All Stories

  1. Crystal structure of UDP-glucose pyrophosphorylase fromYersinia pestis, a potential therapeutic target against plague

    Article • Acta Crystallographica Section F Structural Biology Communications, August 2019, International Union of Crystallography

    Dr David S Waugh

  2. High-resolution crystal structures of the D1 and D2 domains of protein tyrosine phosphatase epsilon for structure-based drug design

    Article • Acta Crystallographica Section D Structural Biology, October 2018, International Union of Crystallography

    Dr David S Waugh

  3. Discovery of small molecule inhibitors of MyD88-dependent signaling pathways using a computational screen

    Article • Scientific Reports, September 2015, Nature

    Dr David S Waugh

  4. Structure of human dual-specificity phosphatase 7, a potential cancer drug target

    Article • Acta Crystallographica Section F Structural Biology Communications, May 2015, International Union of Crystallography

    Dr David S Waugh

  5. Structures of theMiddle East respiratory syndrome coronavirus3C-like protease reveal insights into substrate specificity

    Article • Acta Crystallographica Section D Biological Crystallography, April 2015, International Union of Crystallography

    Dr David S Waugh

  6. Structural analysis of human dual-specificity phosphatase 22 complexed with a phosphotyrosine-like substrate

    Article • Acta Crystallographica Section F Structural Biology Communications, January 2015, International Union of Crystallography

    Dr David S Waugh

  7. Rescuing Aggregation-Prone Proteins in Escherichia coli with a Dual His6-MBP Tag

    Article • January 2014, Springer Science + Business Media

    Dr David S Waugh

  8. Differential temperature dependence of tobacco etch virus and rhinovirus 3C proteases

    Article • Analytical Biochemistry, May 2013, Elsevier

    Dr David S Waugh

  9. Isolation of Metarhizium anisopliae carboxypeptidase A with native disulfide bonds from the cytosol of Escherichia coli BL21(DE3)

    Article • Protein Expression and Purification, March 2012, Elsevier

    Dr David S Waugh

  10. An overview of enzymatic reagents for the removal of affinity tags

    Article • Protein Expression and Purification, December 2011, Elsevier

    Dr David S Waugh

  11. Reprint of: Making the most of affinity tags

    Article • Protein Expression and Purification, September 2011, Elsevier

    Dr David S Waugh

  12. Enhancing the Solubility of Recombinant Proteins in Escherichia coli by Using Hexahistidine-Tagged Maltose-Binding Protein as a Fusion Partner

    Article • November 2010, Springer Science + Business Media

    Dr David S Waugh

  13. Structural determinants of tobacco vein mottling virus protease substrate specificity

    Article • Protein Science, October 2010, Wiley

    Dr David S Waugh

  14. Overproduction, Purification and Structure Determination of Human Dual Specificity Phosphatase 14

    Article • Biophysical Journal, January 2010, Elsevier

    Dr David S Waugh

  15. Crystal structure of the Yersinia pestis GTPase activator YopE

    Article • Protein Science, April 2009, Wiley

    Dr Artem Evdokimov, Dr David S Waugh

  16. Molecular cloning, overproduction, purification and biochemical characterization of the p39 nsp2 protease domains encoded by three alphaviruses

    Article • Protein Expression and Purification, March 2009, Elsevier

    Dr David S Waugh

  17. Structural Basis for Binding of RNA and Cofactor by a KsgA Methyltransferase

    Article • Structure, March 2009, Elsevier

    Dr David S Waugh

  18. Atomic resolution structure of the cytoplasmic domain ofYersinia pestisYscU, a regulatory switch involved in type III secretion

    Article • Protein Science, February 2009, Wiley

    Dr David S Waugh

  19. Expression and Purification of Soluble His6-Tagged TEV Protease

    Article • January 2009, Springer Science + Business Media

    Dr David S Waugh

  20. Hexahistidine-Tagged Maltose-Binding Protein as a Fusion Partner for the Production of Soluble Recombinant Proteins in Escherichia coli

    Article • January 2009, Springer Science + Business Media

    Dr David S Waugh

  21. Crystal structure of the protease-resistant core domain of Yersinia pestis virulence factor YopR

    Article • Protein Science, January 2009, Wiley

    Dr David S Waugh

  22. Novel fold of VirA, a type III secretion system effector protein from Shigella flexneri

    Article • Protein Science, December 2008, Wiley

    Dr David S Waugh

  23. A stepwise model for double-stranded RNA processing by ribonuclease III

    Article • Molecular Microbiology, November 2007, Wiley

    Dr David S Waugh

  24. New protein fold revealed by a 1.65 Å resolution crystal structure ofFrancisella tularensispathogenicity island protein IglC

