All Stories

  1. Crystal structure of a recombinant Agaricus bisporus mushroom mannose-binding protein with a longer C-terminal region

    Article • Acta Crystallographica Section F Structural Biology Communications, May 2025, International Union of Crystallography

    Hiromi Yoshida

  2. L-rhamnose isomerase: a crucial enzyme for rhamnose catabolism and conversion of rare sugars

    Article • Applied Microbiology and Biotechnology, October 2024, Springer Science + Business Media

    Hiromi Yoshida

  3. X-ray structure and characterization of a probiotic Lactobacillus rhamnosus Probio-M9 L-rhamnose isomerase

    Article • Applied Microbiology and Biotechnology, March 2024, Springer Science + Business Media

    Hiromi Yoshida

  4. Direct relationship between dimeric form and activity in the acidic copper–zinc superoxide dismutase from lemon

    Article • Acta Crystallographica Section F Structural Biology Communications, December 2023, International Union of Crystallography

    Hiromi Yoshida

  5. Introducing Intermolecular Interaction to Strengthen the Stability of MnSOD Dimer

    Article • Applied Biochemistry and Biotechnology, January 2023, Springer Science + Business Media

    Hiromi Yoshida

  6. Microgravity environment grown crystal structure information based engineering of direct electron transfer type glucose dehydrogenase

    Article • Communications Biology, December 2022, Springer Science + Business Media

    Hiromi Yoshida

  7. Structure of lactate oxidase from Enterococcus hirae revealed new aspects of active site loop function: Product‐inhibition mechanism and oxygen gatekeeper

    Article • Protein Science, September 2022, Wiley

    Hiromi Yoshida

  8. Crystal structure of a novel homodimeric l ‐ribulose 3‐epimerase from Methylomonus sp.

    Article • FEBS Open Bio, May 2021, Wiley

    Hiromi Yoshida

  9. Unprecedented Role of the N73-F124 Pair in the Staphylococcus equorum MnSOD Activity

    Article • Current Enzyme Inhibition, March 2021, Bentham Science Publishers

    Anita Artarini, Andrian Hartanto, Hiromi Yoshida

  10. X-ray structure of the direct electron transfer-type FAD glucose dehydrogenase catalytic subunit complexed with a hitchhiker protein

    Article • Acta Crystallographica Section D Structural Biology, August 2019, International Union of Crystallography

    Hiromi Yoshida

  11. Designer fungus FAD glucose dehydrogenase capable of direct electron transfer

    Article • Biosensors and Bioelectronics, January 2019, Elsevier

    Hiromi Yoshida

  12. Engineered fungus derived FAD-dependent glucose dehydrogenase with acquired ability to utilize hexaammineruthenium(III) as an electron acceptor

    Article • Bioelectrochemistry, October 2018, Elsevier

    Hiromi Yoshida

  13. X-ray structure of Arthrobacter globiformis M30 ketose 3-epimerase for the production of D-allulose from D-fructose

    Article • Acta Crystallographica Section F Structural Biology Communications, September 2018, International Union of Crystallography

    Hiromi Yoshida

  14. The first crystal structure of manganese superoxide dismutase from the genusStaphylococcus

    Article • Acta Crystallographica Section F Structural Biology Communications, February 2018, International Union of Crystallography

    Hiromi Yoshida

  15. X-ray structure of Clostridium perfringens sortase B cysteine transpeptidase

    Article • Biochemical and Biophysical Research Communications, November 2017, Elsevier

    Hiromi Yoshida

  16. X-ray structure of a protease-resistant mutant form of human galectin-9 having two carbohydrate recognition domains with a metal-binding site

    Article • Biochemical and Biophysical Research Communications, September 2017, Elsevier

    Hiromi Yoshida

  17. X-ray structures of fructosyl peptide oxidases revealing residues responsible for gating oxygen access in the oxidative half reaction

