All Stories

  1. Structural characterization of a nonhydrolyzing UDP-GlcNAc 2-epimerase fromNeisseria meningitidisserogroup A

    Article • Acta Crystallographica Section F Structural Biology Communications, October 2020, International Union of Crystallography

    Professor Xi Chen

  2. A Photobacterium sp. α2–6-sialyltransferase (Psp2,6ST) mutant with an increased expression level and improved activities in sialylating Tn antigens

    Article • Carbohydrate Research, May 2015, Elsevier

    Professor Xi Chen

  3. Structures ofBacteroides fragilisuridine 5′-diphosphate-N-acetylglucosamine (UDP-GlcNAc) acyltransferase (BfLpxA)

    Article • Acta Crystallographica Section D Biological Crystallography, April 2015, International Union of Crystallography

    Professor Xi Chen

  4. Engineering Amyloid Fibrils from β-Solenoid Proteins for Biomaterials Applications

    Article • ACS Nano, January 2015, American Chemical Society (ACS)

    Professor Xi Chen

  5. Improved one-pot multienzyme (OPME) systems for synthesizing UDP-uronic acids and glucuronides

    Article • Chemical Communications, January 2015, Royal Society of Chemistry

    Professor Xi Chen, Dr Lei Li

  6. Chemoenzymatic synthesis of lacto-N-tetrasaccharide and sialyl lacto-N-tetrasaccharides

    Article • Carbohydrate Research, January 2015, Elsevier

    Professor Xi Chen

  7. Sequential one-pot multienzyme (OPME) synthesis of lacto-N-neotetraose and its sialyl and fucosyl derivatives

    Article • Chemical Communications, January 2015, Royal Society of Chemistry

    Professor Xi Chen

  8. Oral Streptococci Utilize a Siglec-Like Domain of Serine-Rich Repeat Adhesins to Preferentially Target Platelet Sialoglycans in Human Blood

    Article • PLoS Pathogens, December 2014, PLOS

    Professor Xi Chen

  9. Host Adaptation of a Bacterial Toxin from the Human Pathogen Salmonella Typhi

    Article • Cell, December 2014, Elsevier

    Professor Xi Chen

  10. Crystal structures of sialyltransferase from Photobacterium damselae

    Article • FEBS Letters, November 2014, Wiley

    Professor Xi Chen

  11. Broad and direct interaction between TLR and Siglec families of pattern recognition receptors and its regulation by Neu1

    Article • eLife, September 2014, eLife

    Professor Xi Chen, Dr. Alessandra d'Azzo

  12. Synthetic Disialyl Hexasaccharides Protect Neonatal Rats from Necrotizing Enterocolitis

    Article • Angewandte Chemie International Edition, May 2014, Wiley

    Professor Xi Chen

  13. Chemoenzymatic synthesis of sialosides containing C7-modified sialic acids and their application in sialidase substrate specificity studies

    Article • Carbohydrate Research, May 2014, Elsevier

    Professor Xi Chen

  14. Regioselective Chemoenzymatic Synthesis of Ganglioside Disialyl Tetrasaccharide Epitopes

    Article • Journal of the American Chemical Society, April 2014, American Chemical Society (ACS)

    Professor Xi Chen

  15. Profiling of Glycan Receptors for Minute Virus of Mice in Permissive Cell Lines Towards Understanding the Mechanism of Cell Recognition

    Article • PLoS ONE, January 2014, PLOS

    Professor Xi Chen

  16. Highly efficient one-pot multienzyme (OPME) synthesis of glycans with fluorous-tag assisted purification

    Article • Chemical Communications, January 2014, Royal Society of Chemistry

    Professor Xi Chen

  17. Rapid evolution of binding specificities and expression patterns of inhibitory CD33‐related Siglecs in primates

    Article • The FASEB Journal, December 2013, Wiley

    Professor Xi Chen

  18. Structural Basis for Substrate Specificity and Mechanism of N -Acetyl- d -neuraminic Acid Lyase from Pasteurella multocida