    Article • Protein Science, November 2007, Wiley

    Dr David S Waugh

  25. A generic protocol for the expression and purification of recombinant proteins in Escherichia coli using a combinatorial His6-maltose binding protein fusion tag

    Article • Nature Protocols, March 2007, Nature

    Dr David S Waugh

  26. Structure of the POZ domain of human LRF, a master regulator of oncogenesis

    Article • Biochemical and Biophysical Research Communications, December 2006, Elsevier

    Dr David S Waugh

  27. Overproduction, purification, and biochemical characterization of the dual specificity H1 protein phosphatase encoded by variola major virus

    Article • Protein Expression and Purification, November 2006, Elsevier

    Dr David S Waugh

  28. Structural Insight into the Mechanism of Double-Stranded RNA Processing by Ribonuclease III

    Article • Cell, January 2006, Elsevier

    Dr David S Waugh

  29. Solubility-enhancing proteins MBP and NusA play a passive role in the folding of their fusion partners

    Article • Protein Expression and Purification, January 2006, Elsevier

    Dr David S Waugh

  30. Crystal structure of theYersiniatype III secretion protein YscE

    Article • Protein Science, October 2005, Wiley

    Dr David S Waugh

  31. Intermediate States of Ribonuclease III in Complex with Double-Stranded RNA

    Article • Structure, October 2005, Elsevier

    Dr David S Waugh

  32. Selection and characterization of Yersinia pestis YopN mutants that constitutively block Yop secretion

    Article • Molecular Microbiology, June 2005, Wiley

    Dr David S Waugh

  33. Making the most of affinity tags

    Article • Trends in Biotechnology, June 2005, Elsevier

    Dr David S Waugh

  34. Comparison of the substrate specificity of two potyvirus proteases

    Article • FEBS Journal, December 2004, Wiley

    Dr David S Waugh

  35. Involvement of Domain II in Toxicity of Anthrax Lethal Factor

    Article • Journal of Biological Chemistry, October 2004, American Society for Biochemistry & Molecular Biology (ASBMB)

    Dr David S Waugh

  36. Noncatalytic Assembly of Ribonuclease III with Double-Stranded RNA

    Article • Structure, March 2004, Elsevier

    Dr David S Waugh

  37. Maltodextrin-binding proteins from diverse bacteria and archaea are potent solubility enhancers

    Article • FEBS Letters, January 2003, Wiley

    Dr David S Waugh

  38. The P1′ specificity of tobacco etch virus protease

    Article • Biochemical and Biophysical Research Communications, June 2002, Elsevier

    Dr David S Waugh

  39. Structural basis for oligosaccharide recognition by Pyrococcus furiosus maltodextrin-binding protein

    Article • Journal of Molecular Biology, January 2001, Elsevier

    Dr Artem Evdokimov, Dr David S Waugh

  40. Controlled Intracellular Processing of Fusion Proteins by TEV Protease

    Article • Protein Expression and Purification, July 2000, Elsevier

    Dr David S Waugh

  41. Crystal structure of plant aspartic proteinase prophytepsin: inactivation and vacuolar targeting

    Article • The EMBO Journal, July 1999, Wiley

    Dr David S Waugh

  42. Escherichia colimaltose-binding protein is uncommonly effective at promoting the solubility of polypeptides to which it is fused

    Article • Protein Science, January 1999, Wiley

    Dr David S Waugh

  43. Site-Specific, Enzymatic Biotinylation of Recombinant Proteins inSpodoptera frugiperdaCells Using Biotin Acceptor Peptides

    Article • Analytical Biochemistry, September 1998, Elsevier

    Dr David S Waugh

  44. Balancing the Production of Two Recombinant Proteins inEscherichia coliby Manipulating Plasmid Copy Number: High-Level Expression of Heterodimeric Ras Farnesyltransferase

    Article • Protein Expression and Purification, December 1997, Elsevier

    Dr David S Waugh

  45. A versatile plasmid expression vector for the production of biotinylated proteins by site-specific, enzymatic modification in Escherichia coli

    Article • Gene, February 1996, Elsevier

    Dr David S Waugh

  46. Expression of Soluble Human Interleukin-2 Receptor α-Chain inEscherichia coli

    Article • Protein Expression and Purification, December 1995, Elsevier

    Dr David S Waugh

  47. [26] Design of simplified ribonuclease P RNA by phylogenetic comparison

    Article • January 1991, Elsevier

    Dr David S Waugh

  48. Bacillus subtilis RNase P

    Article • January 1987, Elsevier

    Dr David S Waugh

  49. Two electrophoresis experiments for freshmen in the health professions

    Article • Journal of Chemical Education, June 1986, American Chemical Society (ACS)

    Dr David S Waugh

  50. Catalysis by RNA

    Article • BioEssays, February 1986, Wiley

    Dr David S Waugh

  51. Faculty of 1000 evaluation for In cellulo structure determination of a novel cypovirus polyhedrin.

    Article • Faculty of 1000, Ltd.