    Article • Scientific Reports, June 2017, Springer Science + Business Media

    Hiromi Yoshida

  18. Role of the Tyr270 residue in 2-methyl-3-hydroxypyridine-5-carboxylic acid oxygenase from Mesorhizobium loti

    Article • Journal of Bioscience and Bioengineering, February 2017, Elsevier

    Hiromi Yoshida

  19. Structural and biochemical characterization of theClostridium perfringensautolysin catalytic domain

    Article • FEBS Letters, December 2016, Wiley

    Hiromi Yoshida

  20. X-ray structures of the Pseudomonas cichorii D-tagatose 3-epimerase mutant form C66S recognizing deoxy sugars as substrates

    Article • Applied Microbiology and Biotechnology, July 2016, Springer Science + Business Media

    Hiromi Yoshida

  21. Structural analysis of fungus-derived FAD glucose dehydrogenase

    Article • Scientific Reports, August 2015, Springer Science + Business Media

    Hiromi Yoshida

  22. Crystal structure of a Xenopus laevis skin proto-type galectin, close to but distinct from galectin-1

    Article • Glycobiology, March 2015, Oxford University Press (OUP)

    Hiromi Yoshida

  23. Essentiality of tetramer formation of Cellulomonas parahominis L-ribose isomerase involved in novel L-ribose metabolic pathway

    Article • Applied Microbiology and Biotechnology, February 2015, Springer Science + Business Media

    Hiromi Yoshida

  24. X-ray structure of a novell-ribose isomerase acting on a non-natural sugarl-ribose as its ideal substrate

    Article • European Journal of Biochemistry, June 2014, Wiley

    Hiromi Yoshida

  25. X-ray structure of a novel endolysin encoded by episomal phage phiSM101 ofClostridium perfringens

    Article • Molecular Microbiology, March 2014, Wiley

    Hiromi Yoshida

  26. Purification, crystallization and preliminary X-ray analysis of an HA17–HA70 (HA2–HA3) complex fromClostridium botulinumtype C progenitor toxin

    Article • Acta Crystallographica Section F Structural Biology Communications, December 2013, International Union of Crystallography

    Hiromi Yoshida

  27. Structure ofl-rhamnose isomerase in complex withl-rhamnopyranose demonstrates the sugar-ring opening mechanism and the role of a substrate sub-binding site

    Article • FEBS Open Bio, December 2012, Wiley

    Hiromi Yoshida

  28. X-ray structure of a protease-resistant mutant form of human galectin-8 with two carbohydrate recognition domains

    Article • European Journal of Biochemistry, September 2012, Wiley

    Hiromi Yoshida

  29. Carbohydrate recognition mechanism of HA70 fromClostridium botulinumdeduced from X-ray structures in complexes with sialylated oligosaccharides

    Article • FEBS Letters, June 2012, Wiley

    Hiromi Yoshida

  30. Overexpression, crystallization and preliminary X-ray diffraction analysis ofL-ribose isomerase fromAcinetobactersp. strain DL-28

    Article • Acta Crystallographica Section F Structural Biology and Crystallization Communications, September 2011, International Union of Crystallography

    Hiromi Yoshida

  31. Crystal structures of rare disaccharides, α-d-glucopyranosyl β-d-psicofuranoside, and α-d-galactopyranosyl β-d-psicofuranoside

    Article • Carbohydrate Research, July 2011, Elsevier

    Hiromi Yoshida

  32. Monooxygenation by a thermophilic cytochrome P450 via direct electron donation from NADH

    Article • Metallomics, January 2011, Oxford University Press (OUP)

    Professor Masafumi Yohda, Hiromi Yoshida, PhD Nobuhumi Nakamura, Hiroyuki Ohno

  33. Elucidation of the role of Ser329 and the C-terminal region in the catalytic activity of Pseudomonas stutzeril-rhamnose isomerase

    Article • Protein Engineering Design and Selection, October 2010, Oxford University Press (OUP)