    Article • Biochemistry, November 2013, American Chemical Society (ACS)

    Professor Xi Chen

  19. An optics-based variable-temperature assay system for characterizing thermodynamics of biomolecular reactions on solid support

    Article • Review of Scientific Instruments, November 2013, American Institute of Physics

    Professor Xi Chen

  20. Efficient Enzymatic Synthesis of Guanosine 5′-Diphosphate-Sugars and Derivatives

    Article • Organic Letters, November 2013, American Chemical Society (ACS)

    Professor Xi Chen

  21. Tailored Design and Synthesis of Heparan Sulfate Oligosaccharide Analogues Using Sequential One-Pot Multienzyme Systems

    Article • Angewandte Chemie International Edition, September 2013, Wiley

    Professor Xi Chen

  22. Long-Term IgG Response to Porcine Neu5Gc Antigens without Transmission of PERV in Burn Patients Treated with Porcine Skin Xenografts

    Article • The Journal of Immunology, August 2013, The American Association of Immunologists

    Professor Xi Chen

  23. One-pot multi-enzyme (OPME) chemoenzymatic synthesis of sialyl-Tn-MUC1 and sialyl-T-MUC1 glycopeptides containing natural or non-natural sialic acid

    Article • Bioorganic & Medicinal Chemistry, August 2013, Elsevier

    Professor Xi Chen

  24. Exploration of Sialic Acid Diversity and Biology Using Sialoglycan Microarrays

    Article • Biopolymers, July 2013, Wiley

    Professor Xi Chen

  25. Substrate Specificity Provides Insights into the Sugar Donor Recognition Mechanism of O-GlcNAc Transferase (OGT)

    Article • PLoS ONE, May 2013, PLOS

    Professor Xi Chen

  26. Donor substrate promiscuity of the N-acetylglucosaminyltransferase activities of Pasteurella multocida heparosan synthase 2 (PmHS2) and Escherichia coli K5 KfiA

    Article • Applied Microbiology and Biotechnology, May 2013, Springer Science + Business Media

    Professor Xi Chen

  27. Quantum Dot Nanometal Surface Energy Transfer Based Biosensing of Sialic Acid Compositions and Linkages in Biological Samples

    Article • Analytical Chemistry, April 2013, American Chemical Society (ACS)

    Professor Xi Chen

  28. A Simple Method for Assessment of Human Anti-Neu5Gc Antibodies Applied to Kawasaki Disease

    Article • PLoS ONE, March 2013, PLOS

    Professor Xi Chen

  29. Chemoenzymatic synthesis of mono- and di-fluorinated Thomsen–Friedenreich (T) antigens and their sialylated derivatives

    Article • Organic & Biomolecular Chemistry, January 2013, Royal Society of Chemistry

    Professor Xi Chen

  30. One‐Pot Multienzyme Synthesis of Lewis x and Sialyl Lewis x Antigens

    Article • Current Protocols in Chemical Biology, September 2012, Wiley

    Professor Xi Chen

  31. A Sialyltransferase Mutant with Decreased Donor Hydrolysis and Reduced Sialidase Activities for Directly Sialylating Lewis x

    Article • ACS Chemical Biology, July 2012, American Chemical Society (ACS)

    Professor Xi Chen

  32. Structural Features Affecting Trafficking, Processing, and Secretion of Trypanosoma cruzi Mucins

    Article • Journal of Biological Chemistry, June 2012, American Society for Biochemistry & Molecular Biology (ASBMB)

    Professor Xi Chen

  33. Cross-comparison of Protein Recognition of Sialic Acid Diversity on Two Novel Sialoglycan Microarrays

    Article • Journal of Biological Chemistry, May 2012, American Society for Biochemistry & Molecular Biology (ASBMB)

    Professor Xi Chen

  34. Sialic acid metabolism and sialyltransferases: natural functions and applications

    Article • Applied Microbiology and Biotechnology, April 2012, Springer Science + Business Media

