All Stories

  1. Structural Study of Enzymes Involved in Porphyrin Metabolism

    Article • Nihon Kessho Gakkaishi, May 2025, The Crystallographic Society of Japan

    Masakazu Sugishima

  2. Heme Regulatory Motif of Heme Oxygenase-2 Is Involved in the Interaction with NADPH–Cytochrome P450 Reductase and Regulates Enzymatic Activity

    Article • International Journal of Molecular Sciences, March 2025, MDPI AG

    Masakazu Sugishima

  3. Crystal structures of hydroxymethylbilane synthase complexed with a substrate analog: a single substrate-binding site for four consecutive condensation steps

    Article • Biochemical Journal, March 2021, Portland Press Ltd.

    Masakazu Sugishima

  4. Conformational Equilibrium of NADPH–Cytochrome P450 Oxidoreductase Is Essential for Heme Oxygenase Reaction

    Article • Antioxidants, July 2020, MDPI AG

    Masakazu Sugishima

  5. Recent Advances in the Understanding of the Reaction Chemistries of the Heme Catabolizing Enzymes HO and BVR Based on High Resolution Protein Structures

    Article • Current Medicinal Chemistry, June 2020, Bentham Science Publishers

    Masakazu Sugishima

  6. Functional diversification of two bilin reductases for light perception and harvesting in unique cyanobacterium Acaryochloris marina MBIC 11017

    Article • FEBS Journal, January 2020, Wiley

    Masakazu Sugishima

  7. Crystal structure of phytochromobilin synthase in complex with biliverdin IXα, a key enzyme in the biosynthesis of phytochrome

    Article • Journal of Biological Chemistry, January 2020, Elsevier

    Masakazu Sugishima

  8. Crystal structure of phytochromobilin synthase in complex with biliverdin IXα, a key enzyme in the biosynthesis of phytochrome

    Article • Journal of Biological Chemistry, December 2019, American Society for Biochemistry & Molecular Biology (ASBMB)

    Masakazu Sugishima

  9. Bilin-metabolizing enzymes: site-specific reductions catalyzed by two different type of enzymes

    Article • Current Opinion in Structural Biology, December 2019, Elsevier

    Masakazu Sugishima

  10. Coupling of Redox and Structural States in Cytochrome P450 Reductase Studied by Molecular Dynamics Simulation

    Article • Scientific Reports, June 2019, Springer Science + Business Media

    Masakazu Sugishima

  11. Crystal structure of a NADPH ‐cytochrome P450 oxidoreductase ( CYPOR ) and heme oxygenase 1 fusion protein implies a conformational change in CYPOR upon NADPH ...

    Article • FEBS Letters, March 2019, Wiley

    Masakazu Sugishima

  12. Crystal Growth of a Bilin Reductase PcyA I86D Mutant–Substrate Complex for Neutron Crystallography

    Article • Crystal Growth & Design, July 2018, American Chemical Society (ACS)

    Masakazu Sugishima

  13. A substrate-bound structure of cyanobacterial biliverdin reductase identifies stacked substrates as critical for activity

    Article • Nature Communications, February 2017, Springer Science + Business Media

    Masakazu Sugishima

  14. Crystal structure of I86D mutated PcyA in complex with biliverdin

    Article • FEBS Letters, September 2016, Wiley

    Masakazu Sugishima

  15. A microfluidic-based protein crystallization method in 10 micrometer-sized crystallization space

    Article • CrystEngComm, January 2016, Royal Society of Chemistry

    Masakazu Sugishima

  16. Insights into the Proton Transfer Mechanism of a Bilin Reductase PcyA Following Neutron Crystallography

    Article • Journal of the American Chemical Society, April 2015, American Chemical Society (ACS)

    Masakazu Sugishima

  17. Distal Regulation of Heme Binding of Heme Oxygenase-1 Mediated by Conformational Fluctuations

    Article • Biochemistry, December 2014, American Chemical Society (ACS)

    Masakazu Sugishima

  18. Backbone assignments of the apo and Zn(II) protoporphyrin IX-bound states of the soluble form of rat heme oxygenase-1

