All Stories

  1. EatA mediated degradation of intestinal mucus is species-specific and driven by MUC2 structural features

    Article • Nature Communications, December 2025, Springer Science + Business Media

    Professor James Michael Fleckenstein

  2. ETEC heat-labile toxin promotes β-catenin stabilization and transcriptional reprogramming to disrupt intestinal epithelial differentiation

    Article • October 2025, Cold Spring Harbor Laboratory Press

    Professor James Michael Fleckenstein

  3. Parenteral vaccination with recombinant EtpA glycoprotein impairs enterotoxigenic E. coli colonization

    Article • Infection and Immunity, June 2025, ASM Journals

    Professor James Michael Fleckenstein

  4. Degradation of the intestinal mucus layer by the ETEC protease EatA is species specific determined by the structure of the MUC2 mucin

    Article • March 2025, Cold Spring Harbor Laboratory Press

    Professor James Michael Fleckenstein

  5. Parenteral Vaccination with recombinant EtpA glycoprotein impairs enterotoxigenic E. coli colonization

    Article • January 2025, Cold Spring Harbor Laboratory Press

    Professor James Michael Fleckenstein

  6. Repeat modules and N-linked glycans define structure and antigenicity of a critical enterotoxigenic E. coli adhesin

    Article • PLoS Pathogens, September 2024, PLOS

    Professor James Michael Fleckenstein

  7. Host-derived CEACAM-laden vesicles engage enterotoxigenic Escherichia coli for elimination and toxin neutralization

    Article • Proceedings of the National Academy of Sciences, September 2024, Proceedings of the National Academy of Sciences

    Professor James Michael Fleckenstein

  8. Structural and biophysical characterization of the secreted, β-helical adhesin EtpA of Enterotoxigenic Escherichia coli

    Article • PLOS One, June 2023, PLOS

    Professor James Michael Fleckenstein

  9. Interactions of pathogenic Escherichia coli with CEACAMs

    Article • Frontiers in Immunology, February 2023, Frontiers

    Professor James Michael Fleckenstein

  10. Spontaneous Bacterial Peritonitis Caused by Bordetella hinzii

    Article • Emerging Infectious Diseases, November 2021, Centers for Disease Control and Prevention (CDC)

    Professor James Michael Fleckenstein

  11. Confronting Challenges to Enterotoxigenic Escherichia coli Vaccine Development

    Article • Frontiers in Tropical Diseases, September 2021, Frontiers

    Professor James Michael Fleckenstein

  12. Changing the locks on intestinal signaling

    Article • Cell Host & Microbe, September 2021, Elsevier

    Professor James Michael Fleckenstein

  13. Effect of chronic alcohol exposure on gut vitamin B7 uptake: involvement of epigenetic mechanisms and effect of alcohol metabolites

    Article • AJP Gastrointestinal and Liver Physiology, August 2021, American Physiological Society

    Professor James Michael Fleckenstein

  14. Emerging Themes in the Molecular Pathogenesis of Enterotoxigenic Escherichia coli

    Article • The Journal of Infectious Diseases, July 2021, Oxford University Press (OUP)

    Professor James Michael Fleckenstein

  15. Vaccines for Protecting Infants from Bacterial Causes of Diarrheal Disease

    Article • Microorganisms, June 2021, MDPI AG

    Professor James Michael Fleckenstein

  16. Acute Bacterial Gastroenteritis

    Article • Gastroenterology Clinics of North America, June 2021, Elsevier

    Professor James Michael Fleckenstein

  17. Contribution of Noncanonical Antigens to Virulence and Adaptive Immunity in Human Infection with Enterotoxigenic E. coli

    Article • Infection and Immunity, April 2021, ASM Journals

    Professor James Michael Fleckenstein

  18. The Intriguing Interaction of Escherichia coli with the Host Environment and Innovative Strategies To Interfere with Colonization: a Summary of the 2019 E. coli and the Mucosal Immune System Meeting

    Article • Applied and Environmental Microbiology, November 2020, ASM Journals

    Dr Kurt Hanevik, Professor James Michael Fleckenstein

  19. CEACAMs serve as toxin-stimulated receptors for enterotoxigenic Escherichia coli

    Article • Proceedings of the National Academy of Sciences, November 2020, Proceedings of the National Academy of Sciences

