All Stories

  1. The Photocycle of the Red Light-Sensitive Plant-like Cryptochrome CryP from Phaeodactylum tricornutum

    Article • Biochemistry, August 2026, American Chemical Society (ACS)

    Prof. Dr. Tilman Kottke

  2. The Human Circadian Clock Protein Cryptochrome-1 Responds to Photochemistry by Conformational Changes

    Article • Journal of the American Chemical Society, August 2026, American Chemical Society (ACS)

    Prof. Dr. Tilman Kottke

  3. Partial Conversion of an Animal-Like Cryptochrome into a Plant Cryptochrome

    Article • The Journal of Physical Chemistry, February 2026, American Chemical Society (ACS)

    Prof. Dr. Tilman Kottke

  4. Comprehensive optical spectroscopy of solid-state polymer electrolytes: Harmonic and anharmonic vibrational activity and charge dynamics from terahertz to ultraviolet

    Article • The Journal of Chemical Physics, October 2025, American Institute of Physics

    Prof. Dr. Tilman Kottke

  5. Constitutively active Arabidopsis cryptochrome 2 alleles identified using yeast selection and deep mutational scanning

    Article • Journal of Biological Chemistry, June 2025, Elsevier

    Prof. Dr. Tilman Kottke

  6. Photochemistry of Receptor-Bound Flavin Resolved in Living Human Cells by Infrared Spectroscopy

    Article • Journal of the American Chemical Society, March 2025, American Chemical Society (ACS)

    Prof. Dr. Tilman Kottke

  7. The NADH-dependent flavin reductase ThdF follows an ordered sequential mechanism though crystal structures reveal two FAD molecules in the active site

    Article • Journal of Biological Chemistry, February 2025, Elsevier

    Prof. Dr. Tilman Kottke

  8. Balance between photoreduction efficiency, cofactor affinity, and allosteric coupling of halogenase flavoenzymes

    Article • Photochemical & Photobiological Sciences, December 2024, Springer Science + Business Media

    Prof. Dr. Tilman Kottke

  9. Coupling and regulation mechanisms of the flavin-dependent halogenase PyrH observed by infrared difference spectroscopy

    Article • Journal of Biological Chemistry, April 2024, Elsevier

    Prof. Dr. Tilman Kottke

  10. Light-induced Trpin/Metout Switching During BLUF Domain Activation in ATP-bound Photoactivatable Adenylate Cyclase OaPAC

    Article • Journal of Molecular Biology, March 2024, Elsevier

    Jayanath Koliyadu, Prof. Dr. Tilman Kottke

  11. Time-resolved infrared difference spectroscopy in cells: Response of the basic region leucine zipper of aureochrome

    Article • Frontiers in Physics, March 2023, Frontiers

    Prof. Dr. Tilman Kottke

  12. Genetically Encoded Ratiometric pH Sensors for the Measurement of Intra- and Extracellular pH and Internalization Rates

    Article • Biosensors, April 2022, MDPI AG

    Prof. Dr. Tilman Kottke

  13. Core–shell microgels synthesized in continuous flow: deep insight into shell growth using temperature-dependent FTIR

    Article • Soft Matter, January 2022, Royal Society of Chemistry

    Prof. Dr. Tilman Kottke

  14. Plant Cryptochromes Illuminated: A Spectroscopic Perspective on the Mechanism

    Article • Frontiers in Chemistry, November 2021, Frontiers

    Prof. Dr. Tilman Kottke

  15. The World of Algae Reveals a Broad Variety of Cryptochrome Properties and Functions

    Article • Frontiers in Plant Science, November 2021, Frontiers

    Prof. Dr. Tilman Kottke

  16. DASH cryptochrome 1, a UV‐A receptor, balances the photosynthetic machinery of Chlamydomonas reinhardtii

    Article • New Phytologist, July 2021, Wiley

    Prof. Dr. Tilman Kottke

  17. C-Terminal Extension of a Plant Cryptochrome Dissociates from the β-Sheet of the Flavin-Binding Domain

    Article • The Journal of Physical Chemistry Letters, June 2021, American Chemical Society (ACS)

    Prof. Dr. Tilman Kottke

  18. Smart Microgels from Unconventional Acrylamides

    Article • Macromolecular Chemistry and Physics, May 2021, Wiley

    Prof. Dr. Tilman Kottke

  19. Peripheral Methionine Residues Impact Flavin Photoreduction and Protonation in an Engineered LOV Domain Light Sensor

    Article • Biochemistry, March 2021, American Chemical Society (ACS)