    Professor Xi Chen

  35. LC–MS Analysis of Polyclonal Human Anti-Neu5Gc Xeno-Autoantibodies Immunoglobulin G Subclass and Partial Sequence Using Multistep Intravenous Immunoglobulin Affinity Purification and Multienzymatic Digestion

    Article • Analytical Chemistry, March 2012, American Chemical Society (ACS)

    Professor Xi Chen

  36. Efficient one-pot multienzyme synthesis of UDP-sugars using a promiscuous UDP-sugar pyrophosphorylase from Bifidobacterium longum (BLUSP)

    Article • Chemical Communications, January 2012, Royal Society of Chemistry

    Professor Xi Chen

  37. Probe sialidase substrate specificity using chemoenzymatically synthesized sialosides containing C9-modified sialic acid

    Article • Chemical Communications, January 2012, Royal Society of Chemistry

    Professor Xi Chen

  38. Synthesis of selective inhibitors against V. cholerae sialidase and human cytosolic sialidase NEU2

    Article • Organic & Biomolecular Chemistry, January 2012, Royal Society of Chemistry

    Professor Xi Chen

  39. PmST3 from Pasteurella multocida encoded by Pm1174 gene is a monofunctional α2–3-sialyltransferase

    Article • Applied Microbiology and Biotechnology, November 2011, Springer Science + Business Media

    Professor Xi Chen

  40. Pasteurella multocida CMP-sialic acid synthetase and mutants of Neisseria meningitidis CMP-sialic acid synthetase with improved substrate promiscuity

    Article • Applied Microbiology and Biotechnology, October 2011, Springer Science + Business Media

    Professor Xi Chen

  41. Surface Plasmon Resonance Imaging Analysis of Protein Binding to a Sialoside-Based Carbohydrate Microarray

    Article • September 2011, Springer Science + Business Media

    Professor Xi Chen

  42. General Consideration on Sialic Acid Chemistry

    Article • September 2011, Springer Science + Business Media

    Professor Xi Chen

  43. Analysis of Influenza Virus Hemagglutinin Receptor Binding Mutants with Limited Receptor Recognition Properties and Conditional Replication Characteristics

    Article • Journal of Virology, September 2011, ASM Journals

    Professor Xi Chen

  44. Molecular diversity of the Trypanosoma cruzi TcSMUG family of mucin genes and proteins

    Article • Biochemical Journal, September 2011, Portland Press Ltd.

    Professor Xi Chen

  45. Chemoenzymatic synthesis of C8-modified sialic acids and related α2–3- and α2–6-linked sialosides

    Article • Bioorganic & Medicinal Chemistry Letters, September 2011, Elsevier

    Professor Xi Chen

  46. Substrate Promiscuity of N-Acetylhexosamine 1-Kinases

    Article • Molecules, July 2011, MDPI AG

    Professor Xi Chen

  47. A Sialylated Glycan Microarray Reveals Novel Interactions of Modified Sialic Acids with Proteins and Viruses

    Article • Journal of Biological Chemistry, July 2011, American Society for Biochemistry & Molecular Biology (ASBMB)

    Professor Xi Chen

  48. Measurement of Enzymatic Activity and Specificity of Human and Avian Influenza Neuraminidases from Whole Virus by Glycoarray and MALDI-TOF Mass Spectrometry

    Article • ChemBioChem, July 2011, Wiley

    Professor Xi Chen

  49. High-throughput neuraminidase substrate specificity study of human and avian influenza A viruses

    Article • Virology, June 2011, Elsevier

    Professor Xi Chen

  50. PmST2: A novel Pasteurella multocida glycolipid  2-3-sialyltransferase

    Article • Glycobiology, April 2011, Oxford University Press (OUP)

    Professor Xi Chen

  51. Human Xeno-Autoantibodies against a Non-Human Sialic Acid Serve as Novel Serum Biomarkers and Immunotherapeutics in Cancer

    Article • Cancer Research, April 2011, American Association for Cancer Research (AACR)

