All Stories

  1. Pilot-scale development of core-shell polymer supports for the immobilization of recombinant lipase B from Candida antarctica and their application in the production of ethyl esters from residual fatty acids

    Article • Journal of Applied Polymer Science, August 2018, Wiley

    Dr ROBERTO FERNANDEZ-LAFUENTE

  2. Enzymatic synthesis of ethyl esters from waste oil using mixtures of lipases in a plug-flow packed-bed continuous reactor

    Article • Biotechnology Progress, July 2018, Wiley

    Dr ROBERTO FERNANDEZ-LAFUENTE

  3. 1,3-Regiospecific ethanolysis of soybean oil catalyzed by crosslinked porcine pancreas lipase aggregates

    Article • Biotechnology Progress, May 2018, Wiley

    Dr ROBERTO FERNANDEZ-LAFUENTE

  4. Use of Lecitase-Ultra immobilized on styrene-divinylbenzene beads as catalyst of esterification reactions: Effects of ultrasounds

    Article • Catalysis Today, October 2015, Elsevier

    Dr ROBERTO FERNANDEZ-LAFUENTE

  5. Enzymatic reactors for biodiesel synthesis: Present status and future prospects

    Article • Biotechnology Advances, September 2015, Elsevier

    Dr ROBERTO FERNANDEZ-LAFUENTE

  6. Immobilization of Proteins in Poly-Styrene-Divinylbenzene Matrices: Functional Properties and Applications

    Article • Current Organic Chemistry, August 2015, Bentham Science Publishers

    Dr ROBERTO FERNANDEZ-LAFUENTE

  7. Immobilization of lipases on hydrophobic supports involves the open form of the enzyme

    Article • Enzyme and Microbial Technology, April 2015, Elsevier

    Dr ROBERTO FERNANDEZ-LAFUENTE

  8. Improved performance of lipases immobilized on heterofunctional octyl-glyoxyl agarose beads

    Article • RSC Advances, January 2015, Royal Society of Chemistry

    Dr ROBERTO FERNANDEZ-LAFUENTE

  9. Characterization of supports activated with divinyl sulfone as a tool to immobilize and stabilize enzymes via multipoint covalent attachment. Application to chymotrypsin

    Article • RSC Advances, January 2015, Royal Society of Chemistry

    Dr ROBERTO FERNANDEZ-LAFUENTE

  10. Optimization of ethyl ester production from olive and palm oils using mixtures of immobilized lipases

    Article • Applied Catalysis A General, January 2015, Elsevier

    Dr ROBERTO FERNANDEZ-LAFUENTE

  11. Versatility of divinylsulfone supports permits the tuning of CALB properties during its immobilization

    Article • RSC Advances, January 2015, Royal Society of Chemistry

    Dr ROBERTO FERNANDEZ-LAFUENTE

  12. Optimization and characterization of CLEAs of the very thermostable dimeric peroxidase from Roystonea regia

    Article • RSC Advances, January 2015, Royal Society of Chemistry

    Dr ROBERTO FERNANDEZ-LAFUENTE

  13. Reactivation of lipases by the unfolding and refolding of covalently immobilized biocatalysts

    Article • RSC Advances, January 2015, Royal Society of Chemistry

    Dr ROBERTO FERNANDEZ-LAFUENTE

  14. Editorial: Special Issue — Enzyme Immobilization

    Article • Molecules, December 2014, MDPI AG

    Dr ROBERTO FERNANDEZ-LAFUENTE

  15. ChemInform Abstract: Amination of Enzymes to Improve Biocatalyst Performance: Coupling Genetic Modification and Physicochemical Tools

    Article • ChemInform, November 2014, Wiley

    Dr ROBERTO FERNANDEZ-LAFUENTE

  16. Stabilizing hyperactivated lecitase structures through physical treatment with ionic polymers

    Article • Process Biochemistry, September 2014, Elsevier

    Dr ROBERTO FERNANDEZ-LAFUENTE

  17. Combined Effects of Ultrasound and Immobilization Protocol on Butyl Acetate Synthesis Catalyzed by CALB

    Article • Molecules, July 2014, MDPI AG

    Dr ROBERTO FERNANDEZ-LAFUENTE

  18. Chemical modification of lipase B from Candida antarctica for improving biochemical properties of activity, stability and selectivity

    Article • New Biotechnology, July 2014, Elsevier

    Dr ROBERTO FERNANDEZ-LAFUENTE

  19. Comparison of the performance of commercial immobilized lipases in the synthesis of different flavor esters

    Article • Journal of Molecular Catalysis B Enzymatic, July 2014, Elsevier

    Dr ROBERTO FERNANDEZ-LAFUENTE

  20. Evaluation of Styrene-Divinylbenzene Beads as a Support to Immobilize Lipases

    Article • Molecules, June 2014, MDPI AG

    Dr ROBERTO FERNANDEZ-LAFUENTE

  21. Improving the catalytic properties of immobilized Lecitase via physical coating with ionic polymers

    Article • Enzyme and Microbial Technology, June 2014, Elsevier

    Dr ROBERTO FERNANDEZ-LAFUENTE

  22. Antimicrobial Peptides: Promising Compounds Against Pathogenic Microorganisms

    Article • Current Medicinal Chemistry, May 2014, Bentham Science Publishers

    Dr ROBERTO FERNANDEZ-LAFUENTE

  23. Preparation of core–shell polymer supports to immobilize lipase B from Candida antarctica

    Article • Journal of Molecular Catalysis B Enzymatic, February 2014, Elsevier

    Dr ROBERTO FERNANDEZ-LAFUENTE

  24. Design and activity of novel lactoferrampin analogues against O157:H7 enterohemorrhagicescherichia coli

    Article • Biopolymers, January 2014, Wiley

    Dr ROBERTO FERNANDEZ-LAFUENTE

  25. Glutaraldehyde in bio-catalysts design: a useful crosslinker and a versatile tool in enzyme immobilization

    Article • RSC Advances, January 2014, Royal Society of Chemistry

    Dr ROBERTO FERNANDEZ-LAFUENTE

  26. Ultrasound technology and molecular sieves improve the thermodynamically controlled esterification of butyric acid mediated by immobilized lipase from Rhizomucor miehei

    Article • RSC Advances, January 2014, Royal Society of Chemistry

    Dr ROBERTO FERNANDEZ-LAFUENTE

  27. Tuning lipase B from Candida antarctica C–C bond promiscuous activity by immobilization on poly-styrene-divinylbenzene beads

    Article • RSC Advances, January 2014, Royal Society of Chemistry

    Dr ROBERTO FERNANDEZ-LAFUENTE

  28. Combi-lipase for heterogeneous substrates: a new approach for hydrolysis of soybean oil using mixtures of biocatalysts

    Article • RSC Advances, January 2014, Royal Society of Chemistry

    Dr ROBERTO FERNANDEZ-LAFUENTE

  29. Amination of enzymes to improve biocatalyst performance: coupling genetic modification and physicochemical tools

    Article • RSC Advances, January 2014, Royal Society of Chemistry

    Dr ROBERTO FERNANDEZ-LAFUENTE

  30. Efficient purification-immobilization of an organic solvent-tolerant lipase from Staphylococcus warneri EX17 on porous styrene-divinylbenzene beads

    Article • Journal of Molecular Catalysis B Enzymatic, January 2014, Elsevier

    Dr ROBERTO FERNANDEZ-LAFUENTE

  31. Optimization of synthesis of fatty acid methyl esters catalyzed by lipase B from Candida antarctica immobilized on hydrophobic supports

    Article • Journal of Molecular Catalysis B Enzymatic, October 2013, Elsevier

    Dr ROBERTO FERNANDEZ-LAFUENTE

  32. Optimized butyl butyrate synthesis catalyzed byThermomyces lanuginosuslipase

    Article • Biotechnology Progress, September 2013, Wiley

    Dr ROBERTO FERNANDEZ-LAFUENTE

  33. Biotechnological prospects of the lipase from Mucor javanicus

    Article • Journal of Molecular Catalysis B Enzymatic, September 2013, Elsevier

    Dr ROBERTO FERNANDEZ-LAFUENTE

  34. Ultrasound-assisted butyl acetate synthesis catalyzed by Novozym 435: Enhanced activity and operational stability

    Article • Ultrasonics Sonochemistry, September 2013, Elsevier

    Dr ROBERTO FERNANDEZ-LAFUENTE

  35. Heterofunctional Supports in Enzyme Immobilization: From Traditional Immobilization Protocols to Opportunities in Tuning Enzyme Properties

    Article • Biomacromolecules, August 2013, American Chemical Society (ACS)

    Dr ROBERTO FERNANDEZ-LAFUENTE

  36. Characterization of the catalytic properties of lipases from plant seeds for the production of concentrated fatty acids from different vegetable oils

    Article • Industrial Crops and Products, August 2013, Elsevier

    Dr ROBERTO FERNANDEZ-LAFUENTE

  37. Optimization of the immobilization of sweet potato amylase using glutaraldehyde-agarose support. Characterization of the immobilized enzyme

    Article • Process Biochemistry, July 2013, Elsevier

    Dr ROBERTO FERNANDEZ-LAFUENTE

  38. Stabilization of the hexameric glutamate dehydrogenase from Escherichia coli by cations and polyethyleneimine

    Article • Enzyme and Microbial Technology, April 2013, Elsevier

    Dr ROBERTO FERNANDEZ-LAFUENTE

  39. Improved production of butyl butyrate with lipase from Thermomyces lanuginosus immobilized on styrene–divinylbenzene beads

    Article • Bioresource Technology, April 2013, Elsevier

    Dr ROBERTO FERNANDEZ-LAFUENTE

  40. Solid-phase modification with succinic polyethyleneglycol of aminated lipase B from Candida antarctica: Effect of the immobilization protocol on enzyme catalytic properties

    Article • Journal of Molecular Catalysis B Enzymatic, March 2013, Elsevier

    Dr ROBERTO FERNANDEZ-LAFUENTE

  41. Modifying enzyme activity and selectivity by immobilization

    Article • Chemical Society Reviews, January 2013, Royal Society of Chemistry

    Dr ROBERTO FERNANDEZ-LAFUENTE

  42. Fast immunosensing technique to detect Legionella pneumophila in different natural and anthropogenic environments: comparative and collaborative trials

    Article • BMC Microbiology, January 2013, Springer Science + Business Media

    Dr ROBERTO FERNANDEZ-LAFUENTE

  43. Chemical amination of lipase B from Candida antarctica is an efficient solution for the preparation of crosslinked enzyme aggregates

