All Stories

  1. Membranous and Membraneless Interfaces—Origins of Artificial Cellular Complexity

    Article • Life, July 2023, MDPI AG

    Kanta Tsumoto

  2. Microscopic Observation of Membrane Fusion between Giant Liposomes and Baculovirus Budded Viruses Activated by the Release of a Caged Proton

    Article • Membranes, May 2023, MDPI AG

    Kanta Tsumoto

  3. Self-emergent vortex flow of microtubule and kinesin in cell-sized droplets under water/water phase separation

    Article • Communications Chemistry, April 2023, Springer Science + Business Media

    Kanta Tsumoto, Professor Kenichi Yoshikawa

  4. Front Cover: Water‐in‐Water Droplets Selectively Uptake Self‐Assembled DNA Nano/Microstructures: a Versatile Method for Purification in DNA Nanotechnology (ChemBioChem 17/2022)

    Article • ChemBioChem, July 2022, Wiley

    Kanta Tsumoto

  5. Water‐in‐Water Droplets Selectively Uptake Self‐Assembled DNA Nano/Microstructures: a Versatile Method for Purification in DNA Nanotechnology

    Article • ChemBioChem, July 2022, Wiley

    Professor Kenichi Yoshikawa, Kanta Tsumoto

  6. Mechanism of Budded Virus Envelope Fusion into a Planar Bilayer Lipid Membrane on a SiO2 Substrate

    Article • Langmuir, April 2022, American Chemical Society (ACS)

    Kanta Tsumoto

  7. Electrofusion of cells with different diameters by generating asymmetrical electric field in the microwell array

    Article • Analytical Sciences, February 2022, Springer Science + Business Media

    Kanta Tsumoto

  8. Class-Switching of B Lymphocytes by DNA and Cell Immunization for Stereospecific Monoclonal Antibodies against Native GPCR

    Article • Immuno, November 2021, MDPI AG

    Kanta Tsumoto

  9. Conformation-specific monoclonal antibodies recognizing the native structure of G protein-coupled receptor (GPCR)

    Article • International Immunopharmacology, September 2021, Elsevier

    Kanta Tsumoto

  10. Polymerization/depolymerization of actin cooperates with the morphology and stability of cell-sized droplets generated in a polymer solution under a depletion effect

    Article • The Journal of Chemical Physics, August 2021, American Institute of Physics

    Kingo Takiguchi, Professor Kenichi Yoshikawa, Kanta Tsumoto

  11. Cover Feature: Self‐Emergent Protocells Generated in an Aqueous Solution with Binary Macromolecules through Liquid‐Liquid Phase Separation (ChemBioChem 23/2020)

    Article • ChemBioChem, November 2020, Wiley

    Kanta Tsumoto

  12. Optimization of stereospecific targeting technique for selective production of monoclonal antibodies against native ephrin type-A receptor 2

    Article • Journal of Immunological Methods, September 2020, Elsevier

    Kanta Tsumoto

  13. Self‐Emergent Protocells Generated in an Aqueous Solution with Binary Macromolecules through Liquid‐Liquid Phase Separation

    Article • ChemBioChem, August 2020, Wiley

    Professor Kenichi Yoshikawa, Kanta Tsumoto

  14. Nonspecific characteristics of macromolecules create specific effects in living cells

    Article • Biophysical Reviews, March 2020, Springer Science + Business Media

    Professor Kenichi Yoshikawa, Kanta Tsumoto

  15. Membrane fusion and infection abilities of baculovirus virions are preserved during freezing and thawing in the presence of trehalose

    Article • Bioscience Biotechnology and Biochemistry, December 2019, Taylor & Francis

    Kanta Tsumoto

  16. B-cell receptor-based multitargeting method for simultaneous production of novel multiple monoclonal antibodies

    Article • Journal of Bioscience and Bioengineering, November 2019, Elsevier

    Kanta Tsumoto

  17. Aqueous/Aqueous Micro Phase Separation: Construction of an Artificial Model of Cellular Assembly

    Article • Frontiers in Chemistry, February 2019, Frontiers

    Kanta Tsumoto, Professor Kenichi Yoshikawa

  18. Future perspectives of therapeutic monoclonal antibodies

    Article • Immunotherapy, February 2019, Future Medicine

    Kanta Tsumoto

  19. Specific Spatial Localization of Actin and DNA in a Water/Water Microdroplet: Self-Emergence of a Cell-Like Structure

    Article • ChemBioChem, June 2018, Wiley

    Professor Kenichi Yoshikawa, Kanta Tsumoto

  20. Specific Spatial Localization of Actin and DNA in a Water/Water Microdroplet: Self-Emergence of a Cell-Like Structure

    Article • ChemBioChem, June 2018, Wiley

    Professor Kenichi Yoshikawa, Kanta Tsumoto

  21. A reverse-phase method revisited: Rapid high-yield preparation of giant unilamellar vesicles (GUVs) using emulsification followed by centrifugation