    Professor Xi Chen

  52. Amelioration of sepsis by inhibiting sialidase-mediated disruption of the CD24-SiglecG interaction

    Article • Nature Biotechnology, April 2011, Springer Science + Business Media

    Professor Xi Chen

  53. An Infant-associated Bacterial Commensal Utilizes Breast Milk Sialyloligosaccharides

    Article • Journal of Biological Chemistry, February 2011, American Society for Biochemistry & Molecular Biology (ASBMB)

    Professor Xi Chen

  54. Fermenting Next Generation Glycosylated Therapeutics

    Article • ACS Chemical Biology, January 2011, American Chemical Society (ACS)

    Professor Xi Chen

  55. Efficient chemoenzymatic synthesis of sialyl Tn-antigens and derivatives

    Article • Chemical Communications, January 2011, Royal Society of Chemistry

    Professor Xi Chen

  56. One-pot three-enzyme synthesis of UDP-GlcNAc derivatives

    Article • Chemical Communications, January 2011, Royal Society of Chemistry

    Professor Xi Chen

  57. Sequential two-step multienzyme synthesis of tumor-associated sialyl T-antigens and derivatives

    Article • Organic & Biomolecular Chemistry, January 2011, Royal Society of Chemistry

    Professor Xi Chen

  58. Identifying selective inhibitors against the human cytosolic sialidase NEU2 by substrate specificity studies

    Article • Molecular BioSystems, January 2011, Royal Society of Chemistry

    Professor Xi Chen

  59. Decreasing the sialidase activity of multifunctional Pasteurella multocidaα2–3-sialyltransferase 1 (PmST1) by site-directed mutagenesis

    Article • Molecular BioSystems, January 2011, Royal Society of Chemistry

    Professor Xi Chen

  60. Fluorescent labeling agents change binding profiles of glycan-binding proteins

    Article • Molecular BioSystems, January 2011, Royal Society of Chemistry

    Professor Xi Chen

  61. Chemoenzymatic synthesis of α2–3-sialylated carbohydrate epitopes

    Article • Science China Chemistry, January 2011, Springer Science + Business Media

    Professor Xi Chen

  62. Cloning and characterization of a viral  2-3-sialyltransferase (vST3Gal-I) for the synthesis of sialyl Lewisx

    Article • Glycobiology, October 2010, Oxford University Press (OUP)

    Professor Xi Chen

  63. Novel mechanism for the generation of human xeno-autoantibodies against the nonhuman sialic acid N -glycolylneuraminic acid

    Article • The Journal of Experimental Medicine, July 2010, Rockefeller University Press

    Professor Xi Chen

  64. Helicobacter hepaticus Hh0072 gene encodes a novel  1-3-fucosyltransferase belonging to CAZy GT11 family

    Article • Glycobiology, May 2010, Oxford University Press (OUP)

    Professor Xi Chen

  65. Identification of glycoproteins targeted by Trypanosoma cruzi trans-sialidase, a virulence factor that disturbs lymphocyte glycosylation

    Article • Glycobiology, March 2010, Oxford University Press (OUP)

    Professor Xi Chen

  66. Advances in the Biology and Chemistry of Sialic Acids

    Article • ACS Chemical Biology, February 2010, American Chemical Society (ACS)

    Professor Xi Chen

  67. Highly efficient chemoenzymatic synthesis of β1–4-linked galactosides with promiscuous bacterial β1–4-galactosyltransferases

    Article • Chemical Communications, January 2010, Royal Society of Chemistry

    Professor Xi Chen

  68. Highly efficient chemoenzymatic synthesis of β1–3-linked galactosides

    Article • Chemical Communications, January 2010, Royal Society of Chemistry

    Professor Xi Chen

  69. Chemoenzymatic Synthesis of GD3 Oligosaccharides and Other Disialyl Glycans Containing Natural and Non-natural Sialic Acids

    Article • Journal of the American Chemical Society, December 2009, American Chemical Society (ACS)

    Professor Xi Chen

  70. Recent progress in chemical and chemoenzymatic synthesis of carbohydrates

    Article • Current Opinion in Chemical Biology, December 2009, Elsevier

    Professor Xi Chen

  71. Trans-sialidase activity of Photobacterium damsela  2,6-sialyltransferase and its application in the synthesis of sialosides