    Article • Process Biochemistry, December 2012, Elsevier

    Dr ROBERTO FERNANDEZ-LAFUENTE

  44. Hydrogen Peroxide in Biocatalysis. A Dangerous Liaison

    Article • Current Organic Chemistry, November 2012, Bentham Science Publishers

    Dr ROBERTO FERNANDEZ-LAFUENTE

  45. Effect of immobilization protocol on optimal conditions of ethyl butyrate synthesis catalyzed by lipase B fromCandida antarctica

    Article • Journal of Chemical Technology & Biotechnology, October 2012, Wiley

    Dr ROBERTO FERNANDEZ-LAFUENTE

  46. Versatility of glutaraldehyde to immobilize lipases: Effect of the immobilization protocol on the properties of lipase B from Candida antarctica

    Article • Process Biochemistry, August 2012, Elsevier

    Dr ROBERTO FERNANDEZ-LAFUENTE

  47. Optimized preparation of CALB-CLEAs by response surface methodology: The necessity to employ a feeder to have an effective crosslinking

    Article • Journal of Molecular Catalysis B Enzymatic, August 2012, Elsevier

    Dr ROBERTO FERNANDEZ-LAFUENTE

  48. The slow-down of the CALB immobilization rate permits to control the inter and intra molecular modification produced by glutaraldehyde

    Article • Process Biochemistry, May 2012, Elsevier

    Dr ROBERTO FERNANDEZ-LAFUENTE

  49. Modulation of the properties of immobilized CALB by chemical modification with 2,3,4-trinitrobenzenesulfonate or ethylendiamine. Advantages of using adsorbed lipases on hydrophobic supports

    Article • Process Biochemistry, May 2012, Elsevier

    Dr ROBERTO FERNANDEZ-LAFUENTE

  50. Effect of solid-phase chemical modification on the features of the lipase from Thermomyces lanuginosus

    Article • Process Biochemistry, March 2012, Elsevier

    Dr ROBERTO FERNANDEZ-LAFUENTE

  51. Optimization of pineapple flavour synthesis by esterification catalysed by immobilized lipase from Rhizomucor miehei

    Article • Flavour and Fragrance Journal, February 2012, Wiley

    Dr ROBERTO FERNANDEZ-LAFUENTE

  52. Immobilization of lipase B from Candida antarctica on porous styrene-divinylbenzene beads improves butyl acetate synthesis

    Article • Biotechnology Progress, January 2012, Wiley

    Dr ROBERTO FERNANDEZ-LAFUENTE

  53. Rapid and high yields of synthesis of butyl acetate catalyzed by Novozym 435: Reaction optimization by response surface methodology

    Article • Process Biochemistry, December 2011, Elsevier

    Dr ROBERTO FERNANDEZ-LAFUENTE

  54. Potential of Different Enzyme Immobilization Strategies to Improve Enzyme Performance

    Article • Advanced Synthesis & Catalysis, November 2011, Wiley

    Dr ROBERTO FERNANDEZ-LAFUENTE

  55. Enhancing the functional properties of thermophilic enzymes by chemical modification and immobilization

    Article • Enzyme and Microbial Technology, September 2011, Elsevier

    Dr ROBERTO FERNANDEZ-LAFUENTE

  56. Effect of the immobilization protocol on the properties of lipase B from Candida antarctica in organic media: Enantiospecifc production of atenolol acetate

    Article • Journal of Molecular Catalysis B Enzymatic, September 2011, Elsevier

    Dr ROBERTO FERNANDEZ-LAFUENTE

  57. Coupling Chemical Modification and Immobilization to Improve the Catalytic Performance of Enzymes

    Article • Advanced Synthesis & Catalysis, July 2011, Wiley

    Dr ROBERTO FERNANDEZ-LAFUENTE

  58. ChemInform Abstract: New Trends in the Recycling of NAD(P)H for the Design of Sustainable Asymmetric Reductions Catalyzed by Dehydrogenases

    Article • ChemInform, June 2011, Wiley

    Dr ROBERTO FERNANDEZ-LAFUENTE

  59. Simple and efficient immobilization of lipase B from Candida antarctica on porous styrene–divinylbenzene beads

    Article • Enzyme and Microbial Technology, June 2011, Elsevier

    Dr ROBERTO FERNANDEZ-LAFUENTE

  60. Hydrolysis of triacetin catalyzed by immobilized lipases: Effect of the immobilization protocol and experimental conditions on diacetin yield

    Article • Enzyme and Microbial Technology, May 2011, Elsevier

    Dr ROBERTO FERNANDEZ-LAFUENTE

  61. Purification, immobilization, and characterization of a specific lipase from Staphylococcus warneri EX17 by enzyme fractionating via adsorption on different hydrophobic supports

    Article • Biotechnology Progress, April 2011, Wiley

    Dr ROBERTO FERNANDEZ-LAFUENTE

  62. Lipase B from Candida antarctica immobilized on octadecyl Sepabeads: A very stable biocatalyst in the presence of hydrogen peroxide

    Article • Process Biochemistry, April 2011, Elsevier

    Dr ROBERTO FERNANDEZ-LAFUENTE

  63. Control of protein immobilization: Coupling immobilization and site-directed mutagenesis to improve biocatalyst or biosensor performance

    Article • Enzyme and Microbial Technology, February 2011, Elsevier

    Dr ROBERTO FERNANDEZ-LAFUENTE

  64. Use of Enzymes in the Production of Semi-Synthetic Penicillins and Cephalosporins: Drawbacks and Perspectives

    Article • Current Medicinal Chemistry, November 2010, Bentham Science Publishers

    Dr ROBERTO FERNANDEZ-LAFUENTE

  65. Lipase from Rhizomucor miehei as a biocatalyst in fats and oils modification

    Article • Journal of Molecular Catalysis B Enzymatic, September 2010, Elsevier

    Dr ROBERTO FERNANDEZ-LAFUENTE

  66. Two step ethanolysis: A simple and efficient way to improve the enzymatic biodiesel synthesis catalyzed by an immobilized–stabilized lipase from Thermomyces lanuginosus

    Article • Process Biochemistry, August 2010, Elsevier

    Dr ROBERTO FERNANDEZ-LAFUENTE

  67. ChemInform Abstract: Regioselective Hydrolysis of Peracetylated α-D-Glucopyranose Catalyzed by Immobilized Lipases in Aqueous Medium. A Facile Preparation of Useful Intermediates for Oligosaccharide Synthesis.

    Article • ChemInform, June 2010, Wiley

    Dr ROBERTO FERNANDEZ-LAFUENTE

  68. Lipase from Rhizomucor miehei as an industrial biocatalyst in chemical process

    Article • Journal of Molecular Catalysis B Enzymatic, June 2010, Elsevier

    Dr ROBERTO FERNANDEZ-LAFUENTE

  69. New Trends in the Recycling of NAD(P)H for the Design of Sustainable Asymmetric Reductions Catalyzed by Dehydrogenases

    Article • Current Organic Chemistry, June 2010, Bentham Science Publishers

    Dr ROBERTO FERNANDEZ-LAFUENTE

  70. ChemInform Abstract: Modulation of Penicillin Acylase Properties via Immobilization Techniques: One-Pot Chemoenzymatic Synthesis of Cephamandole from Cephalosporin C.

    Article • ChemInform, May 2010, Wiley

    Dr ROBERTO FERNANDEZ-LAFUENTE

  71. Lipase from Thermomyces lanuginosus: Uses and prospects as an industrial biocatalyst

    Article • Journal of Molecular Catalysis B Enzymatic, March 2010, Elsevier

    Dr ROBERTO FERNANDEZ-LAFUENTE

  72. Stabilization of multimeric enzymes: Strategies to prevent subunit dissociation

    Article • Enzyme and Microbial Technology, December 2009, Elsevier

    Dr ROBERTO FERNANDEZ-LAFUENTE

  73. Improved reactivation of immobilized-stabilized lipase from Thermomyces lanuginosus by its coating with highly hydrophilic polymers

    Article • Journal of Biotechnology, October 2009, Elsevier

    Dr ROBERTO FERNANDEZ-LAFUENTE

  74. Immobilization–stabilization of the lipase from Thermomyces lanuginosus: Critical role of chemical amination

    Article • Process Biochemistry, September 2009, Elsevier

    Dr ROBERTO FERNANDEZ-LAFUENTE

  75. Purification, immobilization and stabilization of a highly enantioselective alcohol dehydrogenase from Thermus thermophilus HB27 cloned in E. coli

    Article • Process Biochemistry, September 2009, Elsevier

    Dr ROBERTO FERNANDEZ-LAFUENTE

  76. The co-operative effect of physical and covalent protein adsorption on heterofunctional supports

    Article • Process Biochemistry, July 2009, Elsevier

    Dr ROBERTO FERNANDEZ-LAFUENTE

  77. Simple strategy of reactivation of a partially inactivated penicillin g acylase biocatalyst in organic solvent and its impact on the synthesis of β-lactam antibiotics

    Article • Biotechnology and Bioengineering, June 2009, Wiley

    Dr ROBERTO FERNANDEZ-LAFUENTE

  78. Positive effects of the multipoint covalent immobilization in the reactivation of partially inactivated derivatives of lipase from Thermomyces lanuginosus

    Article • Enzyme and Microbial Technology, June 2009, Elsevier

    Dr ROBERTO FERNANDEZ-LAFUENTE

  79. Reactivation of covalently immobilized lipase from Thermomyces lanuginosus

    Article • Process Biochemistry, June 2009, Elsevier

    Dr ROBERTO FERNANDEZ-LAFUENTE

  80. Different derivatives of a lipase display different regioselectivity in the monohydrolysis of per-O-acetylated 1-O-substituted-β-galactopyranosides

    Article • Journal of Molecular Catalysis B Enzymatic, June 2009, Elsevier

    Dr ROBERTO FERNANDEZ-LAFUENTE

  81. Purification and stabilization of a glutamate dehygrogenase from Thermus thermophilus via oriented multisubunit plus multipoint covalent immobilization

    Article • Journal of Molecular Catalysis B Enzymatic, June 2009, Elsevier

    Dr ROBERTO FERNANDEZ-LAFUENTE

  82. Novozym 435 displays very different selectivity compared to lipase from Candida antarctica B adsorbed on other hydrophobic supports

    Article • Journal of Molecular Catalysis B Enzymatic, May 2009, Elsevier

    Dr ROBERTO FERNANDEZ-LAFUENTE

  83. Coating of Soluble and Immobilized Enzymes with Ionic Polymers: Full Stabilization of the Quaternary Structure of Multimeric Enzymes

    Article • Biomacromolecules, April 2009, American Chemical Society (ACS)

