All Stories

  1. Deletion of DNA ligase IV homolog confers higher gene targeting efficiency on homologous recombination in Komagataella phaffii

    Article • FEMS Yeast Research, July 2018, Oxford University Press (OUP)

    Dr Jun Ishii

  2. A Stable, Autonomously Replicating Plasmid Vector Containing Pichia pastoris Centromeric DNA

    Article • Applied and Environmental Microbiology, May 2018, ASM Journals

    Dr Jun Ishii

  3. Modifying Expression Modes of Human Neurotensin Receptor Type 1 Alters Sensing Capabilities for Agonists in Yeast Signaling Biosensor

    Article • Biotechnology Journal, November 2017, Wiley

    Yasuyuki Nakamura, Dr Jun Ishii

  4. Positive Detection of GPCR Antagonists Using a System for Inverted Expression of a Fluorescent Reporter Gene

    Article • ACS Synthetic Biology, May 2017, American Chemical Society (ACS)

    Dr Jun Ishii

  5. Positive Feedback Genetic Circuit Incorporating a Constitutively Active Mutant Gal3 into Yeast GAL Induction System

    Article • ACS Synthetic Biology, March 2017, American Chemical Society (ACS)

    Dr Jun Ishii

  6. Expression of varied GFPs in Saccharomyces cerevisiae: codon optimization yields stronger than expected expression and fluorescence intensity

    Article • Scientific Reports, October 2016, Springer Science + Business Media

    Dr Jun Ishii

  7. Enhancement of astaxanthin production in Xanthophyllomyces dendrorhous by efficient method for the complete deletion of genes

    Article • Microbial Cell Factories, September 2016, Springer Science + Business Media

    Dr Jun Ishii

  8. From mannan to bioethanol: cell surface co-display of β-mannanase and β-mannosidase on yeast Saccharomyces cerevisiae

    Article • Biotechnology for Biofuels, September 2016, Springer Science + Business Media

    Dr Jun Ishii

  9. Continuous crossbreeding of sake yeasts using growth selection systems for a-type and α-type cells

    Article • AMB Express, July 2016, Springer Science + Business Media

    Dr Jun Ishii

  10. Overexpressing enzymes of the Ehrlich pathway and deleting genes of the competing pathway in Saccharomyces cerevisiae for increasing 2-phenylethanol production from glucose

    Article • Journal of Bioscience and Bioengineering, July 2016, Elsevier

    Dr Jun Ishii

  11. Enhanced cell-surface display and secretory production of cellulolytic enzymes withSaccharomyces cerevisiaeSed1 signal peptide

    Article • Biotechnology and Bioengineering, June 2016, Wiley

    Dr Jun Ishii

  12. Current Techniques for Studying Oligomer Formations of G-Protein-Coupled Receptors Using Mammalian and Yeast Cells

    Article • Current Medicinal Chemistry, May 2016, Bentham Science Publishers

    Dr Jun Ishii

  13. Dual-color reporter switching system to discern dimer formations of G-protein-coupled receptors using Cre/loxP site-specific recombination in yeast

    Article • Biotechnology and Bioengineering, March 2016, Wiley

    Dr Jun Ishii

  14. Identification of a novel hedycaryol synthase gene isolated from Camellia brevistyla flowers and floral scent of Camellia cultivars

    Article • Planta, January 2016, Springer Science + Business Media

    Dr Jun Ishii

  15. Rational design and evolutional fine tuning of Saccharomyces cerevisiae for biomass breakdown

    Article • Current Opinion in Chemical Biology, December 2015, Elsevier

    Dr Jun Ishii

  16. Gγ recruitment systems specifically select PPI and affinity-enhanced candidate proteins that interact with membrane protein targets

    Article • Scientific Reports, November 2015, Springer Science + Business Media

    Dr Jun Ishii

  17. FudC, a protein primarily responsible for furfural detoxification in Corynebacterium glutamicum

    Article • Applied Microbiology and Biotechnology, November 2015, Springer Science + Business Media

    Dr Jun Ishii

  18. Applications of yeast-based signaling sensor for characterization of antagonist and analysis of site-directed mutants of the human serotonin 1A receptor