    Dr David S Waugh

  52. Faculty of 1000 evaluation for Slowing bacterial translation speed enhances eukaryotic protein folding efficiency.

    Article • Faculty of 1000, Ltd.

    Dr David S Waugh

  53. Faculty of 1000 evaluation for An approach to crystallizing proteins by metal-mediated synthetic symmetrization.

    Article • Faculty of 1000, Ltd.

    Dr David S Waugh

  54. Faculty of 1000 evaluation for Atomic model of the type III secretion system needle.

    Article • Faculty of 1000, Ltd.

    Dr David S Waugh

  55. Faculty of 1000 evaluation for Fragment-based discovery of JAK-2 inhibitors.

    Article • Faculty of 1000, Ltd.

    Dr David S Waugh

  56. Faculty of 1000 evaluation for Crystallization of small proteins assisted by green fluorescent protein.

    Article • Faculty of 1000, Ltd.

    Dr David S Waugh

  57. Faculty of 1000 evaluation for Protein isoelectric point as a predictor for increased crystallization screening efficiency.

    Article • Faculty of 1000, Ltd.

    Dr David S Waugh

  58. Faculty of 1000 evaluation for High-affinity binders selected from designed ankyrin repeat protein libraries.

    Article • Faculty of 1000, Ltd.

    Dr David S Waugh

  59. Faculty of 1000 evaluation for Soluble domains of telomerase reverse transcriptase identified by high-throughput screening.

    Article • Faculty of 1000, Ltd.

    Dr David S Waugh

  60. Faculty of 1000 evaluation for An evaluation of automated model-building procedures for protein crystallography.

    Article • Faculty of 1000, Ltd.

    Dr David S Waugh

  61. Faculty of 1000 evaluation for Targeting bacterial virulence: inhibitors of type III secretion in Yersinia.

    Article • Faculty of 1000, Ltd.

    Dr David S Waugh

  62. Faculty of 1000 evaluation for A new phylum of Archaea represented by a nanosized hyperthermophilic symbiont.

    Article • Faculty of 1000, Ltd.

    Dr David S Waugh

  63. Faculty of 1000 evaluation for Targeting novel folds for structural genomics.

    Article • Faculty of 1000, Ltd.

    Dr David S Waugh

  64. Maltose-Binding Protein as a Solubility Enhancer

    Article • Springer Science + Business Media

    Dr David S Waugh

  65. Faculty of 1000 evaluation for Structural basis of glmS ribozyme activation by glucosamine-6-phosphate.

    Article • Faculty of 1000, Ltd.

    Dr David S Waugh

  66. Faculty of 1000 evaluation for Lysine methylation as a routine rescue strategy for protein crystallization.

    Article • Faculty of 1000, Ltd.

    Dr David S Waugh

  67. Faculty of 1000 evaluation for Protein crystallization by surface entropy reduction: optimization of the SER strategy.

    Article • Faculty of 1000, Ltd.

    Dr David S Waugh

  68. Faculty of 1000 evaluation for Structural basis for double-stranded RNA processing by Dicer.

    Article • Faculty of 1000, Ltd.

    Dr David S Waugh

  69. Faculty of 1000 evaluation for Highly efficient selenomethionine labeling of recombinant proteins produced in mammalian cells.

    Article • Faculty of 1000, Ltd.

    Dr David S Waugh

  70. Faculty of 1000 evaluation for Yersinia YopJ acetylates and inhibits kinase activation by blocking phosphorylation.

    Article • Faculty of 1000, Ltd.

    Dr David S Waugh

  71. Faculty of 1000 evaluation for Detecting allosteric sites of HIV-1 reverse transcriptase by X-ray crystallographic fragment screening.

    Article • Faculty of 1000, Ltd.

    Dr David S Waugh

  72. Faculty of 1000 evaluation for Surface Reengineering of RPA70N Enables Cocrystallization with an Inhibitor of the Replication Protein A Interaction Motif of ATR Interacting Protein.

    Article • Faculty of 1000, Ltd.

    Dr David S Waugh

  73. Faculty of 1000 evaluation for Use of europium ions for SAD phasing of lysozyme at the Cu Kα wavelength.

    Article • Faculty of 1000, Ltd.

    Dr David S Waugh

  74. Faculty of 1000 evaluation for Mass spectrometry guided in situ proteolysis to obtain crystals for X-ray structure determination.

    Article • Faculty of 1000, Ltd.