    Article • Biomolecular NMR Assignments, September 2014, Springer Science + Business Media

    Masakazu Sugishima

  19. Structural basis for the electron transfer from an open form of NADPH-cytochrome P450 oxidoreductase to heme oxygenase

    Article • Proceedings of the National Academy of Sciences, February 2014, Proceedings of the National Academy of Sciences

    Masakazu Sugishima

  20. Discrimination between CO and O2 in Heme Oxygenase: Comparison of Static Structures and Dynamic Conformation Changes following CO Photolysis

    Article • Biochemistry, October 2012, American Chemical Society (ACS)

    Masakazu Sugishima

  21. ACCELERATED CELL DEATH 2 suppresses mitochondrial oxidative bursts and modulates cell death in Arabidopsis

    Article • The Plant Journal, November 2011, Wiley

    Masakazu Sugishima

  22. Caveolin-1 Is a Competitive Inhibitor of Heme Oxygenase-1 (HO-1) with Heme: Identification of a Minimum Sequence in Caveolin-1 for Binding to HO-1

    Article • Biochemistry, August 2011, American Chemical Society (ACS)

    Masakazu Sugishima

  23. Expression, purification and preliminary X-ray crystallographic analysis of cyanobacterial biliverdin reductase

    Article • Acta Crystallographica Section F Structural Biology and Crystallization Communications, February 2011, International Union of Crystallography

    Masakazu Sugishima

  24. Reduction of oxaporphyrin ring of CO-bound α-verdoheme complexed with heme oxygenase-1 by NADPH-cytochrome P450 reductase

    Article • Journal of Inorganic Biochemistry, February 2011, Elsevier

    Masakazu Sugishima

  25. Crystal Structures of the Substrate-Bound Forms of Red Chlorophyll Catabolite Reductase: Implications for Site-Specific and Stereospecific Reaction

    Article • Journal of Molecular Biology, October 2010, Elsevier

    Masakazu Sugishima

  26. Protein dynamics of heme-heme oxygenase-1 complex following carbon monoxide dissociation

    Article • Journal of Raman Spectroscopy, September 2010, Wiley

    Masakazu Sugishima

  27. Structural Insights into Vinyl Reduction Regiospecificity of Phycocyanobilin:Ferredoxin Oxidoreductase (PcyA)

    Article • Journal of Biological Chemistry, November 2009, American Society for Biochemistry & Molecular Biology (ASBMB)

    Masakazu Sugishima

  28. Crystal Structure of Red Chlorophyll Catabolite Reductase: Enlargement of the Ferredoxin-Dependent Bilin Reductase Family

    Article • Journal of Molecular Biology, June 2009, Elsevier

    Masakazu Sugishima

  29. Crystal structure of rat haem oxygenase-1 in complex with ferrous verdohaem: presence of a hydrogen-bond network on the distal side

    Article • Biochemical Journal, April 2009, Portland Press Ltd.

    Masakazu Sugishima

  30. Involvement of Metals in Enzymatic and Nonenzymatic Decomposition of C-Terminal α-Hydroxyglycine to Amide: An Implication for the Catalytic Role of Enzyme-Bound Zinc in the Peptidylamidoglycolate Lyase Reaction†

    Article • Biochemistry, February 2009, American Chemical Society (ACS)

    Masakazu Sugishima

  31. Mass spectrometric identification of lysine residues of heme oxygenase-1 that are involved in its interaction with NADPH-cytochrome P450 reductase

    Article • Biochemical and Biophysical Research Communications, March 2008, Elsevier

    Masakazu Sugishima

  32. Electrochemical reduction of ferrous α-verdoheme in complex with heme oxygenase-1

    Article • Journal of Inorganic Biochemistry, October 2007, Elsevier

    Masakazu Sugishima

  33. Alternative cyanide-binding modes to the haem iron in haem oxygenase

    Article • Acta Crystallographica Section F Structural Biology and Crystallization Communications, May 2007, International Union of Crystallography