    Dr ROBERTO FERNANDEZ-LAFUENTE

  84. The adsorption of multimeric enzymes on very lowly activated supports involves more enzyme subunits: Stabilization of a glutamate dehydrogenase from Thermus thermophilus by immobilization on heterofunctional supports

    Article • Enzyme and Microbial Technology, March 2009, Elsevier

    Dr ROBERTO FERNANDEZ-LAFUENTE

  85. Immobilization of antibodies through the surface regions having the highest density in lysine groups on finally inert support surfaces

    Article • Process Biochemistry, March 2009, Elsevier

    Dr ROBERTO FERNANDEZ-LAFUENTE

  86. Chemo-biocatalytic regioselective one-pot synthesis of different deprotected monosaccharides

    Article • Catalysis Today, February 2009, Elsevier

    Dr ROBERTO FERNANDEZ-LAFUENTE

  87. Enhancement of Novozym-435 catalytic properties by physical or chemical modification

    Article • Process Biochemistry, February 2009, Elsevier

    Dr ROBERTO FERNANDEZ-LAFUENTE

  88. Activation of Bacterial Thermoalkalophilic Lipases Is Spurred by Dramatic Structural Rearrangements

    Article • Journal of Biological Chemistry, December 2008, American Society for Biochemistry & Molecular Biology (ASBMB)

    Dr ROBERTO FERNANDEZ-LAFUENTE

  89. Asymmetric hydrolysis of dimethyl 3-phenylglutarate catalyzed by Lecitase Ultra®

    Article • Enzyme and Microbial Technology, December 2008, Elsevier

    Dr ROBERTO FERNANDEZ-LAFUENTE

  90. Separation and Immobilization of Lipase from Penicillium simplicissimum by Selective Adsorption on Hydrophobic Supports

    Article • Applied Biochemistry and Biotechnology, November 2008, Springer Science + Business Media

    Dr ROBERTO FERNANDEZ-LAFUENTE

  91. Regioselective monohydrolysis of per-O-acetylated-1-substituted-β-glucopyranosides catalyzed by immobilized lipases

    Article • Tetrahedron, November 2008, Elsevier

    Dr ROBERTO FERNANDEZ-LAFUENTE

  92. Preparation of an immobilized–stabilized catalase derivative from Aspergillus niger having its multimeric structure stabilized: The effect of Zn2+ on enzyme stability

    Article • Journal of Molecular Catalysis B Enzymatic, November 2008, Elsevier

    Dr ROBERTO FERNANDEZ-LAFUENTE

  93. Crystallization and preliminary X-ray diffraction studies of the BTL2 lipase from the extremophilic microorganismBacillus thermocatenulatus

    Article • Acta Crystallographica Section F Structural Biology and Crystallization Communications, October 2008, International Union of Crystallography

    Dr ROBERTO FERNANDEZ-LAFUENTE

  94. Interfacially activated lipases against hydrophobic supports: Effect of the support nature on the biocatalytic properties

    Article • Process Biochemistry, October 2008, Elsevier

    Dr ROBERTO FERNANDEZ-LAFUENTE

  95. Reversible immobilization of a hexameric α-galactosidase from Thermus sp. strain T2 on polymeric ionic exchangers

    Article • Process Biochemistry, October 2008, Elsevier

    Dr ROBERTO FERNANDEZ-LAFUENTE

  96. Solid-Phase Chemical Amination of a Lipase from Bacillus thermocatenulatus To Improve Its Stabilization via Covalent Immobilization on Highly Activated Glyoxyl-Agarose

    Article • Biomacromolecules, September 2008, American Chemical Society (ACS)

    Dr ROBERTO FERNANDEZ-LAFUENTE

  97. New Cationic Exchanger Support for Reversible Immobilization of Proteins

    Article • Biotechnology Progress, September 2008, Wiley

    Dr ROBERTO FERNANDEZ-LAFUENTE

  98. Thermodynamically Controlled Synthesis of Amide Bonds Catalyzed by Highly Organic Solvent-Resistant Penicillin Acylase Derivatives

    Article • Biotechnology Progress, September 2008, Wiley

    Dr ROBERTO FERNANDEZ-LAFUENTE

  99. Reversible Immobilization of Glutaryl Acylase on Sepabeads Coated with Polyethyleneimine

    Article • Biotechnology Progress, September 2008, Wiley

    Dr ROBERTO FERNANDEZ-LAFUENTE

  100. Purification, Immobilization, and Stabilization of a Lipase from Bacillus thermocatenulatus by Interfacial Adsorption on Hydrophobic Supports

    Article • Biotechnology Progress, September 2008, Wiley

    Dr ROBERTO FERNANDEZ-LAFUENTE

  101. Stabilization of a Multimeric β-Galactosidase from Thermus sp. Strain T2 by Immobilization on Novel Heterofunctional Epoxy Supports Plus Aldehyde-Dextran Cross-Linking

    Article • Biotechnology Progress, September 2008, Wiley

    Dr ROBERTO FERNANDEZ-LAFUENTE

  102. Preparation of linear oligosaccharides by a simple monoprotective chemo-enzymatic approach

    Article • Tetrahedron, September 2008, Elsevier

    Dr ROBERTO FERNANDEZ-LAFUENTE

  103. Stabilization of an Amylase fromNeurospora crassaby Immobilization on Highly Activated Supports

    Article • Food Biotechnology, August 2008, Taylor & Francis

    Dr ROBERTO FERNANDEZ-LAFUENTE

  104. Immobilization–stabilization of a new recombinant glutamate dehydrogenase from Thermus thermophilus

    Article • Applied Microbiology and Biotechnology, August 2008, Springer Science + Business Media

    Dr ROBERTO FERNANDEZ-LAFUENTE

  105. Covalent Immobilization of Antibodies on Finally Inert Support Surfaces through their Surface Regions Having the Highest Densities in Carboxyl Groups

    Article • Biomacromolecules, August 2008, American Chemical Society (ACS)

    Dr ROBERTO FERNANDEZ-LAFUENTE

  106. A chemo-biocatalytic approach in the synthesis of β-O-naphtylmethyl-N-peracetylated lactosamine

    Article • Journal of Molecular Catalysis B Enzymatic, June 2008, Elsevier

    Dr ROBERTO FERNANDEZ-LAFUENTE

  107. Lecitase® ultra as regioselective biocatalyst in the hydrolysis of fully protected carbohydrates

    Article • Journal of Molecular Catalysis B Enzymatic, April 2008, Elsevier

    Dr ROBERTO FERNANDEZ-LAFUENTE

  108. Hybrid coatings as transducers in optical biosensors

    Article • Journal of Coatings Technology and Research, March 2008, Springer Science + Business Media

    Dr ROBERTO FERNANDEZ-LAFUENTE

  109. Oriented Covalent Immobilization of Antibodies on Physically Inert and Hydrophilic Support Surfaces through Their Glycosidic Chains

    Article • Biomacromolecules, February 2008, American Chemical Society (ACS)

    Dr ROBERTO FERNANDEZ-LAFUENTE

  110. Immobilization–stabilization of an α-galactosidase from Thermus sp. strain T2 by covalent immobilization on highly activated supports: Selection of the optimal immobilization strategy

    Article • Enzyme and Microbial Technology, February 2008, Elsevier

    Dr ROBERTO FERNANDEZ-LAFUENTE

  111. Stabilization of the quaternary structure of a hexameric alpha-galactosidase from Thermus sp. T2 by immobilization and post-immobilization techniques

    Article • Process Biochemistry, February 2008, Elsevier

    Dr ROBERTO FERNANDEZ-LAFUENTE

  112. Evaluation of Different Glutaryl Acylase Mutants to Improve the Hydolysis of Cephalosporin C in the Absence of Hydrogen Peroxide

    Article • Advanced Synthesis & Catalysis, January 2008, Wiley

    Dr ROBERTO FERNANDEZ-LAFUENTE

  113. Heterogeneous Enzyme Kinetics

    Article • January 2008, Springer Science + Business Media

    Dr ROBERTO FERNANDEZ-LAFUENTE

  114. Immobilization of the acylase from Escherichia coli on glyoxyl-agarose gives efficient catalyst for the synthesis of cephalosporins

    Article • Enzyme and Microbial Technology, January 2008, Elsevier

    Dr ROBERTO FERNANDEZ-LAFUENTE, Professor Andres R. Alcantara

  115. Polyethyleneimine (PEI) functionalized ceramic monoliths as enzyme carriers: Preparation and performance

    Article • Journal of Molecular Catalysis B Enzymatic, January 2008, Elsevier

    Dr ROBERTO FERNANDEZ-LAFUENTE

  116. Advances in the design of new epoxy supports for enzyme immobilization–stabilization

    Article • Biochemical Society Transactions, December 2007, Portland Press Ltd.

    Dr ROBERTO FERNANDEZ-LAFUENTE

  117. Influence of mass transfer limitations on the enzymatic synthesis of β-lactam antibiotics catalyzed by penicillin G acylase immobilized on glioxil-agarose

    Article • Bioprocess and Biosystems Engineering, November 2007, Springer Science + Business Media

    Dr ROBERTO FERNANDEZ-LAFUENTE

  118. Immobilization of Yarrowia lipolytica Lipase—a Comparison of Stability of Physical Adsorption and Covalent Attachment Techniques

    Article • Applied Biochemistry and Biotechnology, November 2007, Springer Science + Business Media

    Dr ROBERTO FERNANDEZ-LAFUENTE

  119. Screening of lipases for regioselective hydrolysis of peracetylated β-monosaccharides

    Article • Journal of Molecular Catalysis B Enzymatic, November 2007, Elsevier

    Dr ROBERTO FERNANDEZ-LAFUENTE

  120. Specificity enhancement towards hydrophobic substrates by immobilization of lipases by interfacial activation on hydrophobic supports

    Article • Enzyme and Microbial Technology, October 2007, Elsevier

    Dr ROBERTO FERNANDEZ-LAFUENTE

  121. Enzymatic synthesis of cephalosporins. The immobilized acylase from Arthrobacter viscosus: A new useful biocatalyst

    Article • Applied Microbiology and Biotechnology, September 2007, Springer Science + Business Media

    Dr ROBERTO FERNANDEZ-LAFUENTE

  122. Optical fibre biosensors using enzymatic transducers to monitor glucose

    Article • Measurement Science and Technology, September 2007, Institute of Physics Publishing

    Dr ROBERTO FERNANDEZ-LAFUENTE

  123. Enhancement of the activity of an industrial β-galactosidase from Kluyveromies lactis by metal cations: Kinetic modelling

