficile

    Article • Antonie van Leeuwenhoek, January 2022, Springer Science + Business Media

    Dr Wiep Klaas Smits

  17. Host Immune Responses to Clostridioides difficile: Toxins and Beyond

    Article • Frontiers in Microbiology, December 2021, Frontiers

    Dr Wiep Klaas Smits

  18. Clostridioides difficile phosphoproteomics shows an expansion of phosphorylated proteins in stationary growth phase

    Article • November 2021, Cold Spring Harbor Laboratory Press

    Dr Wiep Klaas Smits

  19. Fecal Microbiota Transplantation Influences Procarcinogenic Escherichia coli in Recipient Recurrent Clostridioides difficile Patients

    Article • Gastroenterology, October 2021, Elsevier

    Dr Wiep Klaas Smits

  20. Performance of Core Genome Multilocus Sequence Typing Compared to Capillary-Electrophoresis PCR Ribotyping and SNP Analysis ofClostridioides difficile

    Article • August 2021, Cold Spring Harbor Laboratory Press

    Dr Wiep Klaas Smits

  21. Practical observations on the use of fluorescent reporter systems in C. difficile

    Article • June 2021, Cold Spring Harbor Laboratory Press

    Dr Wiep Klaas Smits

  22. Haem is crucial for medium-dependent metronidazole resistance in clinical isolates of Clostridioides difficile

    Article • Journal of Antimicrobial Chemotherapy, April 2021, Oxford University Press (OUP)

    Dr Wiep Klaas Smits

  23. Cyclodextrin/Adamantane-Mediated Targeting of Inoculated Bacteria in Mice

    Article • Bioconjugate Chemistry, February 2021, American Chemical Society (ACS)

    Dr Wiep Klaas Smits

  24. Heme is crucial for medium-dependent metronidazole resistance in clinical isolates of C. difficile

    Article • November 2020, Cold Spring Harbor Laboratory Press

    Dr Wiep Klaas Smits

  25. Distinct evolution of colistin resistance associated with experimental resistance evolution models in Klebsiella pneumoniae

    Article • Journal of Antimicrobial Chemotherapy, November 2020, Oxford University Press (OUP)

    Dr Wiep Klaas Smits

  26. Redefining the Clostridioides difficile σ B Regulon: σ B Activates Genes Involved in Detoxifying Radicals That Can Result from the Exposure to Antimicrobials and Hydrogen Peroxide

    Article • mSphere, October 2020, ASM Journals

    Dr Wiep Klaas Smits

  27. Identification of the Unwinding Region in the Clostridioides difficile Chromosomal Origin of Replication

    Article • Frontiers in Microbiology, October 2020, Frontiers

    Dr Wiep Klaas Smits

  28. The C-Terminal Domain of Clostridioides difficile TcdC Is Exposed on the Bacterial Cell Surface

    Article • Journal of Bacteriology, August 2020, ASM Journals

    Dr Wiep Klaas Smits

  29. Redefining the Clostridioides difficile σB regulon: σB activates genes involved in detoxifying radicals that can result from the exposure to antimicrobials and hydrogen peroxide

    Article • July 2020, Cold Spring Harbor Laboratory Press

    Dr Wiep Klaas Smits

  30. Identification of the unwinding region in theClostridioides difficilechromosomal origin of replication

    Article • July 2020, Cold Spring Harbor Laboratory Press

    Dr Wiep Klaas Smits

  31. Plasmid-mediated metronidazole resistance in Clostridioides difficile

    Article • Nature Communications, January 2020, Springer Science + Business Media

    Dr Wiep Klaas Smits

  32. The C-terminal domain of Clostridioides difficile TcdC is exposed on the bacterial cell surface

    Article • December 2019, Cold Spring Harbor Laboratory Press

    Dr Wiep Klaas Smits

  33. An in silico survey of Clostridioides difficile extrachromosomal elements

    Article • Microbial Genomics, September 2019, Society for General Microbiology

    Dr Wiep Klaas Smits

  34. Microbial evolutionary medicine: from theory to clinical practice

    Article • The Lancet Infectious Diseases, August 2019, Elsevier

    Dr Wiep Klaas Smits

  35. #EUROmicroMOOC: using Twitter to share trends in Microbiology worldwide

    Article • FEMS Microbiology Letters, June 2019, Oxford University Press (OUP)