    Article • Biotechnology and Bioengineering, June 2015, Wiley

    Dr Jun Ishii

  19. Eliminating the isoleucine biosynthetic pathway to reduce competitive carbon outflow during isobutanol production by Saccharomyces cerevisiae

    Article • Microbial Cell Factories, April 2015, Springer Science + Business Media

    Dr Jun Ishii

  20. Improving the odorant sensitivity of olfactory receptor-expressing yeast with accessory proteins

    Article • Analytical Biochemistry, February 2015, Elsevier

    Dr Jun Ishii

  21. Polyamino acid display on cell surfaces enhances salt and alcohol tolerance of Escherichia coli

    Article • Biotechnology Letters, January 2015, Springer Science + Business Media

    Dr Jun Ishii

  22. The mapping of yeast's G-protein coupled receptor with an atomic force microscope

    Article • Nanoscale, January 2015, Royal Society of Chemistry

    Dr Jun Ishii

  23. Rational design of a degradable polyanion for layer-by-layer assembly for encapsulation and release of cationic functional biomolecules

    Article • Chemical Communications, January 2015, Royal Society of Chemistry

    Tatsuo Maruyama, Dr Jun Ishii

  24. Mutation of arginine residues to avoid non-specific cellular uptakes for hepatitis B virus core particles

    Article • Journal of Nanobiotechnology, January 2015, Springer Science + Business Media

    Dr Jun Ishii

  25. Efficient co-displaying and artificial ratio control of α-amylase and glucoamylase on the yeast cell surface by using combinations of different anchoring domains

    Article • Applied Microbiology and Biotechnology, November 2014, Springer Science + Business Media

    Dr Jun Ishii

  26. Desired Alteration of Protein Affinities: Competitive Selection of Protein Variants Using Yeast Signal Transduction Machinery

    Article • PLoS ONE, September 2014, PLOS

    Dr Jun Ishii

  27. Genetic Engineering of Bio-Nanoparticles for Drug Delivery: A Review

    Article • Journal of Biomedical Nanotechnology, September 2014, American Scientific Publishers

    Dr Jun Ishii

  28. Detoxification of furfural in Corynebacterium glutamicum under aerobic and anaerobic conditions

    Article • Applied Microbiology and Biotechnology, August 2014, Springer Science + Business Media

    Dr Jun Ishii

  29. Construction of a yeast-based signaling biosensor for human angiotensin II type 1 receptor via functional coupling between Asn295-mutated receptor and Gpa1/Gi3chimeric Gα

    Article • Biotechnology and Bioengineering, July 2014, Wiley

    Dr Jun Ishii

  30. Three gene expression vector sets for concurrently expressing multiple genes inSaccharomyces cerevisiae

    Article • FEMS Yeast Research, February 2014, Oxford University Press (OUP)

    Dr Jun Ishii

  31. Microbial fluorescence sensing for human neurotensin receptor type 1 using Gα-engineered yeast cells

    Article • Analytical Biochemistry, February 2014, Elsevier

    Dr Jun Ishii

  32. A display of pH-sensitive fusogenic GALA peptide facilitates endosomal escape from a Bio-nanocapsule via an endocytic uptake pathway

    Article • Journal of Nanobiotechnology, January 2014, Springer Science + Business Media

    Dr Jun Ishii

  33. Bright Fluorescence Monitoring System Utilizing Zoanthus sp. Green Fluorescent Protein (ZsGreen) for Human G-Protein-Coupled Receptor Signaling in Microbial Yeast Cells

    Article • PLoS ONE, December 2013, PLOS

    Dr Jun Ishii

  34. Simultaneous method for analyzing dimerization and signaling of G-protein-coupled receptor in yeast by dual-color reporter system

    Article • Biotechnology and Bioengineering, October 2013, Wiley

    Dr Jun Ishii

  35. Regulation of central carbon metabolism in Saccharomyces cerevisiae by metabolic inhibitors

    Article • Journal of Bioscience and Bioengineering, July 2013, Elsevier

    Dr Jun Ishii

  36. Rapid, Facile Detection of Heterodimer Partners for Target Human G-Protein-Coupled Receptors Using a Modified Split-Ubiquitin Membrane Yeast Two-Hybrid System

