All Stories

  1. Engineering rhamnose-inducible T7 expression systems in Bacillus subtilis utilizing the rhaEW promoter and identifying critical residues in RhaR regulating this promoter

    Article • Bioscience Biotechnology and Biochemistry, May 2025, Oxford University Press (OUP)

    Prof Kazutake Hirooka

  2. T7 expression system in Bacillus subtilis utilizing the rhiL promoter responsive to pectin and glucose

    Article • Bioscience Biotechnology and Biochemistry, March 2025, Oxford University Press (OUP)

    Prof Kazutake Hirooka

  3. Protein expression systems combining a flavonoid-inducible promoter and T7 RNA polymerase in Bacillus subtilis

    Article • Bioscience Biotechnology and Biochemistry, June 2023, Oxford University Press (OUP)

    Prof Kazutake Hirooka

  4. RhgKL and CcpA directly regulate the rhiLFGN-rhgR-yesTUV operon involved in the metabolism of rhamnogalacturonan type I in Bacillus subtilis

    Article • Bioscience Biotechnology and Biochemistry, July 2022, Oxford University Press (OUP)

    Prof Kazutake Hirooka

  5. Orphan Nuclear Receptor RORα Regulates Enzymatic Metabolism of Cerebral 24S-Hydroxycholesterol through CYP39A1 Intronic Response Element Activation

    Article • International Journal of Molecular Sciences, May 2020, MDPI AG

    Prof Kazutake Hirooka

  6. Identification of critical residues for the catalytic activity of ComQ, a Bacillus prenylation enzyme for quorum sensing, by using a simple bioassay system

    Article • Bioscience Biotechnology and Biochemistry, October 2019, Taylor & Francis

    Prof Kazutake Hirooka

  7. Bacillus subtilis highly efficient protein expression systems that are chromosomally integrated and controllable by glucose and rhamnose

    Article • Bioscience Biotechnology and Biochemistry, July 2018, Taylor & Francis

    Prof Kazutake Hirooka

  8. Dual Regulation of Bacillus subtilis kinB Gene Encoding a Sporulation Trigger by SinR through Transcription Repression and Positive Stringent Transcription Control

    Article • Frontiers in Microbiology, December 2017, Frontiers

    Prof Kazutake Hirooka

  9. rhamnose catabolism in a soil bacterium

    Article • Journal of Bacteriology, December 2015, ASM Journals

    Prof Kazutake Hirooka

  10. Transcriptional response machineries ofBacillus subtilisconducive to plant growth promotion

    Article • Bioscience Biotechnology and Biochemistry, September 2014, Taylor & Francis

    Prof Kazutake Hirooka

  11. CcpA-Mediated Catabolite Activation of the Bacillus subtilis ilv-leu Operon and Its Negation by Either CodY- or TnrA-Mediated Negative Regulation

    Article • Journal of Bacteriology, August 2014, ASM Journals

    Prof Kazutake Hirooka

  12. Structural Insights into the Low pH Adaptation of a Unique Carboxylesterase fromFerroplasma

    Article • Journal of Biological Chemistry, July 2014, American Society for Biochemistry & Molecular Biology (ASBMB)

    Prof Kazutake Hirooka

  13. Structural characterization of a ligand-bound form ofBacillus subtilisFadR involved in the regulation of fatty acid degradation

    Article • Proteins Structure Function and Bioinformatics, January 2014, Wiley

    Prof Kazutake Hirooka

  14. Expression of kinA and kinB of Bacillus subtilis, Necessary for Sporulation Initiation, Is under Positive Stringent Transcription Control

    Article • Journal of Bacteriology, February 2013, ASM Journals

    Prof Kazutake Hirooka

  15. Direct and Indirect Regulation of the ycnKJI Operon Involved in Copper Uptake through Two Transcriptional Repressors, YcnK and CsoR, in Bacillus subtilis

    Article • Journal of Bacteriology, August 2012, ASM Journals

    Prof Kazutake Hirooka

  16. Identification of Aromatic Residues Critical to the DNA Binding and Ligand Response of theBacillus subtilisQdoR (YxaF) Repressor Antagonized by Flavonoids

    Article • Bioscience Biotechnology and Biochemistry, July 2011, Taylor & Francis

    Prof Kazutake Hirooka

  17. Catabolite Repression of the Bacillus subtilis FadR Regulon, Which Is Involved in Fatty Acid Catabolism

    Article • Journal of Bacteriology, March 2011, ASM Journals

    Prof Kazutake Hirooka

  18. Excess Production ofBacillus subtilisQuercetin 2,3-Dioxygenase Affects Cell Viability in the Presence of Quercetin

    Article • Bioscience Biotechnology and Biochemistry, May 2010, Taylor & Francis

    Prof Kazutake Hirooka

  19. Heavy Involvement of Stringent Transcription Control Depending on the Adenine or Guanine Species of the Transcription Initiation Site in Glucose and Pyruvate Metabolism in Bacillus subtilis

    Article • Journal of Bacteriology, January 2010, ASM Journals

    Prof Kazutake Hirooka

  20. Efficient in vitro synthesis of cis-polyisoprenes using a thermostable cis-prenyltransferase from a hyperthermophilic archaeon Thermococcus kodakaraensis

    Article • Journal of Biotechnology, August 2009, Elsevier

    Prof Kazutake Hirooka

  21. Regulation of the Bacillus subtilis Divergent yetL and yetM Genes by a Transcriptional Repressor, YetL, in Response to Flavonoids