    Dr Thibault G Sana, Manuel Sanchez, Dr Wiep Klaas Smits

  36. Anin silicosurvey ofClostridioides difficileextrachromosomal elements

    Article • May 2019, Cold Spring Harbor Laboratory Press

    Dr Wiep Klaas Smits

  37. Plasmid-mediated metronidazole resistance in Clostridioides difficile

    Article • May 2019, Cold Spring Harbor Laboratory Press

    Dr Wiep Klaas Smits

  38. Multimodal Tracking of Controlled Staphylococcus aureus Infections in Mice

    Article • ACS Infectious Diseases, April 2019, American Chemical Society (ACS)

    Dr. Mick Welling, Dr Wiep Klaas Smits

  39. Fluorescent imaging of bacterial infections and recent advances made with multimodal radiopharmaceuticals

    Article • Clinical and Translational Imaging, April 2019, Springer Science + Business Media

    Dr. Mick Welling, Dr Wiep Klaas Smits

  40. The Bacterial Chromatin Protein HupA Can Remodel DNA and Associates with the Nucleoid in Clostridium difficile

    Article • Journal of Molecular Biology, February 2019, Elsevier

    Dr Wiep Klaas Smits

  41. Characterization of the virulence of a non-RT027, non-RT078 and binary toxin-positive Clostridium difficile strain associated with severe diarrhea

    Article • Emerging Microbes & Infections, December 2018, Taylor & Francis

    Dr Wiep Klaas Smits

  42. Genome Location Dictates the Transcriptional Response to PolC Inhibition in Clostridium difficile

    Article • Antimicrobial Agents and Chemotherapy, November 2018, ASM Journals

    Dr Wiep Klaas Smits

  43. The evolving epidemic of Clostridium difficile 630

    Article • Anaerobe, October 2018, Elsevier

    Dr Wiep Klaas Smits

  44. The bacterial chromatin protein HupA can remodel DNA and associates with the nucleoid in Clostridium difficile

    Article • September 2018, Cold Spring Harbor Laboratory Press

    Dr Wiep Klaas Smits

  45. Genome location dictates the transcriptional response to PolC-inhibition inClostridium difficile

    Article • July 2018, Cold Spring Harbor Laboratory Press

    Dr Wiep Klaas Smits

  46. Mechanistic Insights in the Success of Fecal Microbiota Transplants for the Treatment of Clostridium difficile Infections

    Article • Frontiers in Microbiology, June 2018, Frontiers

    Dr Wiep Klaas Smits

  47. Proteomic identification of Axc, a novel beta-lactamase with carbapenemase activity in a meropenem-resistant clinical isolate of Achromobacter xylosoxidans

    Article • Scientific Reports, May 2018, Springer Science + Business Media

    Dr Wiep Klaas Smits

  48. A helicase-containing module defines a family of pCD630-like plasmids in Clostridium difficile

    Article • Anaerobe, February 2018, Elsevier

    Dr Wiep Klaas Smits

  49. A helicase-containing module defines a family of pCD630-like plasmids in Clostridium difficile

    Article • October 2017, Cold Spring Harbor Laboratory Press

    Dr Wiep Klaas Smits

  50. Proteomic identification of Axc, a novel beta-lactamase with carbapenemase activity in a meropenem-resistant clinical isolate of Achromobacter xylosoxidans

    Article • September 2017, Cold Spring Harbor Laboratory Press

    Dr Wiep Klaas Smits

  51. DNA replication proteins as potential targets for antimicrobials in drug-resistant bacterial pathogens

    Article • Journal of Antimicrobial Chemotherapy, January 2017, Oxford University Press (OUP)