    Prof. Dr. Tilman Kottke

  20. Resolving Structural Changes of Photoreceptors in Living Escherichia coli via In-cell Infrared Difference Spectroscopy

    Article • BIO-PROTOCOL, January 2021, Bio-Protocol, LLC

    Prof. Dr. Tilman Kottke

  21. Site-by-site tracking of signal transduction in an azidophenylalanine-labeled bacteriophytochrome with step-scan FTIR spectroscopy

    Article • Physical Chemistry Chemical Physics, January 2021, Royal Society of Chemistry

    Prof. Dr. Tilman Kottke

  22. Smart membranes by electron beam cross-linking of copolymer microgels

    Article • Soft Matter, January 2021, Royal Society of Chemistry

    Prof. Dr. Tilman Kottke

  23. Acrylamide precipitation polymerization in a continuous flow reactor: an in situ FTIR study reveals kinetics

    Article • Colloid & Polymer Science, November 2020, Springer Science + Business Media

    Prof. Dr. Tilman Kottke

  24. Tailored flavoproteins acting as light-driven spin machines pump nuclear hyperpolarization

    Article • Scientific Reports, October 2020, Springer Science + Business Media

    Prof. Dr. Tilman Kottke

  25. In-cell infrared difference spectroscopy of LOV photoreceptors reveals structural responses to light altered in living cells

    Article • Journal of Biological Chemistry, August 2020, Elsevier

    Prof. Dr. Tilman Kottke

  26. Tongue Refolding in the Knotless Cyanobacterial Phytochrome All2699

    Article • Biochemistry, May 2020, American Chemical Society (ACS)

    Prof. Dr. Tilman Kottke

  27. Calculation of the Geometries and Infrared Spectra of the Stacked Cofactor Flavin Adenine Dinucleotide (FAD) as the Prerequisite for Studies of Light-Triggered Proton and Electron Transfer

    Article • Biomolecules, April 2020, MDPI AG

    Prof. Dr. Tilman Kottke

  28. A quantum cascade laser setup for studying irreversible photoreactions in H2O with nanosecond resolution and microlitre consumption

    Article • Physical Chemistry Chemical Physics, January 2020, Royal Society of Chemistry

    Prof. Dr. Tilman Kottke

  29. Core–shell microgels as thermoresponsive carriers for catalytic palladium nanoparticles

    Article • Soft Matter, January 2020, Royal Society of Chemistry

    Prof. Dr. Tilman Kottke

  30. Nuclear spin-hyperpolarization generated in a flavoprotein under illumination: experimental field-dependence and theoretical level crossing analysis

    Article • Scientific Reports, December 2019, Springer Science + Business Media

    Prof. Dr. Tilman Kottke

  31. Time-Resolved Infrared and Visible Spectroscopy on Cryptochrome aCRY: Basis for Red Light Reception

    Article • Biophysical Journal, August 2019, Elsevier

    Prof. Dr. Tilman Kottke

  32. Biphasic Formation of 2D Nanomembranes by Photopolymerization of Diacetylene Lipids as Revealed by Infrared Difference Spectroscopy

    Article • Langmuir, June 2019, American Chemical Society (ACS)

    Prof. Dr. Tilman Kottke

  33. Arguments for an additional long-lived intermediate in the photocycle of the full-length aureochrome 1c receptor: A time-resolved small-angle X-ray scattering study

    Article • Structural Dynamics, May 2019, American Institute of Physics

    Prof. Dr. Tilman Kottke

  34. Straightforward Regeneration of Reduced Flavin Adenine Dinucleotide Required for Enzymatic Tryptophan Halogenation

    Article • ACS Catalysis, January 2019, American Chemical Society (ACS)

    Prof. Dr. Tilman Kottke

  35. Aurophilicity in action: stepwise formation of dinuclear Au(i) macrocycles with rigid 1,8-dialkynylanthracenes

    Article • Dalton Transactions, January 2019, Royal Society of Chemistry

    Prof. Dr. Tilman Kottke

  36. Following local light-induced structure changes and dynamics of the photoreceptor PYP with the thiocyanate IR label

    Article • Physical Chemistry Chemical Physics, January 2019, Royal Society of Chemistry

    Prof. Dr. Tilman Kottke

  37. Swelling behaviour of core–shell microgels in H2O, analysed by temperature-dependent FTIR spectroscopy

    Article • Physical Chemistry Chemical Physics, January 2019, Royal Society of Chemistry

    Prof. Dr. Tilman Kottke

  38. Synthesis of smart dual-responsive microgels: correlation between applied surfactants and obtained particle morphology