    Article • Journal of Biotechnology, September 2007, Elsevier

    Dr ROBERTO FERNANDEZ-LAFUENTE

  124. Regioselective Hydrolysis of Different Peracetylated β-Monosaccharides by Immobilized Lipases from Different Sources. Key Role of The Immobilization

    Article • Advanced Synthesis & Catalysis, August 2007, Wiley

    Dr ROBERTO FERNANDEZ-LAFUENTE

  125. Partial Purification and Immobilization/Stabilization on Highly Activated Glyoxyl-agarose Supports of Different Proteases from Flavourzyme

    Article • Journal of Agricultural and Food Chemistry, August 2007, American Chemical Society (ACS)

    Dr ROBERTO FERNANDEZ-LAFUENTE

  126. Effect of the immobilization protocol in the activity, stability, and enantioslectivity of Lecitase® Ultra

    Article • Journal of Molecular Catalysis B Enzymatic, July 2007, Elsevier

    Dr ROBERTO FERNANDEZ-LAFUENTE

  127. Modulation of Immobilized Lipase Enantioselectivityvia Chemical Amination

    Article • Advanced Synthesis & Catalysis, May 2007, Wiley

    Dr ROBERTO FERNANDEZ-LAFUENTE

  128. Improvement of enzyme activity, stability and selectivity via immobilization techniques

    Article • Enzyme and Microbial Technology, May 2007, Elsevier

    Dr ROBERTO FERNANDEZ-LAFUENTE

  129. Immobilization of enzymes on heterofunctional epoxy supports

    Article • Nature Protocols, April 2007, Springer Science + Business Media

    Dr ROBERTO FERNANDEZ-LAFUENTE

  130. Solid phase proteomics: Dramatic reinforcement of very weak protein–protein interactions

    Article • Journal of Chromatography B, April 2007, Elsevier

    Dr ROBERTO FERNANDEZ-LAFUENTE

  131. Partial and enantioselective hydrolysis of diethyl phenylmalonate by immobilized preparations of lipase from Thermomyces lanuginose

    Article • Enzyme and Microbial Technology, April 2007, Elsevier

    Dr ROBERTO FERNANDEZ-LAFUENTE

  132. Asymmetric hydrolysis of dimethyl phenylmalonate by immobilized penicillin G acylase from E. coli

    Article • Enzyme and Microbial Technology, April 2007, Elsevier

    Dr ROBERTO FERNANDEZ-LAFUENTE

  133. Effect of the support and experimental conditions in the intensity of the multipoint covalent attachment of proteins on glyoxyl-agarose supports: Correlation between enzyme–support linkages and thermal stability

    Article • Enzyme and Microbial Technology, April 2007, Elsevier

    Dr ROBERTO FERNANDEZ-LAFUENTE

  134. Production of a Thermoresistant Alpha-galactosidase fromThermussp. Strain T2 for Food Processing

    Article • Food Biotechnology, March 2007, Taylor & Francis

    Dr ROBERTO FERNANDEZ-LAFUENTE

  135. Evaluation of different immobilization strategies to prepare an industrial biocatalyst of formate dehydrogenase from Candida boidinii

    Article • Enzyme and Microbial Technology, March 2007, Elsevier

    Dr ROBERTO FERNANDEZ-LAFUENTE

  136. Glutaraldehyde modification of lipases adsorbed on aminated supports: A simple way to improve their behaviour as enantioselective biocatalyst

    Article • Enzyme and Microbial Technology, March 2007, Elsevier

    Dr ROBERTO FERNANDEZ-LAFUENTE

  137. Improved Stabilization of Genetically Modified Penicillin G Acylase in the Presence of Organic Cosolvents by Co- Immobilization of the Enzyme with Polyethyleneimine

    Article • Advanced Synthesis & Catalysis, February 2007, Wiley

    Dr ROBERTO FERNANDEZ-LAFUENTE

  138. Mixed Ion Exchange Supports as Useful Ion Exchangers for Protein Purification:  Purification of Penicillin G Acylase from Escherichia coli

    Article • Biomacromolecules, February 2007, American Chemical Society (ACS)

    Dr ROBERTO FERNANDEZ-LAFUENTE

  139. Stabilization of different alcohol oxidases via immobilization and post immobilization techniques

    Article • Enzyme and Microbial Technology, January 2007, Elsevier

    Dr ROBERTO FERNANDEZ-LAFUENTE

  140. Selective adsorption of large proteins on highly activated IMAC supports in the presence of high imidazole concentrations: Purification, reversible immobilization and stabilization of thermophilic α- and β-galactosidases

    Article • Enzyme and Microbial Technology, January 2007, Elsevier

    Dr ROBERTO FERNANDEZ-LAFUENTE

  141. Modulation of the catalytic properties of multimeric β-galactosidase from E. coli by using different immobilization protocols

    Article • Enzyme and Microbial Technology, January 2007, Elsevier

    Dr ROBERTO FERNANDEZ-LAFUENTE

  142. Improved catalytic properties of immobilized lipases by the presence of very low concentrations of detergents in the reaction medium

    Article • Biotechnology and Bioengineering, January 2007, Wiley

    Dr ROBERTO FERNANDEZ-LAFUENTE

  143. Immobilization of Yarrowia lipolytica Lipase—A Comparison of Stability of Physical Adsorption and Covalent Attachment Techniques

    Article • January 2007, Springer Science + Business Media

    Dr ROBERTO FERNANDEZ-LAFUENTE

  144. Genetic Modification of the Penicillin G Acylase Surface To Improve Its Reversible Immobilization on Ionic Exchangers

    Article • Applied and Environmental Microbiology, November 2006, ASM Journals

    Dr ROBERTO FERNANDEZ-LAFUENTE

  145. Chemical Modification of Protein Surfaces To Improve Their Reversible Enzyme Immobilization on Ionic Exchangers

    Article • Biomacromolecules, November 2006, American Chemical Society (ACS)

    Dr ROBERTO FERNANDEZ-LAFUENTE

  146. Immobilization and Stabilization of a Cyclodextrin Glycosyltransferase by Covalent Attachment on Highly Activated Glyoxyl-Agarose Supports

    Article • Biotechnology Progress, August 2006, Wiley

    Dr ROBERTO FERNANDEZ-LAFUENTE

  147. Supports coated with PEI as a new tool in chromatography

    Article • Enzyme and Microbial Technology, August 2006, Elsevier

    Dr ROBERTO FERNANDEZ-LAFUENTE

  148. CLEAs of lipases and poly-ionic polymers: A simple way of preparing stable biocatalysts with improved properties

    Article • Enzyme and Microbial Technology, August 2006, Elsevier

    Dr ROBERTO FERNANDEZ-LAFUENTE

  149. Purification and identification of different lipases contained in PPL commercial extracts: A minor contaminant is the main responsible of most esterasic activity

    Article • Enzyme and Microbial Technology, August 2006, Elsevier

    Dr ROBERTO FERNANDEZ-LAFUENTE

  150. Different mechanisms of protein immobilization on glutaraldehyde activated supports: Effect of support activation and immobilization conditions

    Article • Enzyme and Microbial Technology, August 2006, Elsevier

    Dr ROBERTO FERNANDEZ-LAFUENTE

  151. Purification and very strong reversible immobilization of large proteins on anionic exchangers by controlling the support and the immobilization conditions

    Article • Enzyme and Microbial Technology, August 2006, Elsevier

    Dr ROBERTO FERNANDEZ-LAFUENTE

  152. Improvement of the stability of alcohol dehydrogenase by covalent immobilization on glyoxyl-agarose

    Article • Journal of Biotechnology, August 2006, Elsevier

    Dr ROBERTO FERNANDEZ-LAFUENTE

  153. Glyoxyl agarose: A fully inert and hydrophilic support for immobilization and high stabilization of proteins

    Article • Enzyme and Microbial Technology, June 2006, Elsevier

    Dr ROBERTO FERNANDEZ-LAFUENTE

  154. Effect of lipase–lipase interactions in the activity, stability and specificity of a lipase from Alcaligenes sp.

    Article • Enzyme and Microbial Technology, June 2006, Elsevier

    Dr ROBERTO FERNANDEZ-LAFUENTE

  155. Use of polyvalent cations to improve the adsorption strength between adsorbed enzymes and supports coated with dextran sulfate

    Article • Enzyme and Microbial Technology, June 2006, Elsevier

    Dr ROBERTO FERNANDEZ-LAFUENTE

  156. Improvement of the enantioselectivity of lipase (fraction B) from Candida antarctica via adsorpiton on polyethylenimine-agarose under different experimental conditions

    Article • Enzyme and Microbial Technology, June 2006, Elsevier

    Dr ROBERTO FERNANDEZ-LAFUENTE

  157. Glyoxyl agarose as a new chromatographic matrix

    Article • Enzyme and Microbial Technology, May 2006, Elsevier

    Dr ROBERTO FERNANDEZ-LAFUENTE

  158. Improvement of the functional properties of a thermostable lipase from alcaligenes sp. via strong adsorption on hydrophobic supports

    Article • Enzyme and Microbial Technology, May 2006, Elsevier

    Dr ROBERTO FERNANDEZ-LAFUENTE

  159. Simple Purification of Immunoglobulins from Whey Proteins Concentrate

    Article • Biotechnology Progress, April 2006, Wiley

    Dr ROBERTO FERNANDEZ-LAFUENTE

  160. Adsorption Behavior of Bovine Serum Albumin on Lowly Activated Anionic Exchangers Suggests a New Strategy for Solid-Phase Proteomics

    Article • Biomacromolecules, April 2006, American Chemical Society (ACS)

    Dr ROBERTO FERNANDEZ-LAFUENTE

  161. Stabilization of a Formate Dehydrogenase by Covalent Immobilization on Highly Activated Glyoxyl-Agarose Supports

    Article • Biomacromolecules, March 2006, American Chemical Society (ACS)

    Dr ROBERTO FERNANDEZ-LAFUENTE

  162. Unusual enzymatic resolution of (±)-glycidyl-butyrate for the production of (S)-glycidyl derivatives

    Article • Enzyme and Microbial Technology, February 2006, Elsevier

    Dr ROBERTO FERNANDEZ-LAFUENTE

  163. Detecting minimal traces of DNA using DNA covalently attached to superparamagnetic nanoparticles and direct PCR-ELISA

    Article • Biosensors and Bioelectronics, February 2006, Elsevier

    Dr ROBERTO FERNANDEZ-LAFUENTE

  164. Crosslinked Penicillin Acylase Aggregates for Synthesis of β-Lactam Antibiotics in Organic Medium

    Article • Applied Biochemistry and Biotechnology, January 2006, Springer Science + Business Media