    Article • Journal of Bacteriology, March 2009, ASM Journals

    Prof Kazutake Hirooka

  22. Identification and Characterization of a Novel Multidrug Resistance Operon, mdtRP (yusOP), of Bacillus subtilis

    Article • Journal of Bacteriology, March 2009, ASM Journals

    Prof Kazutake Hirooka

  23. Molecular Mechanisms Underlying the Positive Stringent Response of the Bacillus subtilis ilv-leu Operon, Involved in the Biosynthesis of Branched-Chain Amino Acids

    Article • Journal of Bacteriology, July 2008, ASM Journals

    Prof Kazutake Hirooka

  24. Efficient synthesis of trans-polyisoprene compounds using two thermostable enzymes in an organic–aqueous dual-liquid phase system

    Article • Biochemical and Biophysical Research Communications, January 2008, Elsevier

    Prof Kazutake Hirooka

  25. Regulation of fatty acid metabolism in bacteria

    Article • Molecular Microbiology, November 2007, Wiley

    Prof Kazutake Hirooka

  26. Dual Regulation of the Bacillus subtilis Regulon Comprising the lmrAB and yxaGH Operons and yxaF Gene by Two Transcriptional Repressors, LmrA and YxaF, in Response to Flavonoids

    Article • Journal of Bacteriology, May 2007, ASM Journals

    Prof Kazutake Hirooka

  27. Organization and Function of the YsiA Regulon ofBacillus subtilisInvolved in Fatty Acid Degradation

    Article • Journal of Biological Chemistry, December 2006, American Society for Biochemistry & Molecular Biology (ASBMB)

    Prof Kazutake Hirooka

  28. Enhancement of Glutamine Utilization in Bacillus subtilis through the GlnK-GlnL Two-Component Regulatory System

    Article • Journal of Bacteriology, July 2005, ASM Journals

    Prof Kazutake Hirooka

  29. Enzymatic and structural characterization of type II isopentenyl diphosphate isomerase from hyperthermophilic archaeon Thermococcus kodakaraensis

    Article • Biochemical and Biophysical Research Communications, June 2005, Elsevier

    Prof. Dr. Masood Ahmed Siddiqui, Prof Kazutake Hirooka

  30. Elaborate transcription regulation of theBacillus subtilis ilv-leuoperon involved in the biosynthesis of branched-chain amino acids through global regulators of CcpA, CodY and TnrA

    Article • Molecular Microbiology, April 2005, Wiley

    Prof Kazutake Hirooka

  31. Functional Analysis of Two Solanesyl Diphosphate Synthases fromArabidopsis thaliana

    Article • Bioscience Biotechnology and Biochemistry, January 2005, Taylor & Francis

    Prof Kazutake Hirooka

  32. Negative Transcriptional Regulation of the ilv-leu Operon for Biosynthesis of Branched-Chain Amino Acids through the Bacillus subtilis Global Regulator TnrA

    Article • Journal of Bacteriology, December 2004, ASM Journals

    Prof Kazutake Hirooka

  33. Temperature-dependent modulation of farnesyl diphosphate/geranylgeranyl diphosphate synthase from hyperthermophilic archaea

    Article • Biochemical and Biophysical Research Communications, December 2004, Elsevier

    Prof Kazutake Hirooka

  34. Cloning and Characterization of Farnesyl Diphosphate Synthase from the Rubber-Producing MushroomLactarius chrysorrheus

    Article • Bioscience Biotechnology and Biochemistry, January 2004, Taylor & Francis

    Prof Kazutake Hirooka

  35. Molecular cloning and characterization of a thermostable carboxylesterase from an archaeon, Sulfolobus shibatae DSM5389: Non-linear kinetic behavior of a hormone-sensitive lipase family enzyme

    Article • Journal of Bioscience and Bioengineering, January 2004, Elsevier

    Prof Kazutake Hirooka

  36. Cloning and kinetic characterization of Arabidopsis thaliana solanesyl diphosphate synthase

    Article • Biochemical Journal, March 2003, Portland Press Ltd.

    Prof Kazutake Hirooka

  37. Dramatic changes in the substrate specificities of prenyltransferase by a single amino acid substitution

    Article • Journal of Molecular Catalysis B Enzymatic, December 2002, Elsevier

    Prof Kazutake Hirooka

  38. The role of histidine-114 ofSulfolobus acidocaldariusgeranylgeranyl diphosphate synthase in chain-length determination

    Article • FEBS Letters, September 2000, Wiley

    Prof Kazutake Hirooka

  39. Mechanism of product chain length determination for heptaprenyl diphosphate synthase from Bacillus stearothermophilus

    Article • European Journal of Biochemistry, July 2000, Wiley

    Prof Kazutake Hirooka

  40. A Pathway Where Polyprenyl Diphosphate Elongates in Prenyltransferase

    Article • Journal of Biological Chemistry, October 1998, American Society for Biochemistry & Molecular Biology (ASBMB)

    Prof Kazutake Hirooka

  41. Conversion from Archaeal Geranylgeranyl Diphosphate Synthase to Farnesyl Diphosphate Synthase

    Article • Journal of Biological Chemistry, February 1997, American Society for Biochemistry & Molecular Biology (ASBMB)

    Prof Kazutake Hirooka

  42. Conversion of Product Specificity of Archaebacterial Geranylgeranyl-diphosphate Synthase

    Article • Journal of Biological Chemistry, August 1996, American Society for Biochemistry & Molecular Biology (ASBMB)

    Prof Kazutake Hirooka