    Article • PLoS ONE, June 2013, PLOS

    Dr Jun Ishii

  37. A review of enzymes and microbes for lignocellulosic biorefinery and the possibility of their application to consolidated bioprocessing technology

    Article • Bioresource Technology, May 2013, Elsevier

    Dr Jun Ishii

  38. An affinity chromatography method used to purify His-tag-displaying bio-nanocapsules

    Article • Journal of Virological Methods, May 2013, Elsevier

    Dr Jun Ishii

  39. Development of microbial cell factories for bio-refinery through synthetic bioengineering

    Article • Journal of Biotechnology, January 2013, Elsevier

    Dr Jun Ishii

  40. Increased isobutanol production in Saccharomyces cerevisiae by eliminating competing pathways and resolving cofactor imbalance

    Article • Microbial Cell Factories, January 2013, Springer Science + Business Media

    Dr Jun Ishii

  41. Targeting cancer cell-specific RNA interference by siRNA delivery using a complex carrier of affibody-displaying bio-nanocapsules and liposomes

    Article • Journal of Nanobiotechnology, January 2013, Springer Science + Business Media

    Dr Jun Ishii

  42. Granting specificity for breast cancer cells using a hepatitis B core particle with a HER2-targeted affibody molecule

    Article • The Journal of Biochemistry, December 2012, Oxford University Press (OUP)

    Dr Jun Ishii

  43. Construction of an Artificial Pathway for Isobutanol Biosynthesis in the Cytosol ofSaccharomyces cerevisiae

    Article • Bioscience Biotechnology and Biochemistry, November 2012, Taylor & Francis

    Dr Jun Ishii

  44. Complex carriers of affibody-displaying bio-nanocapsules and composition-varied liposomes for HER2-expressing breast cancer cell-specific protein delivery

    Article • Journal of Drug Targeting, October 2012, Taylor & Francis

    Dr Jun Ishii

  45. Reduction of furan derivatives by overexpressing NADH-dependent Adh1 improves ethanol fermentation using xylose as sole carbon source with Saccharomyces cerevisiae harboring XR–XDH pathway

    Article • Applied Microbiology and Biotechnology, September 2012, Springer Science + Business Media

    Dr Jun Ishii

  46. Implementation of a transhydrogenase-like shunt to counter redox imbalance during xylose fermentation in Saccharomyces cerevisiae

    Article • Applied Microbiology and Biotechnology, August 2012, Springer Science + Business Media

    Dr Jun Ishii

  47. An improved bioluminescence‐based signaling assay for odor sensing with a yeast expressing a chimeric olfactory receptor

    Article • Biotechnology and Bioengineering, July 2012, Wiley

    Professor Masafumi Yohda, Dr Jun Ishii

  48. Improved identification of agonist-mediated Gαi-specific human G-protein-coupled receptor signaling in yeast cells by flow cytometry

    Article • Analytical Biochemistry, July 2012, Elsevier

    Dr Jun Ishii

  49. Cell Wall Trapping of Autocrine Peptides for Human G-Protein-Coupled Receptors on the Yeast Cell Surface

    Article • PLoS ONE, May 2012, PLOS

    Dr Jun Ishii

  50. Genetic engineering to enhance the Ehrlich pathway and alter carbon flux for increased isobutanol production from glucose by Saccharomyces cerevisiae

    Article • Journal of Biotechnology, May 2012, Elsevier

    Dr Jun Ishii

  51. Transplantation of the GAL regulon into G-protein signaling circuitry in yeast

    Article • Analytical Biochemistry, May 2012, Elsevier

    Dr Jun Ishii

  52. An energy-saving glutathione production method from low-temperature cooked rice using amylase-expressing Saccharomyces cerevisiae

    Article • Biotechnology Journal, February 2012, Wiley

    Dr Jun Ishii

  53. Protein-encapsulated bio-nanocapsules production with ER membrane localization sequences

    Article • Journal of Biotechnology, January 2012, Elsevier

    Dr Jun Ishii

  54. Amplification of agonist stimulation of human G-protein-coupled receptor signaling in yeast

    Article • Analytical Biochemistry, October 2011, Elsevier

    Dr Jun Ishii

  55. The N‐terminal replacement of an olfactory receptor for the development of a Yeast‐based biomimetic odor sensor

    Article • Biotechnology and Bioengineering, September 2011, Wiley

    Professor Masafumi Yohda, Dr Jun Ishii

  56. Gγ recruitment system incorporating a novel signal amplification circuit to screen transient protein-protein interactions