    Hiromi Yoshida

  34. X-ray Structures of Human Galectin-9 C-terminal Domain in Complexes with a Biantennary Oligosaccharide and Sialyllactose

    Article • Journal of Biological Chemistry, September 2010, American Society for Biochemistry & Molecular Biology (ASBMB)

    Hiromi Yoshida

  35. X-ray structures of Bacillus pallidus d-arabinose isomerase and its complex with l-fucitol

    Article • Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics, June 2010, Elsevier

    Hiromi Yoshida

  36. Structures of an Isopenicillin N Converting Ntn-Hydrolase Reveal Different Catalytic Roles for the Active Site Residues of Precursor and Mature Enzyme

    Article • Structure, March 2010, Elsevier

    Hiromi Yoshida

  37. Catalytic reaction mechanism of Pseudomonas stutzeri l-rhamnose isomerase deduced from X-ray structures

    Article • European Journal of Biochemistry, January 2010, Wiley

    Hiromi Yoshida

  38. Crystallization and preliminary X-ray analysis of AAMS amidohydrolase, the final enzyme in degradation pathway I of pyridoxine

    Article • Acta Crystallographica Section F Structural Biology and Crystallization Communications, July 2009, International Union of Crystallography

    Hiromi Yoshida

  39. Crystal structures of open and closed forms of cyclo/maltodextrin-binding protein

    Article • European Journal of Biochemistry, June 2009, Wiley

    Hiromi Yoshida

  40. Crystallization and preliminary X-ray diffraction analysis of a protease-resistant mutant form of human galectin-8

    Article • Acta Crystallographica Section F Structural Biology and Crystallization Communications, April 2009, International Union of Crystallography

    Hiromi Yoshida

  41. β-D-Altrose

    Article • Acta Crystallographica Section E Structure Reports Online, January 2009, International Union of Crystallography

    Hiromi Yoshida

  42. Corrigendum to “Structural Insights into the Substrate Specificity and Function of Escherichia coli K12 YgjK, a Glucosidase Belonging to the Glycoside Hydrolase Family 63” [J. Mol. Biol. 381 (2008) 116–128]

    Article • Journal of Molecular Biology, November 2008, Elsevier

    Hiromi Yoshida

  43. Overexpression, purification, crystallization and preliminary X-ray crystal analysis ofBacillus pallidusD-arabinose isomerase

    Article • Acta Crystallographica Section F Structural Biology and Crystallization Communications, September 2008, International Union of Crystallography

    Hiromi Yoshida

  44. Functional and structural bases of a cysteine-less mutant as a long-lasting substitute for galectin-1

    Article • Glycobiology, September 2008, Oxford University Press (OUP)

    Hiromi Yoshida

  45. Structural Insights into the Substrate Specificity and Function of Escherichia coli K12 YgjK, a Glucosidase Belonging to the Glycoside Hydrolase Family 63

    Article • Journal of Molecular Biology, August 2008, Elsevier

    Hiromi Yoshida

  46. Structure of a putative molybdenum-cofactor biosynthesis protein C (MoaC) fromSulfolobus tokodaii(ST0472)

    Article • Acta Crystallographica Section F Structural Biology and Crystallization Communications, June 2008, International Union of Crystallography

    Hiromi Yoshida

  47. Crystal Structure of Aspergillus niger Isopullulanase, a Member of Glycoside Hydrolase Family 49

    Article • Journal of Molecular Biology, February 2008, Elsevier

    Hiromi Yoshida

  48. Crystal Structures of d-Tagatose 3-Epimerase from Pseudomonas cichorii and Its Complexes with d-Tagatose and d-Fructose

    Article • Journal of Molecular Biology, November 2007, Elsevier

    Hiromi Yoshida

  49. Structural basis for cyclodextrin recognition by Thermoactinomyces vulgaris cyclo/maltodextrin-binding protein

    Article • European Journal of Biochemistry, March 2007, Wiley

    Hiromi Yoshida

  50. The Structures of l-Rhamnose Isomerase from Pseudomonas stutzeri in Complexes with l-Rhamnose and d-Allose Provide Insights into Broad Substrate Specificity