    Dr ROBERTO FERNANDEZ-LAFUENTE

  165. Preparation of a very stable immobilized biocatalyst of glucose oxidase from Aspergillus niger

    Article • Journal of Biotechnology, January 2006, Elsevier

    Dr ROBERTO FERNANDEZ-LAFUENTE

  166. Improved Stabilization of Chemically Aminated Enzymes Via Multipoint Covalent Attachment on Glyoxyl Supports

    Article • January 2006, Springer Science + Business Media

    Dr ROBERTO FERNANDEZ-LAFUENTE

  167. Purification, Immobilization, Hyperactivation, and Stabilization of Lipases by Selective Adsorption on Hydrophobic Supports

    Article • January 2006, Springer Science + Business Media

    Dr ROBERTO FERNANDEZ-LAFUENTE

  168. Immobilization and Stabilization of Proteins by Multipoint Covalent Attachment on Novel Amino-Epoxy-Sepabeads®

    Article • January 2006, Springer Science + Business Media

    Dr ROBERTO FERNANDEZ-LAFUENTE

  169. One-Step Purification, Immobilization, and Stabilization of Poly-Histidine-Tagged Enzymes Using Metal Chelate-Epoxy Supports

    Article • January 2006, Springer Science + Business Media

    Dr ROBERTO FERNANDEZ-LAFUENTE

  170. Immobilization-Stabilization of Enzymes by Multipoint Covalent Attachment on Supports Activated With Epoxy Groups

    Article • January 2006, Springer Science + Business Media

    Dr ROBERTO FERNANDEZ-LAFUENTE

  171. Very Strong But Reversible Immobilization of Enzymes on Supports Coated With Ionic Polymers

    Article • January 2006, Springer Science + Business Media

    Dr ROBERTO FERNANDEZ-LAFUENTE

  172. Stabilization of Multimeric Enzymes Via Immobilization and Further Cross-Linking With Aldehyde-Dextran

    Article • January 2006, Springer Science + Business Media

    Dr ROBERTO FERNANDEZ-LAFUENTE

  173. Preparation of a robust biocatalyst of d-amino acid oxidase on sepabeads supports using the glutaraldehyde crosslinking method

    Article • Enzyme and Microbial Technology, December 2005, Elsevier

    Dr ROBERTO FERNANDEZ-LAFUENTE

  174. Stabilization of enzymes by multipoint attachment via reversible immobilization on phenylboronic activated supports

    Article • Journal of Biotechnology, December 2005, Elsevier

    Dr ROBERTO FERNANDEZ-LAFUENTE

  175. Optimization of the modification of carrier proteins with aminated haptens

    Article • Journal of Immunological Methods, December 2005, Elsevier

    Dr ROBERTO FERNANDEZ-LAFUENTE

  176. One-Pot Conversion of Cephalosporin C to 7-Aminocephalosporanic Acid in the Absence of Hydrogen Peroxide

    Article • Advanced Synthesis & Catalysis, November 2005, Wiley

    Dr ROBERTO FERNANDEZ-LAFUENTE

  177. Purification, stabilization, and concentration of very weak protein-protein complexes: Shifting the association equilibrium via complex selective adsorption on lowly activated supports

    Article • PROTEOMICS, November 2005, Wiley

    Dr ROBERTO FERNANDEZ-LAFUENTE

  178. Conformational Engineering of Lipases via Directed Immobilisation: Improving the Resolution of Chiral Drugs

    Article • Medicinal Chemistry Reviews - Online, October 2005, Bentham Science Publishers

    Dr ROBERTO FERNANDEZ-LAFUENTE

  179. Some special features of glyoxyl supports to immobilize proteins

    Article • Enzyme and Microbial Technology, September 2005, Elsevier

    Dr ROBERTO FERNANDEZ-LAFUENTE

  180. Enzyme stabilization by glutaraldehyde crosslinking of adsorbed proteins on aminated supports

    Article • Journal of Biotechnology, September 2005, Elsevier

    Dr ROBERTO FERNANDEZ-LAFUENTE

  181. Increasing the binding strength of proteins to PEI coated supports by immobilizing at high ionic strength

    Article • Enzyme and Microbial Technology, August 2005, Elsevier

    Dr ROBERTO FERNANDEZ-LAFUENTE

  182. Immobilization and stabilization of glutaryl acylase on aminated sepabeads supports by the glutaraldehyde crosslinking method

    Article • Journal of Molecular Catalysis B Enzymatic, August 2005, Elsevier

    Dr ROBERTO FERNANDEZ-LAFUENTE

  183. Solid-Phase Lipase Engineering: A New Tool in Combinatorial Biocatalysis

    Article • ChemInform, July 2005, Wiley

    Dr ROBERTO FERNANDEZ-LAFUENTE

  184. Co-aggregation of Enzymes and Polyethyleneimine:  A Simple Method To Prepare Stable and Immobilized Derivatives of Glutaryl Acylase

    Article • Biomacromolecules, July 2005, American Chemical Society (ACS)

    Dr ROBERTO FERNANDEZ-LAFUENTE

  185. Recent Advances in the Industrial Enzymatic Synthesis of Semi-Synthetic βLactam Antibiotics

    Article • Medicinal Chemistry Reviews - Online, June 2005, Bentham Science Publishers

    Dr ROBERTO FERNANDEZ-LAFUENTE

  186. Penicillin G acylase catalyzed acylation of 7-ACA in aqueous two-phase systems using kinetically and thermodynamically controlled strategies: improved enzymatic synthesis of 7-[(1-hydroxy-1-phenyl)-acetamido]-3-acetoxymethyl-Δ3-cephem-4-carboxylic acid

    Article • Enzyme and Microbial Technology, April 2005, Elsevier

    Dr ROBERTO FERNANDEZ-LAFUENTE

  187. Aldehyde–dextran–protein conjugates to immobilize amino-haptens: avoiding cross-reactions in the immunodetection

    Article • Enzyme and Microbial Technology, March 2005, Elsevier

    Dr ROBERTO FERNANDEZ-LAFUENTE

  188. Advantages of the Pre-Immobilization of Enzymes on Porous Supports for Their Entrapment in Sol−Gels

    Article • Biomacromolecules, March 2005, American Chemical Society (ACS)

    Dr ROBERTO FERNANDEZ-LAFUENTE

  189. Lipase–lipase interactions as a new tool to immobilize and modulate the lipase properties

    Article • Enzyme and Microbial Technology, March 2005, Elsevier

    Dr ROBERTO FERNANDEZ-LAFUENTE

  190. Improved stabilization of chemically aminated enzymes via multipoint covalent attachment on glyoxyl supports

    Article • Journal of Biotechnology, March 2005, Elsevier

    Dr ROBERTO FERNANDEZ-LAFUENTE

  191. Synthesis of enantiomerically pure glycidol via a fully enantioselective lipase-catalyzed resolution

    Article • Tetrahedron Asymmetry, February 2005, Elsevier

    Dr ROBERTO FERNANDEZ-LAFUENTE

  192. Preparation of inert magnetic nano-particles for the directed immobilization of antibodies

    Article • Biosensors and Bioelectronics, January 2005, Elsevier

    Dr ROBERTO FERNANDEZ-LAFUENTE

  193. Stabilization of enzymes by multipoint immobilization of thiolated proteins on new epoxy-thiol supports

    Article • Biotechnology and Bioengineering, January 2005, Wiley

    Dr ROBERTO FERNANDEZ-LAFUENTE

  194. Preparation of an Industrial Biocatalyst of Penicillin G Acylase on Sepabeads

    Article • January 2005, Springer Science + Business Media

    Dr ROBERTO FERNANDEZ-LAFUENTE

  195. Dextran aldehyde coating of glucose oxidase immobilized on magnetic nanoparticles prevents its inactivation by gas bubbles

    Article • Journal of Molecular Catalysis B Enzymatic, January 2005, Elsevier

    Dr ROBERTO FERNANDEZ-LAFUENTE

  196. Purification of different lipases fromAspergillus niger by using a highly selective adsorption on hydrophobic supports

    Article • Biotechnology and Bioengineering, January 2005, Wiley

    Dr ROBERTO FERNANDEZ-LAFUENTE

  197. Detection and purification of two antibody–antigen complexes via selective adsorption on lowly activated anion exchangers

    Article • Journal of Chromatography A, December 2004, Elsevier

    Dr ROBERTO FERNANDEZ-LAFUENTE

  198. Use of an Antisense RNA Strategy To Investigate the Functional Significance of Mn-Catalase in the Extreme Thermophile Thermus thermophilus

    Article • Journal of Bacteriology, November 2004, ASM Journals

    Dr ROBERTO FERNANDEZ-LAFUENTE

  199. Immobilization and Stabilization of Recombinant Multimeric Uridine and Purine Nucleoside Phosphorylases fromBacillus subtilis

    Article • Biomacromolecules, November 2004, American Chemical Society (ACS)

    Dr ROBERTO FERNANDEZ-LAFUENTE

  200. Selective and mild adsorption of large proteins on lowly activated immobilized metal ion affinity chromatography matrices

    Article • Journal of Chromatography A, November 2004, Elsevier

    Dr ROBERTO FERNANDEZ-LAFUENTE

  201. A Simple Strategy for the Purification of Large Thermophilic Proteins Overexpressed in Mesophilic Microorganisms: Application to Multimeric Enzymes from Thermus sp. Strain T2 Expressed in Escherichia coli

    Article • Biotechnology Progress, October 2004, Wiley

    Dr ROBERTO FERNANDEZ-LAFUENTE

  202. Determination of protein-protein interactions through aldehyde-dextran intermolecular cross-linking

    Article • PROTEOMICS, September 2004, Wiley

    Dr ROBERTO FERNANDEZ-LAFUENTE

  203. Synthesis of 2′-Deoxynucleosides by Transglycosylation with New Immobilized and Stabilized Uridine Phosphorylase and Purine Nucleoside Phosphorylase

    Article • Advanced Synthesis & Catalysis, September 2004, Wiley

    Dr ROBERTO FERNANDEZ-LAFUENTE

  204. Immobilization of Rennet from Mucor m iehei via Its Sugar Chain. Its Use in Milk Coagulation

    Article • Biomacromolecules, September 2004, American Chemical Society (ACS)

    Dr ROBERTO FERNANDEZ-LAFUENTE

  205. Thermus thermophilus as a Cell Factory for the Production of a Thermophilic Mn-Dependent Catalase Which Fails To Be Synthesized in an Active Form in Escherichia coli