    Professor James Michael Fleckenstein

  20. Phosphodiesterase 5 (PDE5) restricts intracellular cGMP accumulation during enterotoxigenic Escherichia coli infection

    Article • Gut Microbes, May 2020, Taylor & Francis

    Professor James Michael Fleckenstein

  21. Mechanical Stimuli Affect Escherichia coli Heat-Stable Enterotoxin-Cyclic GMP Signaling in a Human Enteroid Intestine-Chip Model

    Article • Infection and Immunity, December 2019, ASM Journals

    Professor James Michael Fleckenstein

  22. Comparative genomic analysis and molecular examination of the diversity of enterotoxigenic Escherichia coli isolates from Chile

    Article • PLoS Neglected Tropical Diseases, November 2019, PLOS

    Professor James Michael Fleckenstein, Dr David A Rasko

  23. Mechanical Stimuli Affect E. Coli Heat Stable Enterotoxin (ST)-Cyclic GMP Signaling in a Human Enteroid Intestine-Chip Diarrhea Model

    Article • September 2019, Cold Spring Harbor Laboratory Press

    Professor James Michael Fleckenstein

  24. Interrogation of a live-attenuated enterotoxigenic Escherichia coli vaccine highlights features unique to wild-type infection

    Article • npj Vaccines, August 2019, Springer Science + Business Media

    Professor James Michael Fleckenstein

  25. How genomics can be used to understand host susceptibility to enteric infection, aiding in the development of vaccines and immunotherapeutic interventions

    Article • Vaccine, August 2019, Elsevier

    Professor James Michael Fleckenstein

  26. Enterotoxigenic Escherichia coli–blood group A interactions intensify diarrheal severity

    Article • Journal of Clinical Investigation, July 2019, American Society for Clinical Investigation

    Professor James Michael Fleckenstein

  27. Enterotoxigenic Escherichia coli Infections

    Article • Current Infectious Disease Reports, March 2019, Springer Science + Business Media

    Professor James Michael Fleckenstein

  28. Molecular Determinants of Enterotoxigenic Escherichia coli Heat-Stable Toxin Secretion and Delivery

    Article • Infection and Immunity, August 2018, ASM Journals

    Professor James Michael Fleckenstein

  29. Enterotoxigenic Escherichia coli–blood group A interactions intensify diarrheal severity

    Article • Journal of Clinical Investigation, June 2018, American Society for Clinical Investigation

    Professor James Michael Fleckenstein

  30. Human Experimental Challenge With Enterotoxigenic Escherichia coli Elicits Immune Responses to Canonical and Novel Antigens Relevant to Vaccine Development

    Article • The Journal of Infectious Diseases, May 2018, Oxford University Press (OUP)

    Professor James Michael Fleckenstein

  31. Molecular determinants of enterotoxigenic Escherichia coli heat-stable toxin secretion and delivery

    Article • April 2018, Cold Spring Harbor Laboratory Press

    Professor James Michael Fleckenstein

  32. A Role for Salivary Peptides in the Innate Defense Against Enterotoxigenic Escherichia coli

    Article • The Journal of Infectious Diseases, March 2018, Oxford University Press (OUP)

    Professor James Michael Fleckenstein

  33. Characterization of enterotoxigenic Escherichia coli strains isolated from the massive multi-pathogen gastroenteritis outbreak in the Antofagasta region following the Chilean earthquake, 2010

    Article • Infection Genetics and Evolution, August 2017, Elsevier

    Professor James Michael Fleckenstein

  34. Comparative genomics and transcriptomics of Escherichia coli isolates carrying virulence factors of both enteropathogenic and enterotoxigenic E. coli

    Article • Scientific Reports, June 2017, Springer Science + Business Media

    Professor James Michael Fleckenstein

  35. Insights into enterotoxigenic Escherichia coli diversity in Bangladesh utilizing genomic epidemiology

    Article • Scientific Reports, June 2017, Springer Science + Business Media

    Professor James Michael Fleckenstein

  36. Providing Structure to Enterotoxigenic Escherichia coli Vaccine Development

    Article • The Journal of Infectious Diseases, May 2017, Oxford University Press (OUP)

    Professor James Michael Fleckenstein

  37. Highly conserved type 1 pili promote enterotoxigenic E. coli pathogen-host interactions

    Article • PLoS Neglected Tropical Diseases, May 2017, PLOS

    Professor James Michael Fleckenstein

  38. Dynamic Interactions of a Conserved Enterotoxigenic Escherichia coli Adhesin with Intestinal Mucins Govern Epithelium Engagement and Toxin Delivery