    Article • Soft Matter, January 2019, Royal Society of Chemistry

    Prof. Dr. Tilman Kottke

  39. Chromophore–Protein Interplay during the Phytochrome Photocycle Revealed by Step-Scan FTIR Spectroscopy

    Article • Journal of the American Chemical Society, September 2018, American Chemical Society (ACS)

    Prof. Dr. Tilman Kottke

  40. Single-Shot Sub-microsecond Mid-infrared Spectroscopy on Protein Reactions with Quantum Cascade Laser Frequency Combs

    Article • Analytical Chemistry, August 2018, American Chemical Society (ACS)

    Prof. Dr. Tilman Kottke

  41. Photochemically Driven Biocatalysis of Halogenases for the Green Production of Chlorinated Compounds

    Article • ChemCatChem, May 2018, Wiley

    Prof. Dr. Tilman Kottke

  42. Photoreceptors Take Charge: Emerging Principles for Light Sensing

    Article • Annual Review of Biophysics, May 2018, Annual Reviews

    Prof. Dr. Tilman Kottke

  43. A flavin-dependent halogenase from metagenomic analysis prefers bromination over chlorination

    Article • PLOS One, May 2018, PLOS

    Prof. Dr. Tilman Kottke

  44. Characterization of Robust and Free-Standing 2D-Nanomembranes of UV-Polymerized Diacetylene Lipids

    Article • Langmuir, February 2018, American Chemical Society (ACS)

    Prof. Dr. Tilman Kottke

  45. Time-Resolved Infrared Spectroscopy on Plant Cryptochrome—Relevance of Proton Transfer and ATP Binding for Signaling

    Article • The Journal of Physical Chemistry A, December 2017, American Chemical Society (ACS)

    Prof. Dr. Tilman Kottke

  46. Activation of Recombinantly Expressed l-Amino Acid Oxidase from Rhizoctonia solani by Sodium Dodecyl Sulfate

    Article • Molecules, December 2017, MDPI AG

    Prof. Dr. Tilman Kottke

  47. An update on aureochromes: Phylogeny – mechanism – function

    Article • Journal of Plant Physiology, October 2017, Elsevier

    Prof. Dr. Tilman Kottke

  48. Cryptochrome photoreceptors in green algae: Unexpected versatility of mechanisms and functions

    Article • Journal of Plant Physiology, October 2017, Elsevier

    Prof. Dr. Tilman Kottke

  49. The cryptochrome—photolyase protein family in diatoms

    Article • Journal of Plant Physiology, October 2017, Elsevier

    Prof. Dr. Tilman Kottke

  50. Microphase separation of smart double-responsive copolymer microgels studied by local fluorescence probes

    Article • Polymer, September 2017, Elsevier

    Prof. Dr. Tilman Kottke

  51. Phosphorus and nitrogen starvation reveal life‐cycle specific responses in the metabolome of Emiliania huxleyi (Haptophyta)

    Article • Limnology and Oceanography, July 2017, Wiley

    Prof. Dr. Tilman Kottke

  52. An Animal-Like Cryptochrome Controls the Chlamydomonas Sexual Cycle

    Article • PLANT PHYSIOLOGY, May 2017, Oxford University Press (OUP)

    Prof. Dr. Tilman Kottke

  53. Light-Induced Conformational Changes in the Plant Cryptochrome Photolyase Homology Region Resolved by Selective Isotope Labeling and Infrared Spectroscopy

    Article • Photochemistry and Photobiology, May 2017, Wiley

    Prof. Dr. Tilman Kottke

  54. A Plant Cryptochrome Controls Key Features of the Chlamydomonas Circadian Clock and Its Life Cycle

    Article • PLANT PHYSIOLOGY, March 2017, Oxford University Press (OUP)

    Prof. Dr. Tilman Kottke

  55. Boreal pollen contain ice-nucleating as well as ice-binding ‘antifreeze’ polysaccharides

    Article • Scientific Reports, February 2017, Springer Science + Business Media

    Prof. Dr. Tilman Kottke

  56. Recombinant expression and characterization of a l-amino acid oxidase from the fungus Rhizoctonia solani

    Article • Applied Microbiology and Biotechnology, December 2016, Springer Science + Business Media

    Prof. Dr. Tilman Kottke

  57. The Grateful Infrared: Sequential Protein Structural Changes Resolved by Infrared Difference Spectroscopy

    Article • The Journal of Physical Chemistry, December 2016, American Chemical Society (ACS)