    Dr Wiep Klaas Smits

  52. Primase is required for helicase activity and helicase alters the specificity of primase in the enteropathogenClostridium difficile

    Article • Open Biology, December 2016, Royal Society Publishing

    Dr Wiep Klaas Smits

  53. Interspecies Interactions between Clostridium difficile and Candida albicans

    Article • mSphere, November 2016, ASM Journals

    Dr Wiep Klaas Smits

  54. SNP-ing out the differences: Investigating differences between Clostridium difficile lab strains

    Article • Virulence, October 2016, Taylor & Francis

    Dr Wiep Klaas Smits

  55. Interactions between helicase and primase are crucial for DNA replication in the enteropathogen Clostridium difficile

    Article • August 2016, Cold Spring Harbor Laboratory Press

    Dr Wiep Klaas Smits

  56. The Signal Sequence of the Abundant Extracellular Metalloprotease PPEP-1 Can Be Used to Secrete Synthetic Reporter Proteins in Clostridium difficile

    Article • ACS Synthetic Biology, June 2016, American Chemical Society (ACS)

    Dr Wiep Klaas Smits

  57. Clostridium difficile infection

    Article • Nature Reviews Disease Primers, April 2016, Nature

    Dr Wiep Klaas Smits

  58. Clostridium difficile infection

    Article • Nature Reviews Disease Primers, April 2016, Springer Science + Business Media

    Dr Wiep Klaas Smits

  59. Complete genome sequence of BS49 and draft genome sequence of BS34A, Bacillus subtilis strains carrying Tn916

    Article • FEMS Microbiology Letters, February 2015, Oxford University Press (OUP)

    Dr Wiep Klaas Smits

  60. Complete genome sequence of the Clostridium difficile laboratory strain 630Δerm reveals differences from strain 630, including translocation of the mobile element CTn5

    Article • BMC Genomics, January 2015, Springer Science + Business Media

    Dr Wiep Klaas Smits

  61. The HtrA-Like Protease CD3284 Modulates Virulence of Clostridium difficile

    Article • Infection and Immunity, July 2014, ASM Journals

    Dr Wiep Klaas Smits, Dr Jeroen Corver

  62. Functional genomics reveals that Clostridium difficileSpo0A coordinates sporulation, virulence and metabolism

    Article • BMC Genomics, February 2014, Springer Science + Business Media

    Dr Wiep Klaas Smits

  63. Hype or hypervirulence

    Article • Virulence, October 2013, Taylor & Francis

    Dr Wiep Klaas Smits

  64. C. difficile 630Δerm Spo0A Regulates Sporulation, but Does Not Contribute to Toxin Production, by Direct High-Affinity Binding to Target DNA

    Article • PLoS ONE, October 2012, PLOS

    Dr Wiep Klaas Smits

  65. TcdC Does Not Significantly Repress Toxin Expression in Clostridium difficile 630ΔErm

    Article • PLoS ONE, August 2012, PLOS

    Dr Wiep Klaas Smits, Dr Jeroen Corver

  66. Chromosomal Replication Initiation Machinery of Low-G+C-Content Firmicutes

    Article • Journal of Bacteriology, July 2012, ASM Journals

    Dr Wiep Klaas Smits

  67. Untwisting of the DNA helix stimulates the endonuclease activity of Bacillus subtilis Nth at AP sites

    Article • Nucleic Acids Research, September 2011, Oxford University Press (OUP)

    Dr Wiep Klaas Smits

  68. Primosomal Proteins DnaD and DnaB Are Recruited to Chromosomal Regions Bound by DnaA in Bacillus subtilis

    Article • Journal of Bacteriology, November 2010, ASM Journals

    Dr Wiep Klaas Smits

  69. The Transcriptional Regulator Rok Binds A+T-Rich DNA and Is Involved in Repression of a Mobile Genetic Element in Bacillus subtilis