    Article • Infection and Immunity, October 2016, ASM Journals

    Professor James Michael Fleckenstein

  39. Blood Group O–Dependent Cellular Responses to Cholera Toxin: Parallel Clinical and Epidemiological Links to Severe Cholera

    Article • American Journal of Tropical Medicine and Hygiene, August 2016, American Society of Tropical Medicine and Hygiene

    Professor James Michael Fleckenstein

  40. Immunogenicity and Protective Efficacy against Enterotoxigenic Escherichia coli Colonization following Intradermal, Sublingual, or Oral Vaccination with EtpA Adhesin

    Article • Clinical and Vaccine Immunology, May 2016, ASM Journals

    Professor James Michael Fleckenstein

  41. Overcoming Enterotoxigenic Escherichia coli Pathogen Diversity: Translational Molecular Approaches to Inform Vaccine Design

    Article • January 2016, Springer Science + Business Media

    Professor James Michael Fleckenstein

  42. Examination of the Enterotoxigenic Escherichia coli Population Structure during Human Infection

    Article • mBio, July 2015, ASM Journals

    Dr David A Rasko, Professor James Michael Fleckenstein

  43. Conservation and Immunogenicity of Novel Antigens in Diverse Isolates of Enterotoxigenic Escherichia coli

    Article • PLoS Neglected Tropical Diseases, January 2015, PLOS

    Dr David A Rasko, Professor James Michael Fleckenstein

  44. Contribution of the Highly Conserved EaeH Surface Protein to Enterotoxigenic Escherichia coli Pathogenesis

    Article • Infection and Immunity, June 2014, ASM Journals

    Professor James Michael Fleckenstein

  45. Designing Vaccines to Neutralize Effective Toxin Delivery by Enterotoxigenic Escherichia coli

    Article • Toxins, June 2014, MDPI AG

    Professor James Michael Fleckenstein

  46. Novel antigens for enterotoxigenicEscherichia colivaccines

    Article • Expert Review of Vaccines, April 2014, Taylor & Francis

    Professor James Michael Fleckenstein

  47. Enterotoxigenic Escherichia coli Secretes a Highly Conserved Mucin-Degrading Metalloprotease To Effectively Engage Intestinal Epithelial Cells

    Article • Infection and Immunity, November 2013, ASM Journals

    Professor James Michael Fleckenstein

  48. EatA, an Immunogenic Protective Antigen of Enterotoxigenic Escherichia coli, Degrades Intestinal Mucin

    Article • Infection and Immunity, November 2013, ASM Journals

    Professor James Michael Fleckenstein

  49. EnterotoxigenicEscherichia coli

    Article • Gut Microbes, September 2013, Taylor & Francis

    Dr David A Rasko, Professor James Michael Fleckenstein

  50. Escherichia coli Sequence Type 131 (ST131) Subclone H30 as an Emergent Multidrug-Resistant Pathogen Among US Veterans

    Article • Clinical Infectious Diseases, August 2013, Oxford University Press (OUP)

    Professor James Michael Fleckenstein

  51. Enterotoxigenic Escherichia coli

    Article • January 2013, Elsevier

    Professor James Michael Fleckenstein

  52. Transcriptional Modulation of Enterotoxigenic Escherichia coli Virulence Genes in Response to Epithelial Cell Interactions

    Article • Infection and Immunity, October 2012, ASM Journals

    Professor James Michael Fleckenstein

  53. Cooperative Role of Antibodies against Heat-Labile Toxin and the EtpA Adhesin in Preventing Toxin Delivery and Intestinal Colonization by Enterotoxigenic Escherichia coli

    Article • Clinical and Vaccine Immunology, August 2012, ASM Journals

    Professor James Michael Fleckenstein

  54. Directed Evaluation of Enterotoxigenic Escherichia coli Autotransporter Proteins as Putative Vaccine Candidates

    Article • PLoS Neglected Tropical Diseases, December 2011, PLOS

    Professor James Michael Fleckenstein

  55. Outer Membrane Vesicles Induce Immune Responses to Virulence Proteins and Protect against Colonization by Enterotoxigenic Escherichia coli