    Prof. Dr. Tilman Kottke

  58. A blue-light photoreceptor mediates the feedback regulation of photosynthesis

    Article • Nature, September 2016, Springer Science + Business Media

    Prof. Dr. Tilman Kottke

  59. Essential Role of an Unusually Long-lived Tyrosyl Radical in the Response to Red Light of the Animal-like Cryptochrome aCRY

    Article • Journal of Biological Chemistry, July 2016, Elsevier

    Prof. Dr. Tilman Kottke

  60. Allosteric communication between DNA-binding and light-responsive domains of diatom class I aureochromes

    Article • Nucleic Acids Research, May 2016, Oxford University Press (OUP)

    Dr Ankan Banerjee, Prof. Dr. Tilman Kottke

  61. Evidence for Tautomerisation of Glutamine in BLUF Blue Light Receptors by Vibrational Spectroscopy and Computational Chemistry

    Article • Scientific Reports, March 2016, Springer Science + Business Media

    Prof. Dr. Tilman Kottke

  62. Structure of a Native-like Aureochrome 1a LOV Domain Dimer from Phaeodactylum tricornutum

    Article • Structure, January 2016, Elsevier

    Dr Ankan Banerjee, Prof. Dr. Tilman Kottke

  63. Microsecond Deprotonation of Aspartic Acid and Response of the α/β Subdomain Precede C-Terminal Signaling in the Blue Light Sensor Plant Cryptochrome

    Article • Journal of the American Chemical Society, May 2015, American Chemical Society (ACS)

    Prof. Dr. Tilman Kottke

  64. Allosterically Regulated Unfolding of the A′α Helix Exposes the Dimerization Site of the Blue-Light-Sensing Aureochrome-LOV Domain

    Article • Biochemistry, February 2015, American Chemical Society (ACS)

    Prof. Dr. Tilman Kottke

  65. Proton Transfer to Flavin Stabilizes the Signaling State of the Blue Light Receptor Plant Cryptochrome

    Article • Journal of Biological Chemistry, January 2015, Elsevier

    Prof. Dr. Tilman Kottke

  66. A novel cryptochrome in the diatom Phaeodactylum tricornutum influences the regulation of light‐harvesting protein levels

    Article • FEBS Journal, April 2014, Wiley

    Prof. Dr. Tilman Kottke

  67. Response of the Sensory Animal-like Cryptochrome aCRY to Blue and Red Light As Revealed by Infrared Difference Spectroscopy

    Article • Biochemistry, February 2014, American Chemical Society (ACS)

    Prof. Dr. Tilman Kottke

  68. Blue-Light-Induced Unfolding of the Jα Helix Allows for the Dimerization of Aureochrome-LOV from the Diatom Phaeodactylum tricornutum

    Article • Biochemistry, April 2013, American Chemical Society (ACS)

    Prof. Dr. Tilman Kottke

  69. News about cryptochrome photoreceptors in algae

    Article • Plant Signaling & Behavior, February 2013, Taylor & Francis

    Prof. Dr. Tilman Kottke

  70. Protonated triplet-excited flavin resolved by step-scan FTIR spectroscopy: implications for photosensory LOV domains

    Article • Physical Chemistry Chemical Physics, January 2013, Royal Society of Chemistry

    Prof. Dr. Tilman Kottke

  71. Single Amino Acid Substitution Reveals Latent Photolyase Activity in Arabidopsis cry1

    Article • Angewandte Chemie, August 2012, Wiley

    Prof. Dr. Tilman Kottke

  72. Primary Events in the Blue Light Sensor Plant Cryptochrome: Intraprotein Electron and Proton Transfer Revealed by Femtosecond Spectroscopy

    Article • Journal of the American Chemical Society, July 2012, American Chemical Society (ACS)

    Prof. Dr. Tilman Kottke

  73. A Flavin Binding Cryptochrome Photoreceptor Responds to Both Blue and Red Light in Chlamydomonas reinhardtii

    Article • The Plant Cell, July 2012, Oxford University Press (OUP)

    Prof. Dr. Tilman Kottke

  74. Infrared spectrum and absorption coefficient of the cofactor flavin in water

    Article • Vibrational Spectroscopy, November 2011, Elsevier

    Prof. Dr. Tilman Kottke

  75. Indication for a Radical Intermediate Preceding the Signaling State in the LOV Domain Photocycle†

    Article • Photochemistry and Photobiology, February 2011, Wiley

    Prof. Dr. Tilman Kottke

  76. Die große Ausnahme: flavinhaltige Blaulichtsensoren

    Article • Nachrichten aus Chemie und Technik, December 2010, Wiley

    Prof. Dr. Tilman Kottke

  77. Photoreaction of Plant and DASH Cryptochromes Probed by Infrared Spectroscopy: The Neutral Radical State of Flavoproteins