    Article • Glycobiology, October 2009, Oxford University Press (OUP)

    Professor Xi Chen

  72. Evidence for a novel human-specific xeno-auto-antibody response against vascular endothelium

    Article • Blood, October 2009, American Society of Hematology

    Professor Xi Chen

  73. Parallel chemoenzymatic synthesis of sialosides containing a C5-diversified sialic acid

    Article • Bioorganic & Medicinal Chemistry Letters, October 2009, Elsevier

    Professor Xi Chen

  74. Fabrication and Characterization of a Sialoside-Based Carbohydrate Microarray Biointerface for Protein Binding Analysis with Surface Plasmon Resonance Imaging

    Article • ACS Applied Materials & Interfaces, August 2009, American Chemical Society (ACS)

    Professor Xi Chen

  75. Chemoenzymatic Synthesis of a New Class of Macrocyclic Oligosaccharides

    Article • The Journal of Organic Chemistry, April 2009, American Chemical Society (ACS)

    Professor Xi Chen

  76. Remodeling bacterial polysaccharides by metabolic pathway engineering

    Article • Proceedings of the National Academy of Sciences, March 2009, Proceedings of the National Academy of Sciences

    Professor Xi Chen

  77. Sensitive and Specific Detection of the Non-Human Sialic Acid N-Glycolylneuraminic Acid In Human Tissues and Biotherapeutic Products

    Article • PLoS ONE, January 2009, PLOS

    Professor Xi Chen

  78. Sialidase substrate specificity studies using chemoenzymatically synthesized sialosides containing C5-modified sialic acids

    Article • Organic & Biomolecular Chemistry, January 2009, Royal Society of Chemistry

    Professor Xi Chen

  79. Synthesis of sulfone-based nucleotide isosteres: identification of CMP-sialic acid synthetase inhibitors

    Article • Organic & Biomolecular Chemistry, January 2009, Royal Society of Chemistry

    Professor Xi Chen

  80. Incorporation of a non-human glycan mediates human susceptibility to a bacterial toxin

    Article • Nature, December 2008, Springer Science + Business Media

    Professor Xi Chen, Dr Travis Beddoe

  81. Chemical preparation of sialyl Lewis x using an enzymatically synthesized sialoside building block

    Article • Carbohydrate Research, November 2008, Elsevier

    Professor Xi Chen

  82. Combinatorial Chemoenzymatic Synthesis and High-Throughput Screening of Sialosides

    Article • ACS Chemical Biology, September 2008, American Chemical Society (ACS)

    Professor Xi Chen

  83. Chemoenzymatic Syntheses of Tumor‐Associated Carbohydrate Antigen Globo‐H and Stage‐Specific Embryonic Antigen 4

    Article • Advanced Synthesis & Catalysis, July 2008, Wiley

    Dr Xuefei Huang, Professor Xi Chen

  84. Pasteurella multocida sialic acid aldolase: a promising biocatalyst

    Article • Applied Microbiology and Biotechnology, July 2008, Springer Science + Business Media

    Professor Xi Chen

  85. Multifunctionality of Campylobacter jejuni sialyltransferase CstII: Characterization of GD3/GT3 oligosaccharide synthase, GD3 oligosaccharide sialidase, and trans-sialidase activities

    Article • Glycobiology, June 2008, Oxford University Press (OUP)

    Professor Xi Chen

  86. Diversity in specificity, abundance, and composition of anti-Neu5Gc antibodies in normal humans: Potential implications for disease

    Article • Glycobiology, June 2008, Oxford University Press (OUP)

    Professor Xi Chen

  87. Surface Plasmon Resonance Study of Protein−Carbohydrate Interactions Using Biotinylated Sialosides

    Article • Analytical Chemistry, June 2008, American Chemical Society (ACS)

    Professor Xi Chen

  88. Chemoenzymatic Synthesis of Size-Defined Polysaccharides by Sialyltransferase-Catalyzed Block Transfer of Oligosaccharides