    Article • Clinical and Vaccine Immunology, September 2011, ASM Journals

    Professor James Michael Fleckenstein

  56. Adhesin Degradation Accelerates Delivery of Heat-labile Toxin by EnterotoxigenicEscherichia coli

    Article • Journal of Biological Chemistry, July 2011, American Society for Biochemistry & Molecular Biology (ASBMB)

    Professor James Michael Fleckenstein

  57. Infectious Agents of Food- and Water-Borne Illnesses

    Article • The American Journal of the Medical Sciences, September 2010, Elsevier

    Professor James Michael Fleckenstein

  58. Enterotoxigenic Escherichia coli Elicits Immune Responses to Multiple Surface Proteins

    Article • Infection and Immunity, May 2010, ASM Journals

    Professor James Michael Fleckenstein

  59. Lingual Leishmaniasis Complicating Visceral Disease

    Article • Journal of Travel Medicine, May 2010, Oxford University Press (OUP)

    Professor James Michael Fleckenstein

  60. Molecular mechanisms of enterotoxigenic Escherichia coli infection

    Article • Microbes and Infection, February 2010, Elsevier

    Dr David A Rasko, Professor James Michael Fleckenstein

  61. Vaccination with EtpA glycoprotein or flagellin protects against colonization with enterotoxigenic Escherichia coli in a murine model

    Article • Vaccine, July 2009, Elsevier

    Professor James Michael Fleckenstein

  62. Purification of recombinant high molecular weight two-partner secretion proteins from Escherichia coli

    Article • Nature Protocols, July 2009, Springer Science + Business Media

    Professor James Michael Fleckenstein

  63. Enterotoxigenic Escherichia coli EtpA mediates adhesion between flagella and host cells

    Article • Nature, December 2008, Springer Science + Business Media

    Professor James Michael Fleckenstein

  64. Heat-Labile Enterotoxin Promotes Escherichia coli Adherence to Intestinal Epithelial Cells

    Article • Journal of Bacteriology, October 2008, ASM Journals

    Professor James Michael Fleckenstein

  65. The EtpA Exoprotein of Enterotoxigenic Escherichia coli Promotes Intestinal Colonization and Is a Protective Antigen in an Experimental Model of Murine Infection

    Article • Infection and Immunity, February 2008, ASM Journals

    Professor James Michael Fleckenstein

  66. Directed delivery of heat-labile enterotoxin by enterotoxigenic Escherichia coli

    Article • Cellular Microbiology, September 2006, Wiley

    Professor James Michael Fleckenstein

  67. Identification of a Two-Partner Secretion Locus of Enterotoxigenic Escherichia coli

    Article • Infection and Immunity, April 2006, ASM Journals

    Professor James Michael Fleckenstein

  68. Importance of Heat-Labile Enterotoxin in Colonization of the Adult Mouse Small Intestine by Human Enterotoxigenic Escherichia coli Strains

    Article • Infection and Immunity, February 2006, ASM Journals

    Professor James Michael Fleckenstein

  69. Identification and Molecular Characterization of EatA, an Autotransporter Protein of Enterotoxigenic Escherichia coli

    Article • Infection and Immunity, March 2004, ASM Journals

    Professor James Michael Fleckenstein

  70. Interaction of an Outer Membrane Protein of Enterotoxigenic Escherichia coli with Cell Surface Heparan Sulfate Proteoglycans

    Article • Infection and Immunity, March 2002, ASM Journals

    Professor James Michael Fleckenstein

  71. Breaching the mucosal barrier by stealth: an emerging pathogenic mechanism for enteroadherent bacterial pathogens

    Article • Journal of Clinical Investigation, January 2001, American Society for Clinical Investigation

    Professor James Michael Fleckenstein

  72. Identification of a Gene within a Pathogenicity Island of Enterotoxigenic Escherichia coli H10407 Required for Maximal Secretion of the Heat-Labile Enterotoxin

    Article • Infection and Immunity, May 2000, ASM Journals

    Professor James Michael Fleckenstein

  73. Incidental Discovery of Emphysematous Cystitis

    Article • Southern Medical Journal, August 1998, Southern Medical Association

    Professor James Michael Fleckenstein

  74. Cryptococcal Skull Infection

    Article • Neurosurgery, June 1993, Oxford University Press (OUP)

    Professor James Michael Fleckenstein