    Article • The Journal of Physical Chemistry, December 2010, American Chemical Society (ACS)

    Prof. Dr. Tilman Kottke

  78. Solid-State Photo-CIDNP Effect Observed in Phototropin LOV1-C57S by 13C Magic-Angle Spinning NMR Spectroscopy

    Article • Journal of the American Chemical Society, October 2010, American Chemical Society (ACS)

    Prof. Dr. Tilman Kottke

  79. Identification of the Product of Photoswitching of an Oxazine Fluorophore Using Fourier Transform Infrared Difference Spectroscopy

    Article • The Journal of Physical Chemistry Letters, October 2010, American Chemical Society (ACS)

    Prof. Dr. Tilman Kottke

  80. Thinner, Smaller, Faster: IR Techniques To Probe the Functionality of Biological and Biomimetic Systems

    Article • Angewandte Chemie, July 2010, Wiley

    Prof. Dr. Tilman Kottke

  81. Blue Light Induces Global and Localized Conformational Changes in the Kinase Domain of Full-Length Phototropin

    Article • Biochemistry, January 2010, American Chemical Society (ACS)

    Tilo Mathes, Prof. Dr. Tilman Kottke

  82. Synthesis of Monodisperse Oligo(1,4-phenyleneethynylene-alt-1,4-triptycyleneethynylene)s

    Article • The Journal of Organic Chemistry, September 2009, American Chemical Society (ACS)

    Prof. Dr. Tilman Kottke

  83. Microsecond Light-Induced Proton Transfer to Flavin in the Blue Light Sensor Plant Cryptochrome

    Article • Journal of the American Chemical Society, September 2009, American Chemical Society (ACS)

    Prof. Dr. Tilman Kottke

  84. Time-Resolved Fourier Transform Infrared Study on Photoadduct Formation and Secondary Structural Changes within the Phototropin LOV Domain

    Article • Biophysical Journal, February 2009, Elsevier

    Prof. Dr. Tilman Kottke

  85. Tailoring the properties and the reactivity of the spinel cobalt oxide

    Article • Physical Chemistry Chemical Physics, January 2009, Royal Society of Chemistry

    Prof. Dr. Tilman Kottke

  86. Time‐Resolved FT‐IR Spectroscopy Traces Signal Relay within the Blue‐Light Receptor AppA

    Article • ChemPhysChem, August 2007, Wiley

    Prof. Dr. Tilman Kottke

  87. Blue Light Induces Radical Formation and Autophosphorylation in the Light-sensitive Domain of Chlamydomonas Cryptochrome

    Article • Journal of Biological Chemistry, July 2007, Elsevier

    Prof. Dr. Tilman Kottke

  88. A Novel Photoreaction Mechanism for the Circadian Blue Light Photoreceptor Drosophila Cryptochrome

    Article • Journal of Biological Chemistry, April 2007, Elsevier

    Prof. Dr. Tilman Kottke

  89. The photochemistry of the light‐, oxygen‐, and voltage‐sensitive domains in the algal blue light receptor phot

    Article • Biopolymers, March 2006, Wiley

    Prof. Dr. Tilman Kottke

  90. Blue-Light-Induced Changes in Arabidopsis Cryptochrome 1 Probed by FTIR Difference Spectroscopy

    Article • Biochemistry, February 2006, American Chemical Society (ACS)

    Prof. Dr. Tilman Kottke

  91. The Phot LOV2 Domain and Its Interaction with LOV1

    Article • Biophysical Journal, July 2005, Elsevier

    Prof. Dr. Tilman Kottke

  92. Recording of Blue Light-Induced Energy and Volume Changes within the Wild-Type and Mutated Phot-LOV1 Domain from Chlamydomonas reinhardtii

    Article • Biophysical Journal, February 2004, Elsevier

    Prof. Dr. Tilman Kottke

  93. Irreversible Photoreduction of Flavin in a Mutated Phot-LOV1 Domain

    Article • Biochemistry, August 2003, American Chemical Society (ACS)

    Prof. Dr. Tilman Kottke

  94. Phot-LOV1: Photocycle of a Blue-Light Receptor Domain from the Green Alga Chlamydomonas reinhardtii

    Article • Biophysical Journal, February 2003, Elsevier

    Prof. Dr. Tilman Kottke