    Article • Applied and Environmental Microbiology, July 2004, ASM Journals

    Dr ROBERTO FERNANDEZ-LAFUENTE

  206. Optimization of an industrial biocatalyst of glutaryl acylase: Stabilization of the enzyme by multipoint covalent attachment onto new amino-epoxy Sepabeads

    Article • Journal of Biotechnology, July 2004, Elsevier

    Dr ROBERTO FERNANDEZ-LAFUENTE

  207. Different Properties of the Lipases Contained in Porcine Pancreatic Lipase Extracts as Enantioselective Biocatalysts

    Article • Biotechnology Progress, June 2004, Wiley

    Dr ROBERTO FERNANDEZ-LAFUENTE

  208. Enantioselective Synthesis of Phenylacetamides in the Presence of High Organic Cosolvent Concentrations Catalyzed by Stabilized Penicillin G Acylase. Effect of the Acyl Donor

    Article • Biotechnology Progress, June 2004, Wiley

    Dr ROBERTO FERNANDEZ-LAFUENTE

  209. Use of immobilized lipases for lipase purification via specific lipase–lipase interactions

    Article • Journal of Chromatography A, June 2004, Elsevier

    Dr ROBERTO FERNANDEZ-LAFUENTE

  210. Directed Covalent Immobilization of Aminated DNA Probes on Aminated Plates

    Article • Biomacromolecules, May 2004, American Chemical Society (ACS)

    Dr ROBERTO FERNANDEZ-LAFUENTE

  211. Prevention of interfacial inactivation of enzymes by coating the enzyme surface with dextran-aldehyde

    Article • Journal of Biotechnology, May 2004, Elsevier

    Dr ROBERTO FERNANDEZ-LAFUENTE

  212. Cross-Linked Aggregates of Multimeric Enzymes:  A Simple and Efficient Methodology To Stabilize Their Quaternary Structure

    Article • Biomacromolecules, May 2004, American Chemical Society (ACS)

    Dr ROBERTO FERNANDEZ-LAFUENTE

  213. Co-Aggregation of Penicillin G Acylase and Polyionic Polymers:  An Easy Methodology To Prepare Enzyme Biocatalysts Stable in Organic Media

    Article • Biomacromolecules, May 2004, American Chemical Society (ACS)

    Dr ROBERTO FERNANDEZ-LAFUENTE

  214. Enzymatic resolution of (±)-glycidyl butyrate in aqueous media. Strong modulation of the properties of the lipase from Rhizopus oryzae via immobilization techniques

    Article • Tetrahedron Asymmetry, April 2004, Elsevier

    Dr ROBERTO FERNANDEZ-LAFUENTE

  215. Ion exchange using poorly activated supports, an easy way for purification of large proteins

    Article • Journal of Chromatography A, April 2004, Elsevier

    Dr ROBERTO FERNANDEZ-LAFUENTE

  216. Resolution of paroxetine precursor using different lipases

    Article • Enzyme and Microbial Technology, March 2004, Elsevier

    Dr ROBERTO FERNANDEZ-LAFUENTE

  217. Stabilization of Penicillin G Acylase from Escherichia coli: Site-Directed Mutagenesis of the Protein Surface To Increase Multipoint Covalent Attachment

    Article • Applied and Environmental Microbiology, February 2004, ASM Journals

    Dr ROBERTO FERNANDEZ-LAFUENTE

  218. Improving the Activity of Lipases from Thermophilic Organisms at Mesophilic Temperatures for Biotechnology Applications

    Article • Biomacromolecules, January 2004, American Chemical Society (ACS)

    Dr ROBERTO FERNANDEZ-LAFUENTE

  219. Improving the Industrial Production of 6-APA: Enzymatic Hydrolysis of Penicillin G in the Presence of Organic Solvents

    Article • Biotechnology Progress, December 2003, Wiley

    Dr ROBERTO FERNANDEZ-LAFUENTE

  220. Evaluation of the lipase from Bacillus thermocatenulatus as an enantioselective biocatalyst

    Article • Tetrahedron Asymmetry, November 2003, Elsevier

    Dr ROBERTO FERNANDEZ-LAFUENTE

  221. Novel Bifunctional Epoxy/Thiol-Reactive Support to Immobilize Thiol Containing Proteins by the Epoxy Chemistry

    Article • Biomacromolecules, November 2003, American Chemical Society (ACS)

    Dr ROBERTO FERNANDEZ-LAFUENTE

  222. The immobilization of a thermophilic β-galactosidase on Sepabeads supports decreases product inhibition

    Article • Enzyme and Microbial Technology, August 2003, Elsevier

    Dr ROBERTO FERNANDEZ-LAFUENTE

  223. Design of an immobilized preparation of catalase from Thermus thermophilus to be used in a wide range of conditions.

    Article • Enzyme and Microbial Technology, August 2003, Elsevier

    Dr ROBERTO FERNANDEZ-LAFUENTE

  224. Inhibitory effects in the side reactions occurring during the enzymic synthesis of amoxicillin: p-hydroxyphenylglycine methyl ester and amoxicillin hydrolysis

    Article • Biotechnology and Applied Biochemistry, August 2003, Wiley

    Dr ROBERTO FERNANDEZ-LAFUENTE

  225. Regio-selective deprotection of peracetylated sugars via lipase hydrolysis

    Article • Tetrahedron, July 2003, Elsevier

    Dr ROBERTO FERNANDEZ-LAFUENTE

  226. Preparation of a Stable Biocatalyst of Bovine Liver Catalase Using Immobilization and Postimmobilization Techniques

    Article • Biotechnology Progress, June 2003, Wiley

    Dr ROBERTO FERNANDEZ-LAFUENTE

  227. Use of Physicochemical Tools to Determine the Choice of Optimal Enzyme: Stabilization of -Amino Acid Oxidase

    Article • Biotechnology Progress, June 2003, Wiley

    Dr ROBERTO FERNANDEZ-LAFUENTE

  228. A Novel Heterofunctional Epoxy-Amino Sepabeads for a New Enzyme Immobilization Protocol: Immobilization-Stabilization of β-Galactosidase from Aspergillus oryzae

    Article • Biotechnology Progress, June 2003, Wiley

    Dr ROBERTO FERNANDEZ-LAFUENTE

  229. Covalent immobilisation of manganese peroxidases (MnP) from Phanerochaete chrysosporium and Bjerkandera sp. BOS55

    Article • Enzyme and Microbial Technology, June 2003, Elsevier

    Dr ROBERTO FERNANDEZ-LAFUENTE

  230. Epoxy-Amino Groups:  A New Tool for Improved Immobilization of Proteins by the Epoxy Method

    Article • Biomacromolecules, May 2003, American Chemical Society (ACS)

    Dr ROBERTO FERNANDEZ-LAFUENTE

  231. Hydrolysis of Proteins by Immobilized-Stabilized Alcalase-Glyoxyl Agarose

    Article • Biotechnology Progress, April 2003, Wiley

    Dr ROBERTO FERNANDEZ-LAFUENTE

  232. Design of New Immobilized-Stabilized Carboxypeptidase A Derivative for Production of Aromatic Free Hydrolysates of Proteins

    Article • Biotechnology Progress, April 2003, Wiley

    Dr ROBERTO FERNANDEZ-LAFUENTE

  233. Overproduction of Thermus sp. Strain T2 β-Galactosidase in Escherichia coli and Preparation by Using Tailor-Made Metal Chelate Supports

    Article • Applied and Environmental Microbiology, April 2003, ASM Journals

    Dr ROBERTO FERNANDEZ-LAFUENTE

  234. Reversible immobilization of a thermophilic β-galactosidase via ionic adsorption on PEI-coated Sepabeads

    Article • Enzyme and Microbial Technology, March 2003, Elsevier

    Dr ROBERTO FERNANDEZ-LAFUENTE

  235. Solid-Phase Handling of Hydrophobins:  Immobilized Hydrophobins as a New Tool To Study Lipases

    Article • Biomacromolecules, March 2003, American Chemical Society (ACS)

    Dr ROBERTO FERNANDEZ-LAFUENTE

  236. Modulation of Mucor miehei lipase properties via directed immobilization on different hetero-functional epoxy resins

    Article • Journal of Molecular Catalysis B Enzymatic, February 2003, Elsevier

    Dr ROBERTO FERNANDEZ-LAFUENTE

  237. Resolution of (±)-5-substituted-6-(5-chloropyridin-2-yl)-7-oxo-5,6-dihydropyrrolo[3,4b]pyrazine derivatives-precursors of (S)-(+)-Zopiclone, catalyzed by immobilized Candida antarctica B lipase in aqueous media

    Article • Tetrahedron Asymmetry, February 2003, Elsevier

    Dr ROBERTO FERNANDEZ-LAFUENTE

  238. Enzymatic transformations. Immobilized A. niger epoxide hydrolase as a novel biocatalytic tool for repeated-batch hydrolytic kinetic resolution of epoxidesPart 54. For part 53 see ref. 21.

    Article • Organic & Biomolecular Chemistry, January 2003, Royal Society of Chemistry

    Dr ROBERTO FERNANDEZ-LAFUENTE

  239. One-Step Purification, Covalent Immobilization, and Additional Stabilization of a Thermophilic Poly-His-Tagged β-Galactosidase from Thermus sp. Strain T2 by using Novel Heterofunctional Chelate−Epoxy Sepabeads

    Article • Biomacromolecules, January 2003, American Chemical Society (ACS)

    Dr ROBERTO FERNANDEZ-LAFUENTE

  240. Reversible Immobilization of Invertase on Sepabeads Coated with Polyethyleneimine: Optimization of the Biocatalyst's Stability

    Article • Biotechnology Progress, December 2002, Wiley

    Dr ROBERTO FERNANDEZ-LAFUENTE

  241. Enzymatic production of (3S,4R)-(−)-4-(4′-fluorophenyl)-6-oxo-piperidin-3-carboxylic acid using a commercial preparation from Candida antarctica A: the role of a contaminant esterase

    Article • Tetrahedron Asymmetry, December 2002, Elsevier

    Dr ROBERTO FERNANDEZ-LAFUENTE

  242. Preparation of artificial hyper-hydrophilic micro-environments (polymeric salts) surrounding enzyme molecules

    Article • Journal of Molecular Catalysis B Enzymatic, December 2002, Elsevier

    Dr ROBERTO FERNANDEZ-LAFUENTE

  243. Interfacial adsorption of lipases on very hydrophobic support (octadecyl–Sepabeads): immobilization, hyperactivation and stabilization of the open form of lipases