    Article • Colloids and Surfaces A Physicochemical and Engineering Aspects, June 2018, Elsevier

    Kanta Tsumoto

  22. Opposite effect of polyamines on In vitro gene expression: Enhancement at low concentrations but inhibition at high concentrations

    Article • PLoS ONE, March 2018, PLOS

    Professor Kenichi Yoshikawa, Kanta Tsumoto

  23. Huntingtin Polyglutamine-Dependent Protein Aggregation in Reconstituted Cells

    Article • ACS Synthetic Biology, December 2017, American Chemical Society (ACS)

    Kanta Tsumoto

  24. Membrane fusion between baculovirus budded virus-enveloped particles and giant liposomes generated using a droplet-transfer method for the incorporation of recombinant membrane proteins

    Article • Colloids and Surfaces B Biointerfaces, July 2017, Elsevier

    Kanta Tsumoto

  25. The Aqueous Two Phase System (ATPS) Deserves Plausible Real-World Modeling for the Structure and Function of Living Cells

    Article • MRS Advances, January 2017, Cambridge University Press

    Kanta Tsumoto, Professor Kenichi Yoshikawa

  26. The method used to culture host cells (Sf9 cells) can affect the qualities of baculovirus budding particles expressing recombinant proteins

    Article • Bioscience Biotechnology and Biochemistry, March 2016, Taylor & Francis

    Kanta Tsumoto

  27. Does DNA Exert an Active Role in Generating Cell-Sized Spheres in an Aqueous Solution with a Crowding Binary Polymer?

    Article • Life, February 2015, MDPI AG

    Professor Kenichi Yoshikawa, Kanta Tsumoto

  28. Incorporation of adenylate cyclase into membranes of giant liposomes using membrane fusion with recombinant baculovirus-budded virus particles

    Article • Biotechnology Letters, February 2014, Springer Science + Business Media

    Kanta Tsumoto

  29. Crowding by Anionic Nanoparticles Causes DNA Double-Strand Instability and Compaction

    Article • The Journal of Physical Chemistry, February 2014, American Chemical Society (ACS)

    Dr Anatoly Zinchenko, Professor Kenichi Yoshikawa, Kanta Tsumoto

  30. pH Switching That Crosses over the Isoelectric Point (pI) Can Improve the Entrapment of Proteins within Giant Liposomes by Enhancing Protein–Membrane Interaction

    Article • Langmuir, January 2014, American Chemical Society (ACS)

    Kanta Tsumoto

  31. The binding of soluble recombinant human Fcγ receptor I for human immunoglobulin G is conferred by its first and second extracellular domains

    Article • Molecular Immunology, July 2013, Elsevier

    Kanta Tsumoto

  32. Monoclonal antibodies based on hybridoma technology

    Article • Pharmaceutical Patent Analyst, March 2013, Future Science

    Kanta Tsumoto

  33. Engineering of recombinant human Fc  receptor I by directed evolution

    Article • Protein Engineering Design and Selection, September 2012, Oxford University Press (OUP)

    Kanta Tsumoto

  34. Efficient expression of recombinant soluble human FcγRI in mammalian cells and its characterization

    Article • Protein Expression and Purification, March 2012, Elsevier

    Kanta Tsumoto

  35. Cadherin-integrated liposomes with potential application in a drug delivery system

    Article • Biomaterials, December 2011, Elsevier

    Kanta Tsumoto

  36. Giant liposomes as microcapsules with large trapping volumes: Downsizing through various membrane filters and analysis with a calcein quenching method

    Article • November 2011, Institute of Electrical & Electronics Engineers (IEEE)

    Kanta Tsumoto

  37. Monitoring of membrane collapse and enzymatic reaction with single giant liposomes embedded in agarose gel

    Article • Colloid & Polymer Science, June 2011, Springer Science + Business Media

    Kanta Tsumoto

  38. Hybridoma technologies for antibody production

    Article • Immunotherapy, March 2011, Future Medicine

    Kanta Tsumoto

  39. Construction of an In Vitro Model of a Living Cellular System

    Article • October 2010, Springer Science + Business Media

    Professor Kenichi Yoshikawa, Kanta Tsumoto

  40. Confocal microscopic observation of fusion between baculovirus budded virus envelopes and single giant unilamellar vesicles

    Article • Biochimica et Biophysica Acta (BBA) - Biomembranes, September 2010, Elsevier

    Kanta Tsumoto

  41. Preparation of connexin43-integrated giant Liposomes by a baculovirus expression-liposome fusion method

    Article • Biotechnology and Bioengineering, June 2010, Wiley

    Kanta Tsumoto

  42. Recent Advances in Antigen-Based Generation of Monoclonal Antibodies

    Article • Current Immunology Reviews, February 2010, Bentham Science Publishers

    Kanta Tsumoto

  43. Diagnosis and discrimination of autoimmune Graves' disease and Hashimoto's disease using thyroid-stimulating hormone receptor-containing recombinant proteoliposomes