    Dr David S Waugh

  75. Faculty of 1000 evaluation for IpaB-IpgC interaction defines binding motif for type III secretion translocator.

    Article • Faculty of 1000, Ltd.

    Dr David S Waugh

  76. Faculty of 1000 evaluation for Flexibility of GroES mobile loop is required for efficient chaperonin function.

    Article • Faculty of 1000, Ltd.

    Dr David S Waugh

  77. Faculty of 1000 evaluation for Crystallization of a pentapeptide-repeat protein by reductive cyclic pentylation of free amines with glutaraldehyde.

    Article • Faculty of 1000, Ltd.

    Dr David S Waugh

  78. Faculty of 1000 evaluation for Rapid refinement of crystallographic protein construct definition employing enhanced hydrogen/deuterium exchange MS.

    Article • Faculty of 1000, Ltd.

    Dr David S Waugh

  79. Faculty of 1000 evaluation for On the use of DXMS to produce more crystallizable proteins: structures of the T. maritima proteins TM0160 and TM1171.

    Article • Faculty of 1000, Ltd.

    Dr David S Waugh

  80. Faculty of 1000 evaluation for NMR structure of Mistic, a membrane-integrating protein for membrane protein expression.

    Article • Faculty of 1000, Ltd.

    Dr David S Waugh

  81. Faculty of 1000 evaluation for Crystal structure of a zinc-finger-RNA complex reveals two modes of molecular recognition.

    Article • Faculty of 1000, Ltd.

    Dr David S Waugh

  82. Faculty of 1000 evaluation for Aurintricarboxylic acid blocks in vitro and in vivo activity of YopH, an essential virulent factor of Yersinia pestis, the agent of plague.

    Article • Faculty of 1000, Ltd.

    Dr David S Waugh

  83. Faculty of 1000 evaluation for A method for prediction of the locations of linker regions within large multifunctional proteins, and application to a type I polyketide synthase.

    Article • Faculty of 1000, Ltd.

    Dr David S Waugh

  84. Faculty of 1000 evaluation for Solution structure and phosphopeptide binding to the N-terminal domain of Yersinia YopH: comparison with a crystal structure.

    Article • Faculty of 1000, Ltd.

    Dr David S Waugh

  85. Faculty of 1000 evaluation for Residues participating in the protein folding nucleus do not exhibit preferential evolutionary conservation.

    Article • Faculty of 1000, Ltd.

    Dr David S Waugh

  86. Faculty of 1000 evaluation for The site-specific incorporation of p-iodo-L-phenylalanine into proteins for structure determination.

    Article • Faculty of 1000, Ltd.

    Dr David S Waugh

  87. Faculty of 1000 evaluation for Optimum solubility (OS) screening: an efficient method to optimize buffer conditions for homogeneity and crystallization of proteins.

    Article • Faculty of 1000, Ltd.

    Dr David S Waugh

  88. Faculty of 1000 evaluation for Toward rational protein crystallization: A Web server for the design of crystallizable protein variants.

    Article • Faculty of 1000, Ltd.

    Dr David S Waugh

  89. Faculty of 1000 evaluation for Improving protein solubility: the use of the Escherichia coli dihydrofolate reductase gene as a fusion reporter.

    Article • Faculty of 1000, Ltd.

    Dr David S Waugh

  90. Faculty of 1000 evaluation for An expression vector tailored for large-scale, high-throughput purification of recombinant proteins.

    Article • Faculty of 1000, Ltd.

    Dr David S Waugh

  91. Faculty of 1000 evaluation for Genetic selection for protein solubility enabled by the folding quality control feature of the twin-arginine translocation pathway.

    Article • Faculty of 1000, Ltd.

    Dr David S Waugh

  92. Faculty of 1000 evaluation for Fragment-based substrate activity screening method for the identification of potent inhibitors of the Mycobacterium tuberculosis phosphatase PtpB.

    Article • Faculty of 1000, Ltd.

    Dr David S Waugh

  93. Faculty of 1000 evaluation for Toward chaperone-assisted crystallography: protein engineering enhancement of crystal packing and X-ray phasing capabilities of a camelid single-domain antibody (VHH) scaffold.

    Article • Faculty of 1000, Ltd.

    Dr David S Waugh

  94. Faculty of 1000 evaluation for Folding kinetics and thermodynamics of Pseudomonas syringae effector protein AvrPto provide insight into translocation via the type III secretion system.

    Article • Faculty of 1000, Ltd.

    Dr David S Waugh

  95. Faculty of 1000 evaluation for A Maltose-Binding Protein Fusion Construct Yields a Robust Crystallography Platform for MCL1.

    Article • Faculty of 1000, Ltd.

    Dr David S Waugh