    Article • FEBS Journal, August 2011, Wiley

    Dr Jun Ishii

  57. Engineering strategy of yeast metabolism for higher alcohol production

    Article • Microbial Cell Factories, January 2011, Springer Science + Business Media

    Dr Jun Ishii

  58. Metabolic pathway engineering based on metabolomics confers acetic and formic acid tolerance to a recombinant xylose-fermenting strain of Saccharomyces cerevisiae

    Article • Microbial Cell Factories, January 2011, Springer Science + Business Media

    Dr Jun Ishii

  59. Construction of a xylose-metabolizing yeast by genome integration of xylose isomerase gene and investigation of the effect of xylitol on fermentation

    Article • Applied Microbiology and Biotechnology, September 2010, Springer Science + Business Media

    Dr Jun Ishii

  60. Protein-protein interactions and selection: yeast-based approaches that exploit guanine nucleotide-binding protein signaling

    Article • FEBS Journal, April 2010, Wiley

    Dr Jun Ishii

  61. Control of signalling properties of human somatostatin receptor subtype-5 by additional signal sequences on its amino-terminus in yeast

    Article • The Journal of Biochemistry, March 2010, Oxford University Press (OUP)

    Dr Jun Ishii

  62. Importance of asparagine residues at positions 13 and 26 on the amino-terminal domain of human somatostatin receptor subtype-5 in signalling

    Article • The Journal of Biochemistry, March 2010, Oxford University Press (OUP)

    Dr Jun Ishii

  63. The competitor-introduced Gγ recruitment system, a new approach for screening affinity-enhanced proteins

    Article • FEBS Journal, March 2010, Wiley

    Dr Jun Ishii

  64. Displaying non-natural, functional molecules on yeast surfaces via biotin–streptavidin interaction

    Article • Journal of Biotechnology, January 2010, Elsevier

    Dr Jun Ishii

  65. Construction of a novel detection system for protein-protein interactions using yeast G-protein signaling

    Article • FEBS Journal, May 2009, Wiley

    Dr Jun Ishii

  66. A Simple and Immediate Method for Simultaneously Evaluating Expression Level and Plasmid Maintenance in Yeast

    Article • The Journal of Biochemistry, February 2009, Oxford University Press (OUP)

    Dr Jun Ishii

  67. Development of a yeast protein fragment complementation assay (PCA) system using dihydrofolate reductase (DHFR) with specific additives

    Article • Applied Microbiology and Biotechnology, August 2008, Springer Science + Business Media

    Dr Jun Ishii

  68. Rapid and Efficient Selection of Yeast Displaying a Target Protein Using Thermo-responsive Magnetic Nanoparticles

    Article • Biotechnology Progress, April 2008, Wiley

    Dr Jun Ishii

  69. Yeast-Based Fluorescence Reporter Assay of G Protein-coupled Receptor Signalling for Flow Cytometric Screening: FAR1-Disruption Recovers Loss of Episomal Plasmid Caused by Signalling in Yeast

    Article • The Journal of Biochemistry, January 2008, Oxford University Press (OUP)

    Dr Jun Ishii

  70. High-efficiency recovery of target cells using improved yeast display system for detection of protein–protein interactions

    Article • Applied Microbiology and Biotechnology, May 2007, Springer Science + Business Media

    Dr Jun Ishii

  71. Construction of a novel synergistic system for production and recovery of secreted recombinant proteins by the cell surface engineering

    Article • Applied Microbiology and Biotechnology, March 2007, Springer Science + Business Media

    Dr Jun Ishii

  72. Quantitative and Dynamic Analyses of G Protein-Coupled Receptor Signaling in Yeast Using Fus1, Enhanced Green Fluorescence Protein (EGFP), and His3 Fusion Protein

    Article • Biotechnology Progress, August 2006, Wiley

    Dr Jun Ishii