    Masakazu Sugishima

  34. X-ray Crystallographic and Biochemical Characterization of the Inhibitory Action of an Imidazole−Dioxolane Compound on Heme Oxygenase†,‡

    Article • Biochemistry, February 2007, American Chemical Society (ACS)

    Masakazu Sugishima

  35. Induced-fitting and electrostatic potential change of PcyA upon substrate binding demonstrated by the crystal structure of the substrate-free form

    Article • FEBS Letters, June 2006, Wiley

    Masakazu Sugishima

  36. Crystal structure of phycocyanobilin:ferredoxin oxidoreductase in complex with biliverdin IXα, a key enzyme in the biosynthesis of phycocyanobilin

    Article • Proceedings of the National Academy of Sciences, December 2005, Proceedings of the National Academy of Sciences

    Masakazu Sugishima

  37. Crystal Structure of Dimeric Heme Oxygenase-2 fromSynechocystissp. PCC 6803 in Complex with Heme†,‡

    Article • Biochemistry, March 2005, American Chemical Society (ACS)

    Masakazu Sugishima

  38. Structure of photoactive yellow protein (PYP) E46Q mutant at 1.2 Å resolution suggests how Glu46 controls the spectroscopic and kinetic characteristics of PYP

    Article • Acta Crystallographica Section D Biological Crystallography, November 2004, International Union of Crystallography

    Masakazu Sugishima

  39. Crystal structure of heme oxygenase-1 from cyanobacterium Synechocystis sp. PCC 6803 in complex with heme

    Article • FEBS Journal, November 2004, Wiley

    Masakazu Sugishima

  40. Involvement of NADP(H) in the Interaction between Heme Oxygenase-1 and Cytochrome P450 Reductase

    Article • Journal of Biological Chemistry, October 2004, American Society for Biochemistry & Molecular Biology (ASBMB)

    Masakazu Sugishima

  41. CO-trapping Site in Heme Oxygenase Revealed by Photolysis of its CO-bound Heme Complex: Mechanism of Escaping from Product Inhibition

    Article • Journal of Molecular Biology, July 2004, Elsevier

    Masakazu Sugishima

  42. Hydroxylamine and hydrazine bind directly to the heme iron of the heme–heme oxygenase-1 complex

    Article • Journal of Inorganic Biochemistry, July 2004, Elsevier

    Masakazu Sugishima

  43. Crystal Structure of a Novel Zinc-Binding ATP Sulfurylase fromThermus thermophilusHB8†,‡

    Article • Biochemistry, April 2004, American Chemical Society (ACS)

    Masakazu Sugishima

  44. Crystal Structures of Ferrous and CO-, CN--, and NO-Bound Forms of Rat Heme Oxygenase-1 (HO-1) in Complex with Heme:  Structural Implications for Discrimination between CO and O2in HO-1†,‡

    Article • Biochemistry, August 2003, American Chemical Society (ACS)

    Masakazu Sugishima

  45. Crystal Structure of Rat Heme Oxygenase-1 in Complex with Biliverdin-Iron Chelate

    Article • Journal of Biological Chemistry, June 2003, American Society for Biochemistry & Molecular Biology (ASBMB)

    Masakazu Sugishima

  46. Structure and reaction mechanism of heme oxygenase-1

    Article • International Congress Series, November 2002, Elsevier

    Masakazu Sugishima

  47. Crystal Structure of Rat Heme Oxygenase-1 in Complex with Heme Bound to Azide

    Article • Journal of Biological Chemistry, September 2002, American Society for Biochemistry & Molecular Biology (ASBMB)

    Masakazu Sugishima

  48. Crystal Structure of Rat Apo-Heme Oxygenase-1 (HO-1):  Mechanism of Heme Binding in HO-1 Inferred from Structural Comparison of the Apo and Heme Complex Forms†,‡

    Article • Biochemistry, June 2002, American Chemical Society (ACS)

    Masakazu Sugishima

  49. Crystal structure of rat heme oxygenase-1 in complex with heme

    Article • FEBS Letters, April 2000, Wiley

    Masakazu Sugishima