    Article • Journal of Molecular Biology, February 2007, Elsevier

    Hiromi Yoshida

  51. Purification, crystallization and preliminary X-ray diffraction studies ofD-tagatose 3-epimerase fromPseudomonas cichorii

    Article • Acta Crystallographica Section F Structural Biology and Crystallization Communications, January 2007, International Union of Crystallography

    Hiromi Yoshida

  52. Structure of a complex of Thermoactinomyces vulgaris R-47 α-amylase 2 with maltohexaose demonstrates the important role of aromatic residues at the reducing end of the substrate binding cleft

    Article • Carbohydrate Research, June 2006, Elsevier

    Hiromi Yoshida

  53. Crystallization and preliminary X-ray diffraction studies ofL-rhamnose isomerase fromPseudomonas stutzeri

    Article • Acta Crystallographica Section F Structural Biology and Crystallization Communications, May 2006, International Union of Crystallography

    Hiromi Yoshida

  54. Complexes of Thermoactinomyces vulgaris R-47 α-amylase 1 and pullulan model oligossacharides provide new insight into the mechanism for recognizing substrates with α-(1,6) glycosidic linkages

    Article • European Journal of Biochemistry, November 2005, Wiley

    Hiromi Yoshida

  55. An approach to prevent aggregation during the purification and crystallization of wild type acyl coenzyme A: Isopenicillin N acyltransferase from Penicillium chrysogenum

    Article • Protein Expression and Purification, May 2005, Elsevier

    Hiromi Yoshida

  56. Three-dimensional structures of enzymes useful for ?-lactam antibiotic production

    Article • Current Opinion in Biotechnology, August 2004, Elsevier

    Hiromi Yoshida

  57. Significantly enhanced stability of glucose dehydrogenase by directed evolution

    Article • Applied Microbiology and Biotechnology, March 2003, Springer Science + Business Media

    Hiromi Yoshida

  58. Article • Biotechnology Letters, January 2003, Springer Science + Business Media

    Hiromi Yoshida

  59. Article • Biotechnology Letters, January 2002, Springer Science + Business Media

    Hiromi Yoshida

  60. Increasing the thermal stability of the water-soluble pyrroloquinoline quinone glucose dehydrogenase by single amino acid replacement

    Article • Enzyme and Microbial Technology, April 2000, Elsevier

    Hiromi Yoshida

  61. Construction and Characterization of Mutant Water-Soluble PQQ Glucose Dehydrogenases with Altered Km Values—Site-Directed Mutagenesis Studies on the Putative Active Site

    Article • Biochemical and Biophysical Research Communications, November 1999, Elsevier

    Hiromi Yoshida

  62. Engineering a chimeric pyrroloquinoline quinone glucose dehydrogenase: improvement of EDTA tolerance, thermal stability and substrate specificity

    Article • Protein Engineering Design and Selection, January 1999, Oxford University Press (OUP)

    Hiromi Yoshida

  63. Inducer exclusion in Escherichia coli by non-PTS substrates: the role of the PEP to pyruvate ratio in determining the phosphorylation state of enzyme IIAGlc

    Article • Molecular Microbiology, November 1998, Wiley

    Hiromi Yoshida

  64. Increased production of recombinant pyrroloquinoline quinone (PQQ) glucose dehydrogenase by metabolically engineered Escherichia coli strain capable of PQQ biosynthesis

    Article • Journal of Biotechnology, August 1996, Elsevier

    Hiromi Yoshida

  65. Elucidation of the Region Responsible for EDTA Tolerance in PQQ Glucose Dehydrogenases by Constructing Escherichia coli and Acinetobacter calcoaceticus Chimeric Enzymes

    Article • Biochemical and Biophysical Research Communications, June 1995, Elsevier

    Hiromi Yoshida