    Article • Journal of the American Chemical Society, October 2007, American Chemical Society (ACS)

    Professor Xi Chen

  89. The Hd0053 gene of Haemophilus ducreyi encodes an α2,3-sialyltransferase

    Article • Biochemical and Biophysical Research Communications, September 2007, Elsevier

    Professor Xi Chen

  90. Enzymatic Synthesis of Fluorinated Mechanistic Probes for Sialidases and Sialyltransferases

    Article • Journal of the American Chemical Society, September 2007, American Chemical Society (ACS)

    Professor Xi Chen

  91. NeuA Sialic Acid O-Acetylesterase Activity Modulates O-Acetylation of Capsular Polysaccharide in Group B Streptococcus

    Article • Journal of Biological Chemistry, July 2007, American Society for Biochemistry & Molecular Biology (ASBMB)

    Professor Xi Chen

  92. Crystal Structures of Pasteurella multocida Sialyltransferase Complexes with Acceptor and Donor Analogues Reveal Substrate Binding Sites and Catalytic Mechanism † , ‡

    Article • Biochemistry, May 2007, American Chemical Society (ACS)

    Professor Xi Chen

  93. Disaccharides as Sialic Acid Aldolase Substrates: Synthesis of Disaccharides Containing a Sialic Acid at the Reducing End

    Article • Angewandte Chemie International Edition, March 2007, Wiley

    Professor Xi Chen

  94. High-Throughput Substrate Specificity Studies of Sialidases by Using Chemoenzymatically Synthesized Sialoside Libraries

    Article • ChemBioChem, January 2007, Wiley

    Professor Xi Chen

  95. Efficient chemoenzymatic synthesis of biotinylated human serum albumin–sialoglycoside conjugates containing O-acetylated sialic acids

    Article • Organic & Biomolecular Chemistry, January 2007, Royal Society of Chemistry

    Professor Xi Chen

  96. Carbohydrate post-glycosylational modifications

    Article • Organic & Biomolecular Chemistry, January 2007, Royal Society of Chemistry

    Professor Xi Chen

  97. One-pot three-enzyme chemoenzymatic approach to the synthesis of sialosides containing natural and non-natural functionalities

    Article • Nature Protocols, December 2006, Springer Science + Business Media

    Professor Xi Chen

  98. Highly Efficient Chemoenzymatic Synthesis of Naturally Occurring and Non-Natural α-2,6-Linked Sialosides: AP. damsela α-2,6-Sialyltransferase with Extremely Flexible Donor–Substrate Specificity

    Article • Angewandte Chemie International Edition, June 2006, Wiley

    Professor Xi Chen

  99. Aldolase-Catalyzed Synthesis of β- d -Gal p -(1→9)- d -KDN: A Novel Acceptor for Sialyltransferases

    Article • Organic Letters, May 2006, American Chemical Society (ACS)

    Professor Xi Chen

  100. Cytidine 5‘-Monophosphate (CMP)-Induced Structural Changes in a Multifunctional Sialyltransferase from Pasteurella multocida † , ‡

    Article • Biochemistry, February 2006, American Chemical Society (ACS)

    Professor Xi Chen

  101. Characterization of a Bifunctional Cytidine 5′-Monophosphate N-acetylneuraminic acid Synthetase Cloned from Streptococcus Agalactiae

    Article • Biotechnology Letters, January 2006, Springer Science + Business Media

    Professor Xi Chen

  102. A Multifunctional Pasteurella multocida Sialyltransferase:  A Powerful Tool for the Synthesis of Sialoside Libraries

    Article • Journal of the American Chemical Society, December 2005, American Chemical Society (ACS)

    Professor Xi Chen

  103. Chemoenzymatic synthesis of CMP–sialic acid derivatives by a one-pot two-enzyme system: comparison of substrate flexibility of three microbial CMP–sialic acid synthetases

    Article • Bioorganic & Medicinal Chemistry, December 2004, Elsevier

    Professor Xi Chen