    Article • PLoS Genetics, November 2010, PLOS

    Dr Wiep Klaas Smits

  70. When simple sequence comparison fails: the cryptic case of the shared domains of the bacterial replication initiation proteins DnaB and DnaD

    Article • Nucleic Acids Research, June 2010, Oxford University Press (OUP)

    Dr Wiep Klaas Smits

  71. Ordered association of helicase loader proteins with the Bacillus subtilis origin of replication in vivo

    Article • Molecular Microbiology, January 2010, Wiley

    Dr Wiep Klaas Smits

  72. Ubiquitous late competence genes in Bacillus species indicate the presence of functional DNA uptake machineries

    Article • Environmental Microbiology, August 2009, Wiley

    Dr Wiep Klaas Smits, Dr Akos T Kovacs

  73. Bistability, Epigenetics, and Bet-Hedging in Bacteria

    Article • Annual Review of Microbiology, October 2008, Annual Reviews

    Dr Wiep Klaas Smits

  74. Phenotypic Variation and Bistable Switching in Bacteria

    Article • January 2008, Springer Science + Business Media

    Dr Wiep Klaas Smits

  75. Production and Secretion Stress Caused by Overexpression of Heterologous α-Amylase Leads to Inhibition of Sporulation and a Prolonged Motile Phase in Bacillus subtilis

    Article • Applied and Environmental Microbiology, June 2007, ASM Journals

    Dr Wiep Klaas Smits

  76. Temporal separation of distinct differentiation pathways by a dual specificity Rap‐Phr system in Bacillus subtilis

    Article • Molecular Microbiology, June 2007, Wiley

    Dr Wiep Klaas Smits

  77. A Single, Specific Thymine Mutation in the ComK-Binding Site Severely Decreases Binding and Transcription Activation by the Competence Transcription Factor ComK of Bacillus subtilis

    Article • Journal of Bacteriology, April 2007, ASM Journals

    Dr Wiep Klaas Smits

  78. Antirepression as a second mechanism of transcriptional activation by a minor groove binding protein

    Article • Molecular Microbiology, April 2007, Wiley

    Dr Wiep Klaas Smits, Professor David A Dubnau

  79. Single cell analysis of gene expression patterns of competence development and initiation of sporulation in Bacillus subtilis grown on chemically defined media

    Article • Journal of Applied Microbiology, September 2006, Oxford University Press (OUP)

    Dr Wiep Klaas Smits

  80. Phenotypic variation in bacteria: the role of feedback regulation

    Article • Nature Reviews Microbiology, April 2006, Springer Science + Business Media

    Dr Wiep Klaas Smits

  81. Tricksy Business: Transcriptome Analysis Reveals the Involvement of Thioredoxin A in Redox Homeostasis, Oxidative Stress, Sulfur Metabolism, and Cellular Differentiation in Bacillus subtilis

    Article • Journal of Bacteriology, June 2005, ASM Journals

    Dr Wiep Klaas Smits

  82. Stripping Bacillus: ComK auto‐stimulation is responsible for the bistable response in competence development

    Article • Molecular Microbiology, March 2005, Wiley

    Dr Wiep Klaas Smits

  83. The Rok Protein of Bacillus subtilis Represses Genes for Cell Surface and Extracellular Functions

    Article • Journal of Bacteriology, March 2005, ASM Journals

    Dr Wiep Klaas Smits

  84. Visualization of Differential Gene Expression by Improved Cyan Fluorescent Protein and Yellow Fluorescent Protein Production in Bacillus subtilis

    Article • Applied and Environmental Microbiology, November 2004, ASM Journals

    Dr Wiep Klaas Smits

  85. Article • Genome Biology, January 2004, Springer Science + Business Media

    Dr Wiep Klaas Smits

  86. Improving the predictive value of the competence transcription factor (ComK) binding site in Bacillus subtilis using a genomic approach

    Article • Nucleic Acids Research, December 2002, Oxford University Press (OUP)

    Dr Wiep Klaas Smits