    Article • Journal of Molecular Catalysis B Enzymatic, December 2002, Elsevier

    Dr ROBERTO FERNANDEZ-LAFUENTE

  244. Modulation of the enantioselectivity of lipases via controlled immobilization and medium engineering: hydrolytic resolution of mandelic acid esters

    Article • Enzyme and Microbial Technology, November 2002, Elsevier

    Dr ROBERTO FERNANDEZ-LAFUENTE

  245. Enzymatic synthesis of amoxicillin:Avoiding limitations of the mechanistic approach for reaction kinetics

    Article • Biotechnology and Bioengineering, October 2002, Wiley

    Dr ROBERTO FERNANDEZ-LAFUENTE

  246. Enzymatic resolution of (±)-trans-4-(4′-fluorophenyl)-6-oxo-piperidin-3-ethyl carboxylate, an intermediate in the synthesis of (−)-Paroxetine

    Article • Tetrahedron Asymmetry, October 2002, Elsevier

    Dr ROBERTO FERNANDEZ-LAFUENTE

  247. Regioselective enzymatic hydrolysis of acetylated pyranoses and pyranosides using immobilised lipases. An easy chemoenzymatic synthesis of α- and β-d-glucopyranose acetates bearing a free secondary C-4 hydroxyl group

    Article • Carbohydrate Research, October 2002, Elsevier

    Dr ROBERTO FERNANDEZ-LAFUENTE, Agatha Bastida

  248. Industrial Synthesis of Semi-Synthetic β-Lactam Antibiotics: Recent Developments in Enzyme Biocatalysis for Improved and MORE Sustainable Processes

    Article • Current Medicinal Chemistry - Anti-Infective Agents, October 2002, Bentham Science Publishers

    Dr ROBERTO FERNANDEZ-LAFUENTE

  249. The role of 6-aminopenicillanic acid on the kinetics of amoxicillin enzymatic synthesis catalyzed by penicillin G acylase immobilized onto glyoxyl-agarose

    Article • Enzyme and Microbial Technology, September 2002, Elsevier

    Dr ROBERTO FERNANDEZ-LAFUENTE

  250. Modulation of the enantioselectivity of Candida antarctica B lipase via conformational engineering. Kinetic resolution of (±)-α-hydroxy-phenylacetic acid derivatives

    Article • Tetrahedron Asymmetry, July 2002, Elsevier

    Dr ROBERTO FERNANDEZ-LAFUENTE

  251. Influence of the enzyme derivative preparation and substrate structure on the enantioselectivity of penicillin G acylase

    Article • Enzyme and Microbial Technology, July 2002, Elsevier

    Dr ROBERTO FERNANDEZ-LAFUENTE

  252. Epoxy Sepabeads: A Novel Epoxy Support for Stabilization of Industrial Enzymes via Very Intense Multipoint Covalent Attachment

    Article • Biotechnology Progress, June 2002, Wiley

    Dr ROBERTO FERNANDEZ-LAFUENTE

  253. Modulation of penicillin acylase properties via immobilization techniques: one-pot chemoenzymatic synthesis of cephamandole from cephalosporin C

    Article • Bioorganic & Medicinal Chemistry Letters, September 2001, Elsevier

    Dr ROBERTO FERNANDEZ-LAFUENTE

  254. Structural and Functional Stabilization of L-Asparaginase via Multisubunit Immobilization onto Highly Activated Supports

    Article • Biotechnology Progress, June 2001, Wiley

    Dr ROBERTO FERNANDEZ-LAFUENTE, Professor Victor Manuel Balcão

  255. Coimmobilization of L-asparaginase and glutamate dehydrogenase onto highly activated supports

    Article • Enzyme and Microbial Technology, May 2001, Elsevier

    Dr ROBERTO FERNANDEZ-LAFUENTE, Professor Victor Manuel Balcão

  256. Affinity chromatography of polyhistidine tagged enzymes

    Article • Journal of Chromatography A, April 2001, Elsevier

    Dr ROBERTO FERNANDEZ-LAFUENTE

  257. Biotransformations Catalyzed by Multimeric Enzymes:  Stabilization of Tetrameric Ampicillin Acylase Permits the Optimization of Ampicillin Synthesis under Dissociation Conditions

    Article • Biomacromolecules, March 2001, American Chemical Society (ACS)

    Dr ROBERTO FERNANDEZ-LAFUENTE

  258. Modulation of lipase properties in macro-aqueous systems by controlled enzyme immobilization: enantioselective hydrolysis of a chiral ester by immobilized Pseudomonas lipase

    Article • Enzyme and Microbial Technology, March 2001, Elsevier

    Dr ROBERTO FERNANDEZ-LAFUENTE, Agatha Bastida

  259. Electrostatic and covalent immobilisation of enzymes on ITQ-6 delaminated zeolitic materials

    Article • Chemical Communications, January 2001, Royal Society of Chemistry

    Dr ROBERTO FERNANDEZ-LAFUENTE

  260. Stabilization of Immobilized Enzymes Against Water-Soluble Organic Cosolvents and Generation of Hyper-Hydrophilic Micro-Environments Surrounding Enzyme Molecules

    Article • Biocatalysis and Biotransformation, January 2001, Taylor & Francis

    Dr ROBERTO FERNANDEZ-LAFUENTE

  261. Stabilization of a tetrameric enzyme (α-amino acid ester hydrolase from Acetobacter turbidans) enables a very improved performance of ampicillin synthesis

    Article • Journal of Molecular Catalysis B Enzymatic, January 2001, Elsevier

    Dr ROBERTO FERNANDEZ-LAFUENTE

  262. Biocatalyst engineering exerts a dramatic effect on selectivity of hydrolysis catalyzed by immobilized lipases in aqueous medium

    Article • Journal of Molecular Catalysis B Enzymatic, January 2001, Elsevier

    Dr ROBERTO FERNANDEZ-LAFUENTE, Agatha Bastida

  263. Enantioselective enzymatic hydrolysis of racemic glycidyl esters by using immobilized porcine pancreas lipase with improved catalytic properties

    Article • Journal of Molecular Catalysis B Enzymatic, January 2001, Elsevier

    Dr ROBERTO FERNANDEZ-LAFUENTE, Professor Andres R. Alcantara

  264. Preparation of new lipases derivatives with high activity–stability in anhydrous media: adsorption on hydrophobic supports plus hydrophilization with polyethylenimine

    Article • Journal of Molecular Catalysis B Enzymatic, January 2001, Elsevier

    Dr ROBERTO FERNANDEZ-LAFUENTE

  265. Multifunctional Epoxy Supports:  A New Tool To Improve the Covalent Immobilization of Proteins. The Promotion of Physical Adsorptions of Proteins on the Supports before Their Covalent Linkage

    Article • Biomacromolecules, December 2000, American Chemical Society (ACS)

    Dr ROBERTO FERNANDEZ-LAFUENTE

  266. Immobilization of functionally unstable catechol-2,3-dioxygenase greatly improves operational stability

    Article • Enzyme and Microbial Technology, May 2000, Elsevier

    Dr ROBERTO FERNANDEZ-LAFUENTE

  267. Reversible enzyme immobilization via a very strong and nondistorting ionic adsorption on support-polyethylenimine composites

    Article • Biotechnology and Bioengineering, April 2000, Wiley

    Dr ROBERTO FERNANDEZ-LAFUENTE

  268. Essential role of the concentration of immobilized ligands in affinity chromatography:

    Article • Journal of Chromatography B Biomedical Sciences and Applications, April 2000, Elsevier

    Dr ROBERTO FERNANDEZ-LAFUENTE

  269. Increase in conformational stability of enzymes immobilized on epoxy-activated supports by favoring additional multipoint covalent attachment☆

    Article • Enzyme and Microbial Technology, April 2000, Elsevier

    Dr ROBERTO FERNANDEZ-LAFUENTE

  270. A Kinetic Study of Synthesis of Amoxicillin Using Penicillin G Acylase Immobilized on Agarose

    Article • Applied Biochemistry and Biotechnology, January 2000, Springer Science + Business Media

    Dr ROBERTO FERNANDEZ-LAFUENTE

  271. Affinity chromatography of plasma proteins (guanidinobenzoatase): use of mimetic matrices and mimetic soluble ligands to prevent the binding of albumin on target affinity matrices

    Article • Journal of Chromatography B Biomedical Sciences and Applications, September 1999, Elsevier

    Dr ROBERTO FERNANDEZ-LAFUENTE

  272. Stabilization of multimeric enzymes via immobilization and post-immobilization techniques

    Article • Journal of Molecular Catalysis B Enzymatic, September 1999, Elsevier

    Dr ROBERTO FERNANDEZ-LAFUENTE

  273. Stabilization of enzymes (d-amino acid oxidase) against hydrogen peroxide via immobilization and post-immobilization techniques

    Article • Journal of Molecular Catalysis B Enzymatic, September 1999, Elsevier

    Dr ROBERTO FERNANDEZ-LAFUENTE

  274. Evaluation of different enzymes as catalysts for the production of β-lactam antibiotics following a kinetically controlled strategy

    Article • Enzyme and Microbial Technology, August 1999, Elsevier

    Dr ROBERTO FERNANDEZ-LAFUENTE

  275. Selective adsorption of poly-His tagged glutaryl acylase on tailor-made metal chelate supports

    Article • Journal of Chromatography A, July 1999, Elsevier

    Dr ROBERTO FERNANDEZ-LAFUENTE

  276. A controlled fed-batch cultivation for the production of new crude lipases from Candida rugosa with improved properties in fine chemistry

    Article • Journal of Biotechnology, April 1999, Elsevier

    Dr ROBERTO FERNANDEZ-LAFUENTE, Dr Antoni Sánchez

  277. Regioselective hydrolysis of peracetylated α-D-glucopyranose catalyzed by immobilized lipases in aqueous medium. A facile preparation of useful intermediates for oligosaccharide synthesis

    Article • Bioorganic & Medicinal Chemistry Letters, February 1999, Elsevier

    Dr ROBERTO FERNANDEZ-LAFUENTE, Agatha Bastida

  278. Facile synthesis of artificial enzyme nano-environments via solid-phase chemistry of immobilized derivatives: Dramatic stabilization of penicillin acylase versus organic solvents

    Article • Enzyme and Microbial Technology, January 1999, Elsevier

    Dr ROBERTO FERNANDEZ-LAFUENTE

  279. Use of dextrans as long and hydrophilic spacer arms to improve the performance of immobilized proteins acting on macromolecules