    Article • Journal of Bioscience and Bioengineering, December 2009, Elsevier

    Kanta Tsumoto

  44. G protein coupled receptors (GPCRs) reconstituted on recombinant proteoliposomes using baculovirus-liposome membrane fusion

    Article • November 2009, Institute of Electrical & Electronics Engineers (IEEE)

    Kanta Tsumoto

  45. Efficient formation of giant liposomes through the gentle hydration of phosphatidylcholine films doped with sugar

    Article • Colloids and Surfaces B Biointerfaces, January 2009, Elsevier

    Kanta Tsumoto

  46. Recombinant Proteoliposomes Prepared Using Baculovirus Expression Systems

    Article • January 2009, Elsevier

    Kanta Tsumoto

  47. Reconstitution and Microscopic Observation of G Protein Subunits on Giant Liposomes: Attempt to Construct a Cell Model with Functional Membrane Protein Components

    Article • November 2008, Institute of Electrical & Electronics Engineers (IEEE)

    Kanta Tsumoto

  48. Development of a Novel Preparation Method of Recombinant Proteoliposomes Using Baculovirus Gene Expression Systems

    Article • The Journal of Biochemistry, September 2008, Oxford University Press (OUP)

    Kanta Tsumoto

  49. Display of Recombinant Membrane Receptors on Giant Liposomes: Attempt to Construct a Cell Model with Integrated Membrane Protein Systems

    Article • November 2007, Institute of Electrical & Electronics Engineers (IEEE)

    Kanta Tsumoto

  50. Unbinding of lipid bilayers induced by osmotic pressure in relation to unilamellar vesicle formation

    Article • EPL (Europhysics Letters), October 2007, Institute of Physics Publishing

    Kanta Tsumoto

  51. Genetic Nanomedicine and Tissue Engineering

    Article • Medical Clinics of North America, September 2007, Elsevier

    Kanta Tsumoto

  52. Membrane Fusion between a Giant Vesicle and Small Enveloped Particles: Possibilities for the Application to Construct Model Cells

    Article • January 2006, Institute of Electrical & Electronics Engineers (IEEE)

    Kanta Tsumoto

  53. Enhancement and inhibition of DNA transcriptional activity by spermine: A marked difference between linear and circular templates

    Article • FEBS Letters, August 2005, Wiley

    Professor Kenichi Yoshikawa, Kanta Tsumoto

  54. All-or-none switching of transcriptional activity on single DNA molecules caused by a discrete conformational transition

    Article • Applied Physics Letters, May 2005, American Institute of Physics

    Professor Kenichi Yoshikawa, Kanta Tsumoto

  55. Gene Expression within Cell-Sized Lipid Vesicles

    Article • ChemBioChem, November 2003, Wiley

    Professor Kenichi Yoshikawa, Kanta Tsumoto

  56. Giant DNA molecules exhibit on/off switching of transcriptional activity through conformational transition

    Article • Biophysical Chemistry, October 2003, Elsevier

    Professor Kenichi Yoshikawa, Kanta Tsumoto

  57. NTP Concentration Switches Transcriptional Activity by Changing the Large-Scale Structure of DNA

    Article • Biomacromolecules, September 2003, American Chemical Society (ACS)

    Professor Kenichi Yoshikawa, Kanta Tsumoto

  58. Folding transition of large DNA completely inhibits the action of a restriction endonuclease as revealed by single-chain observation

    Article • FEBS Letters, September 2002, Wiley

    Professor Kenichi Yoshikawa, Kanta Tsumoto

  59. Switching of Higher-Order Structure of DNA and Gene Expression.

    Article • Seibutsu Butsuri, January 2002, Biophysical Society of Japan

    Kanta Tsumoto

  60. Giant Liposome as a Biochemical Reactor:  Transcription of DNA and Transportation by Laser Tweezers

    Article • Langmuir, November 2001, American Chemical Society (ACS)

    Professor Kenichi Yoshikawa, Kanta Tsumoto

  61. Intra-molecular phase segregation in a single polyelectrolyte chain

    Article • The Journal of Chemical Physics, April 2001, American Institute of Physics

    Professor Kenichi Yoshikawa, Kanta Tsumoto

  62. RNA switches the higher-order structure of DNA

    Article • Biophysical Chemistry, November 1999, Elsevier

    Professor Kenichi Yoshikawa, Kanta Tsumoto

  63. DNA conformation and transcriptional properties: a higher-order of silence.

    Article • Institute of Electrical & Electronics Engineers (IEEE)

    Professor Kenichi Yoshikawa, Kanta Tsumoto