    Article • Biotechnology and Bioengineering, November 1998, Wiley

    Dr ROBERTO FERNANDEZ-LAFUENTE

  280. Meta-pathway degradation of phenolics by thermophilic Bacilli

    Article • Enzyme and Microbial Technology, November 1998, Elsevier

    Dr ROBERTO FERNANDEZ-LAFUENTE

  281. ‘Interfacial affinity chromatography’ of lipases: separation of different fractions by selective adsorption on supports activated with hydrophobic groups

    Article • Biochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology, November 1998, Elsevier

    Dr ROBERTO FERNANDEZ-LAFUENTE

  282. The presence of methanol exerts a strong and complex modulation of the synthesis of different antibiotics by immobilized penicillin G acylase

    Article • Enzyme and Microbial Technology, October 1998, Elsevier

    Dr ROBERTO FERNANDEZ-LAFUENTE

  283. Use of aqueous two‐phase systems for in situ extraction of water soluble antibiotics during their synthesis by enzymes immobilized on porous supports

    Article • Biotechnology and Bioengineering, July 1998, Wiley

    Dr ROBERTO FERNANDEZ-LAFUENTE

  284. Use of aqueous two-phase systems for in situ extraction of water soluble antibiotics during their synthesis by enzymes immobilized on porous supports

    Article • Biotechnology and Bioengineering, July 1998, Wiley

    Dr ROBERTO FERNANDEZ-LAFUENTE

  285. A criterion for the selection of monophasic solvents for enzymatic synthesis

    Article • Enzyme and Microbial Technology, July 1998, Elsevier

    Dr ROBERTO FERNANDEZ-LAFUENTE

  286. The coimmobilization of d-amino acid oxidase and catalase enables the quantitative transformation of d-amino acids (d-phenylalanine) into α-keto acids (phenylpyruvic acid)

    Article • Enzyme and Microbial Technology, July 1998, Elsevier

    Dr ROBERTO FERNANDEZ-LAFUENTE

  287. A single step purification, immobilization, and hyperactivation of lipases via interfacial adsorption on strongly hydrophobic supports

    Article • Biotechnology and Bioengineering, June 1998, Wiley

    Dr ROBERTO FERNANDEZ-LAFUENTE

  288. A single step purification, immobilization, and hyperactivation of lipases via interfacial adsorption on strongly hydrophobic supports

    Article • Biotechnology and Bioengineering, June 1998, Wiley

    Dr ROBERTO FERNANDEZ-LAFUENTE

  289. Immobilization of lipases by selective adsorption on hydrophobic supports

    Article • Chemistry and Physics of Lipids, June 1998, Elsevier

    Dr ROBERTO FERNANDEZ-LAFUENTE

  290. Modulation of the properties of penicillin G acylase by acyl donor substrates during n-protection of amino compounds

    Article • Enzyme and Microbial Technology, May 1998, Elsevier

    Dr ROBERTO FERNANDEZ-LAFUENTE

  291. Degradation and denaturation of stable enzymes

    Article • January 1998, Elsevier

    Dr ROBERTO FERNANDEZ-LAFUENTE

  292. Stabilization of immobilized enzymes against organic solvents: Complete hydrophylization of enzymes environments by solidphase chemistry with poly-functional macromolecules.

    Article • January 1998, Elsevier

    Dr ROBERTO FERNANDEZ-LAFUENTE

  293. One-Pot Chemoenzymatic Synthesis of 3‘-Functionalized Cephalosporines (Cefazolin) by Three Consecutive Biotransformations in Fully Aqueous Medium

    Article • The Journal of Organic Chemistry, December 1997, American Chemical Society (ACS)

    Dr ROBERTO FERNANDEZ-LAFUENTE

  294. Chemoenzymatic one-pot synthesis of cefazolin from cephalosporin C in fully aqueous medium, involving three consecutive biotransformations catalyzed by D-aminoacid oxidase, glutaryl acylase and penicillin G acylase

    Article • Tetrahedron Letters, June 1997, Elsevier

    Dr ROBERTO FERNANDEZ-LAFUENTE

  295. Reactivation strategies by unfolding/refolding of chymotrypsin derivatives after inactivation by organic solvents

    Article • Biochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology, April 1997, Elsevier

    Dr ROBERTO FERNANDEZ-LAFUENTE, Agatha Bastida

  296. Synthesis of antibiotics (cephaloglycin) catalyzed by penicillin G acylase: Evaluation and optimization of different synthetic approaches

    Article • Enzyme and Microbial Technology, July 1996, Elsevier

    Dr ROBERTO FERNANDEZ-LAFUENTE

  297. Purification and partial characterization of a novel thermophilic carboxylesterase with high mesophilic specific activity

    Article • Enzyme and Microbial Technology, September 1995, Elsevier

    Dr ROBERTO FERNANDEZ-LAFUENTE

  298. The use of stabilised penicillin acylase derivatives improves the design of kinetically controlled synthesis

    Article • Journal of Molecular Catalysis A Chemical, July 1995, Elsevier

    Dr ROBERTO FERNANDEZ-LAFUENTE

  299. Strategies for enzyme stabilization by intramolecular crosslinking with bifunctional reagents

    Article • Enzyme and Microbial Technology, June 1995, Elsevier

    Dr ROBERTO FERNANDEZ-LAFUENTE

  300. Selective oxidation: stabilisation by multipoint attachment of ferredoxin NADP+ reductase, an interesting cofactor recycling enzyme

    Article • Journal of Molecular Catalysis A Chemical, May 1995, Elsevier

    Dr ROBERTO FERNANDEZ-LAFUENTE

  301. Hyperstabilization of a thermophilic esterase by multipoint covalent attachment

    Article • Enzyme and Microbial Technology, April 1995, Elsevier

    Dr ROBERTO FERNANDEZ-LAFUENTE

  302. Resolution of Racemic Mixtures through Stereospecific Kinetically Controlled Synthesis Catalyzed by Penicillin G Acylase Derivatives

    Article • Annals of the New York Academy of Sciences, March 1995, Wiley

    Dr ROBERTO FERNANDEZ-LAFUENTE

  303. Design of Novel Biocatalysts by "Bioimprinting" during Unfolding-Refolding of Fully Dispersed Covalently Immobilized Enzymes

    Article • Annals of the New York Academy of Sciences, March 1995, Wiley

    Dr ROBERTO FERNANDEZ-LAFUENTE

  304. Reducing enzyme conformational flexibility by multi-point covalent immobilisation

    Article • Biotechnology Techniques, January 1995, Springer Science + Business Media

    Dr ROBERTO FERNANDEZ-LAFUENTE

  305. Modification of Enzyme Properties by the use of Inhibitors During Their Stabilisation by Multipoint Covalent Attachment

    Article • Biocatalysis and Biotransformation, January 1995, Taylor & Francis

    Dr ROBERTO FERNANDEZ-LAFUENTE

  306. Industrial design of enzymic processes catalysed by very active immobilized derivatives: utilization of diffusional limitations (gradients of pH) as a profitable tool in enzyme engineering

    Article • Biotechnology and Applied Biochemistry, December 1994, Wiley

    Dr ROBERTO FERNANDEZ-LAFUENTE

  307. Selective Enzymatic Oxidations by using Oxygen as oxidizing agent: Immobilization and Stabilization of FNR, a NADP+ regenerating enzyme

    Article • January 1994, Elsevier

    Dr ROBERTO FERNANDEZ-LAFUENTE

  308. Resolution of racemic mixtures by synthesis reactions catalyzed by immobilized derivatives of the enzyme penicillin G acylase

    Article • Journal of Molecular Catalysis, October 1993, Elsevier

    Dr ROBERTO FERNANDEZ-LAFUENTE

  309. Syntheses of pharmaceutical oligosaccharides catalyzed by immobilized-stabilized derivatives of different β-galactosidases

    Article • Journal of Molecular Catalysis, October 1993, Elsevier

    Dr ROBERTO FERNANDEZ-LAFUENTE, Agatha Bastida

  310. Stabilization of heterodimeric enzyme by multipoint covalent immobilization: Penicillin G acylase from Kluyvera citrophila

    Article • Biotechnology and Bioengineering, August 1993, Wiley

    Dr ROBERTO FERNANDEZ-LAFUENTE

  311. Preparation of activated supports containing low pK amino groups. A new tool for protein immobilization via the carboxyl coupling method

    Article • Enzyme and Microbial Technology, July 1993, Elsevier

    Dr ROBERTO FERNANDEZ-LAFUENTE, Agatha Bastida

  312. Fully Dispersed and Covalently Attached Chymotrypsin Derivatives as Industrial Catalysts in Biphasic Systems.

    Article • Annals of the New York Academy of Sciences, November 1992, Wiley

    Dr ROBERTO FERNANDEZ-LAFUENTE

  313. Fully Dispersed and Covalently Attached Chymotrypsin Derivatives as Industrial Catalysts in Biphasic Systems.

    Article • Annals of the New York Academy of Sciences, November 1992, Wiley

    Dr ROBERTO FERNANDEZ-LAFUENTE, Agatha Bastida

  314. Additional stabilization of penicillin G acylase-agarose derivatives by controlled chemical modification with formaldehyde

    Article • Enzyme and Microbial Technology, June 1992, Elsevier

    Dr ROBERTO FERNANDEZ-LAFUENTE

  315. Penicillin G acylase fromKluyvera citrophila new choice as industrial enzyme

    Article • Biotechnology Letters, April 1992, Springer Science + Business Media

    Dr ROBERTO FERNANDEZ-LAFUENTE

  316. Enzyme reaction engineering: Synthesis of antibiotics catalysed by stabilized penicillin G acylase in the presence of organic cosolvents

    Article • Enzyme and Microbial Technology, November 1991, Elsevier

    Dr ROBERTO FERNANDEZ-LAFUENTE

  317. Stabilizing effect of penicillin G sulfoxide, a competitive inhibitor of penicillin G acylase: Its practical applications

    Article • Enzyme and Microbial Technology, March 1991, Elsevier

    Dr ROBERTO FERNANDEZ-LAFUENTE

  318. Equilibrium controlled synthesis of cephalothin in water-cosolvent systems by stabilized penicillin G acylase

    Article • Applied Biochemistry and Biotechnology, March 1991, Springer Science + Business Media

    Dr ROBERTO FERNANDEZ-LAFUENTE

  319. Immobilization-Stabilization of Penicillin G Acylase.

    Article • Annals of the New York Academy of Sciences, December 1990, Wiley

    Dr ROBERTO FERNANDEZ-LAFUENTE

  320. Immobilization-stabilization of Penicillin G acylase fromEscherichia coli

    Article • Applied Biochemistry and Biotechnology, November 1990, Springer Science + Business Media

    Dr ROBERTO FERNANDEZ-LAFUENTE